Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain move...

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Detalles Bibliográficos
Autores principales: Lewandowski, Tracey M., An, Peng, Ramil, Carlo P., Fang, Ming, Lin, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175107/
https://www.ncbi.nlm.nih.gov/pubmed/35755189
http://dx.doi.org/10.1039/d2cb00107a

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