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Gene network analysis reveals candidate genes related with the hair follicle development in sheep

BACKGROUND: Merino sheep are the most famous fine wool sheep in the world. They have high wool production and excellent wool quality and have attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibers grown from secondary wool follicles. Therefore, it is n...

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Autores principales: He, Junmin, Zhao, Bingru, Huang, Xixia, Fu, Xuefeng, Liu, Guifen, Tian, Yuezhen, Wu, Cuiling, Mao, Jingyi, Liu, Jing, Gun, Shuangbao, Tian, Kechuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175362/
https://www.ncbi.nlm.nih.gov/pubmed/35672687
http://dx.doi.org/10.1186/s12864-022-08552-2
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author He, Junmin
Zhao, Bingru
Huang, Xixia
Fu, Xuefeng
Liu, Guifen
Tian, Yuezhen
Wu, Cuiling
Mao, Jingyi
Liu, Jing
Gun, Shuangbao
Tian, Kechuan
author_facet He, Junmin
Zhao, Bingru
Huang, Xixia
Fu, Xuefeng
Liu, Guifen
Tian, Yuezhen
Wu, Cuiling
Mao, Jingyi
Liu, Jing
Gun, Shuangbao
Tian, Kechuan
author_sort He, Junmin
collection PubMed
description BACKGROUND: Merino sheep are the most famous fine wool sheep in the world. They have high wool production and excellent wool quality and have attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibers grown from secondary wool follicles. Therefore, it is necessary to study the development of hair follicles to understand the mechanism of wool production. The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. The hair follicle development process is very complex and poorly understood. The purpose of our research is to identify candidate genes related to hair follicle development, provide a theoretical molecular breeding basis for the cultivation of fine wool sheep, and provide a reference for the problems of hair loss and alopecia areata that affect human beings. RESULTS: We analyzed mRNAs data in skin tissues of 18 Merino sheep at four embryonic days (E65, E85, E105 and E135) and two postnatal days (P7 and P30). G1 to G6 represent hair follicles developmental at six stages (i.e. E65 to P30). We identified 7879 differentially expressed genes (DEGs) and 12623 novel DEGs, revealed different expression patterns of these DEGs at six stages of hair follicle development, and demonstrated their complex interactions. DEGs with stage-specific expression were significantly enriched in epidermal differentiation and development, hair follicle development and hair follicle morphogenesis and were enriched in many pathways related to hair follicle development. The key genes (LAMA5, WNT10A, KRT25, SOSTDC1, ZDHHC21, FZD1, BMP7, LRP4, TGFβ2, TMEM79, SOX10, ITGB4, KRT14, ITGA6, and GLI2) affecting hair follicle morphogenesis were identified by network analysis. CONCLUSION: This study provides a new reference for the molecular basis of hair follicle development and lays a foundation for further improving sheep hair follicle breeding. Candidate genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine wool sheep. These results are a valuable resource for biological investigations of fleece evolution in animals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08552-2.
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spelling pubmed-91753622022-06-09 Gene network analysis reveals candidate genes related with the hair follicle development in sheep He, Junmin Zhao, Bingru Huang, Xixia Fu, Xuefeng Liu, Guifen Tian, Yuezhen Wu, Cuiling Mao, Jingyi Liu, Jing Gun, Shuangbao Tian, Kechuan BMC Genomics Research BACKGROUND: Merino sheep are the most famous fine wool sheep in the world. They have high wool production and excellent wool quality and have attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibers grown from secondary wool follicles. Therefore, it is necessary to study the development of hair follicles to understand the mechanism of wool production. The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. The hair follicle development process is very complex and poorly understood. The purpose of our research is to identify candidate genes related to hair follicle development, provide a theoretical molecular breeding basis for the cultivation of fine wool sheep, and provide a reference for the problems of hair loss and alopecia areata that affect human beings. RESULTS: We analyzed mRNAs data in skin tissues of 18 Merino sheep at four embryonic days (E65, E85, E105 and E135) and two postnatal days (P7 and P30). G1 to G6 represent hair follicles developmental at six stages (i.e. E65 to P30). We identified 7879 differentially expressed genes (DEGs) and 12623 novel DEGs, revealed different expression patterns of these DEGs at six stages of hair follicle development, and demonstrated their complex interactions. DEGs with stage-specific expression were significantly enriched in epidermal differentiation and development, hair follicle development and hair follicle morphogenesis and were enriched in many pathways related to hair follicle development. The key genes (LAMA5, WNT10A, KRT25, SOSTDC1, ZDHHC21, FZD1, BMP7, LRP4, TGFβ2, TMEM79, SOX10, ITGB4, KRT14, ITGA6, and GLI2) affecting hair follicle morphogenesis were identified by network analysis. CONCLUSION: This study provides a new reference for the molecular basis of hair follicle development and lays a foundation for further improving sheep hair follicle breeding. Candidate genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine wool sheep. These results are a valuable resource for biological investigations of fleece evolution in animals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08552-2. BioMed Central 2022-06-08 /pmc/articles/PMC9175362/ /pubmed/35672687 http://dx.doi.org/10.1186/s12864-022-08552-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
He, Junmin
Zhao, Bingru
Huang, Xixia
Fu, Xuefeng
Liu, Guifen
Tian, Yuezhen
Wu, Cuiling
Mao, Jingyi
Liu, Jing
Gun, Shuangbao
Tian, Kechuan
Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title_full Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title_fullStr Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title_full_unstemmed Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title_short Gene network analysis reveals candidate genes related with the hair follicle development in sheep
title_sort gene network analysis reveals candidate genes related with the hair follicle development in sheep
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175362/
https://www.ncbi.nlm.nih.gov/pubmed/35672687
http://dx.doi.org/10.1186/s12864-022-08552-2
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