Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain move...

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Detalles Bibliográficos
Autores principales: Lewandowski, Tracey M., An, Peng, Ramil, Carlo P., Fang, Ming, Lin, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175107/
https://www.ncbi.nlm.nih.gov/pubmed/35755189
http://dx.doi.org/10.1039/d2cb00107a
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author Lewandowski, Tracey M.
An, Peng
Ramil, Carlo P.
Fang, Ming
Lin, Qing
author_facet Lewandowski, Tracey M.
An, Peng
Ramil, Carlo P.
Fang, Ming
Lin, Qing
author_sort Lewandowski, Tracey M.
collection PubMed
description To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.
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spelling pubmed-91751072022-06-23 Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry Lewandowski, Tracey M. An, Peng Ramil, Carlo P. Fang, Ming Lin, Qing RSC Chem Biol Chemistry To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells. RSC 2022-05-17 /pmc/articles/PMC9175107/ /pubmed/35755189 http://dx.doi.org/10.1039/d2cb00107a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Lewandowski, Tracey M.
An, Peng
Ramil, Carlo P.
Fang, Ming
Lin, Qing
Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title_full Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title_fullStr Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title_full_unstemmed Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title_short Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
title_sort dual fluorescent labeling of glp-1r in live cells via enzymatic tagging and bioorthogonal chemistry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175107/
https://www.ncbi.nlm.nih.gov/pubmed/35755189
http://dx.doi.org/10.1039/d2cb00107a
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