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Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation

The synthesis of secreted cysteine-rich proteins (CRPs) is a long-standing challenge due to protein aggregation and premature formation of inter- and intramolecular disulfide bonds. Chemical synthesis provides reduced CRPs with a higher purity, which is advantageous for folding and isolation. Herein...

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Autores principales: Kumarswamyreddy, Nandarapu, Reddy, Damodara N., Robkis, D. Miklos, Kamiya, Nao, Tsukamoto, Ryoko, Kanaoka, Masahiro M., Higashiyama, Tetsuya, Oishi, Shunsuke, Bode, Jeffrey W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175099/
https://www.ncbi.nlm.nih.gov/pubmed/35755195
http://dx.doi.org/10.1039/d2cb00039c
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author Kumarswamyreddy, Nandarapu
Reddy, Damodara N.
Robkis, D. Miklos
Kamiya, Nao
Tsukamoto, Ryoko
Kanaoka, Masahiro M.
Higashiyama, Tetsuya
Oishi, Shunsuke
Bode, Jeffrey W.
author_facet Kumarswamyreddy, Nandarapu
Reddy, Damodara N.
Robkis, D. Miklos
Kamiya, Nao
Tsukamoto, Ryoko
Kanaoka, Masahiro M.
Higashiyama, Tetsuya
Oishi, Shunsuke
Bode, Jeffrey W.
author_sort Kumarswamyreddy, Nandarapu
collection PubMed
description The synthesis of secreted cysteine-rich proteins (CRPs) is a long-standing challenge due to protein aggregation and premature formation of inter- and intramolecular disulfide bonds. Chemical synthesis provides reduced CRPs with a higher purity, which is advantageous for folding and isolation. Herein, we report the chemical synthesis of pollen tube attractant CRPs Torenia fournieri LURE (TfLURE) and Torenia concolor LURE (TcLURE) and their chimeric analogues via α-ketoacid-hydroxylamine (KAHA) ligation. The bioactivity of chemically synthesized TfLURE protein was shown to be comparable to E. coli expressed recombinant protein through in vitro assay. The convergent protein synthesis approach is beneficial for preparing these small protein variants efficiently.
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spelling pubmed-91750992022-06-23 Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation Kumarswamyreddy, Nandarapu Reddy, Damodara N. Robkis, D. Miklos Kamiya, Nao Tsukamoto, Ryoko Kanaoka, Masahiro M. Higashiyama, Tetsuya Oishi, Shunsuke Bode, Jeffrey W. RSC Chem Biol Chemistry The synthesis of secreted cysteine-rich proteins (CRPs) is a long-standing challenge due to protein aggregation and premature formation of inter- and intramolecular disulfide bonds. Chemical synthesis provides reduced CRPs with a higher purity, which is advantageous for folding and isolation. Herein, we report the chemical synthesis of pollen tube attractant CRPs Torenia fournieri LURE (TfLURE) and Torenia concolor LURE (TcLURE) and their chimeric analogues via α-ketoacid-hydroxylamine (KAHA) ligation. The bioactivity of chemically synthesized TfLURE protein was shown to be comparable to E. coli expressed recombinant protein through in vitro assay. The convergent protein synthesis approach is beneficial for preparing these small protein variants efficiently. RSC 2022-03-18 /pmc/articles/PMC9175099/ /pubmed/35755195 http://dx.doi.org/10.1039/d2cb00039c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Kumarswamyreddy, Nandarapu
Reddy, Damodara N.
Robkis, D. Miklos
Kamiya, Nao
Tsukamoto, Ryoko
Kanaoka, Masahiro M.
Higashiyama, Tetsuya
Oishi, Shunsuke
Bode, Jeffrey W.
Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title_full Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title_fullStr Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title_full_unstemmed Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title_short Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation
title_sort chemical synthesis of torenia plant pollen tube attractant proteins by kaha ligation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175099/
https://www.ncbi.nlm.nih.gov/pubmed/35755195
http://dx.doi.org/10.1039/d2cb00039c
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