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β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis

An important causative factor in osteoarthritis (OA) is the abnormal mechanical stress-induced bone remodeling of the subchondral bone. β2-adrenergic receptor (Adrb2) plays a major role in mechanical stresses that induce bone remodeling. The medial tibial plateau (MTP) and lateral tibial plateau (LT...

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Autores principales: Yang, Xiaochun, Liang, Xuegang, Guo, Haohui, Ma, Long, Jian, Li, Zhao, Xin, Wang, Jian, Yang, Lvlin, Meng, Zhiqiang, Jin, Qunhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175016/
https://www.ncbi.nlm.nih.gov/pubmed/35794997
http://dx.doi.org/10.1515/med-2022-0498
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author Yang, Xiaochun
Liang, Xuegang
Guo, Haohui
Ma, Long
Jian, Li
Zhao, Xin
Wang, Jian
Yang, Lvlin
Meng, Zhiqiang
Jin, Qunhua
author_facet Yang, Xiaochun
Liang, Xuegang
Guo, Haohui
Ma, Long
Jian, Li
Zhao, Xin
Wang, Jian
Yang, Lvlin
Meng, Zhiqiang
Jin, Qunhua
author_sort Yang, Xiaochun
collection PubMed
description An important causative factor in osteoarthritis (OA) is the abnormal mechanical stress-induced bone remodeling of the subchondral bone. β2-adrenergic receptor (Adrb2) plays a major role in mechanical stresses that induce bone remodeling. The medial tibial plateau (MTP) and lateral tibial plateau (LTP) of patients with varus Knee osteoarthritis (KO) bear different mechanical stresses. The present study aimed to investigate the expression of Adrb2 in medial tibial plateau subchondral bone (MTPSB) and lateral tibial plateau subchondral bone (LTPSB) in patients with varus KO. A total of 30 tibial plateau samples from patients undergoing total knee arthroplasty for varus KO and MTPSB and LTPSB were studied. Statistical analysis was performed using paired sample t-tests. Safranin O-Fast Green staining and Micro-computed tomography showed significant differences in the bone structure between MTPSB and LTPSB. Tartrate-resistant acid phosphatase (TRAP)-positive cell density in MTPSB was higher than that in LTPSB. Immunohistochemistry, reverse transcription-quantitative polymerase chain reaction, and Western blot analysis revealed that compared to LTPSB, the levels of Adrb2, tyrosine hydroxylase (TH), and osteocalcin increased significantly in MTPSB. Double-labeling immunofluorescence showed Adrb2 was present in the majority of TRAP-positive multinuclear cells of the MTPSB. The expression of Adrb2 and TH was significantly higher in MTPSB than in LTPSB, confirming the involvement of these molecules in the development of OA.
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spelling pubmed-91750162022-07-05 β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis Yang, Xiaochun Liang, Xuegang Guo, Haohui Ma, Long Jian, Li Zhao, Xin Wang, Jian Yang, Lvlin Meng, Zhiqiang Jin, Qunhua Open Med (Wars) Research Article An important causative factor in osteoarthritis (OA) is the abnormal mechanical stress-induced bone remodeling of the subchondral bone. β2-adrenergic receptor (Adrb2) plays a major role in mechanical stresses that induce bone remodeling. The medial tibial plateau (MTP) and lateral tibial plateau (LTP) of patients with varus Knee osteoarthritis (KO) bear different mechanical stresses. The present study aimed to investigate the expression of Adrb2 in medial tibial plateau subchondral bone (MTPSB) and lateral tibial plateau subchondral bone (LTPSB) in patients with varus KO. A total of 30 tibial plateau samples from patients undergoing total knee arthroplasty for varus KO and MTPSB and LTPSB were studied. Statistical analysis was performed using paired sample t-tests. Safranin O-Fast Green staining and Micro-computed tomography showed significant differences in the bone structure between MTPSB and LTPSB. Tartrate-resistant acid phosphatase (TRAP)-positive cell density in MTPSB was higher than that in LTPSB. Immunohistochemistry, reverse transcription-quantitative polymerase chain reaction, and Western blot analysis revealed that compared to LTPSB, the levels of Adrb2, tyrosine hydroxylase (TH), and osteocalcin increased significantly in MTPSB. Double-labeling immunofluorescence showed Adrb2 was present in the majority of TRAP-positive multinuclear cells of the MTPSB. The expression of Adrb2 and TH was significantly higher in MTPSB than in LTPSB, confirming the involvement of these molecules in the development of OA. De Gruyter 2022-06-07 /pmc/articles/PMC9175016/ /pubmed/35794997 http://dx.doi.org/10.1515/med-2022-0498 Text en © 2022 Xiaochun Yang et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Yang, Xiaochun
Liang, Xuegang
Guo, Haohui
Ma, Long
Jian, Li
Zhao, Xin
Wang, Jian
Yang, Lvlin
Meng, Zhiqiang
Jin, Qunhua
β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title_full β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title_fullStr β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title_full_unstemmed β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title_short β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
title_sort β2-adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175016/
https://www.ncbi.nlm.nih.gov/pubmed/35794997
http://dx.doi.org/10.1515/med-2022-0498
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