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Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid

The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnet...

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Autores principales: Alexandre, Lucile, Bendali, Amel, Pereiro, Iago, Azimani, Madad, Dumas, Simon, Malaquin, Laurent, Mai, Thanh Duc, Descroix, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176165/
https://www.ncbi.nlm.nih.gov/pubmed/35676309
http://dx.doi.org/10.1038/s41598-022-13304-z
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author Alexandre, Lucile
Bendali, Amel
Pereiro, Iago
Azimani, Madad
Dumas, Simon
Malaquin, Laurent
Mai, Thanh Duc
Descroix, Stéphanie
author_facet Alexandre, Lucile
Bendali, Amel
Pereiro, Iago
Azimani, Madad
Dumas, Simon
Malaquin, Laurent
Mai, Thanh Duc
Descroix, Stéphanie
author_sort Alexandre, Lucile
collection PubMed
description The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhancing mass transport, reduce non-specific binding and multiply the detection signal threefold by transitioning between packed and fluidization states. With the integration of a full ELISA protocol in a single microfluidic chamber, we show a twofold reduction in LOD compared to conventional methods along with a large dynamic range (10 pg/mL to 2 ng/mL). We additionally demonstrate its application to IL-6 detection in undiluted serum samples.
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spelling pubmed-91761652022-06-09 Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid Alexandre, Lucile Bendali, Amel Pereiro, Iago Azimani, Madad Dumas, Simon Malaquin, Laurent Mai, Thanh Duc Descroix, Stéphanie Sci Rep Article The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhancing mass transport, reduce non-specific binding and multiply the detection signal threefold by transitioning between packed and fluidization states. With the integration of a full ELISA protocol in a single microfluidic chamber, we show a twofold reduction in LOD compared to conventional methods along with a large dynamic range (10 pg/mL to 2 ng/mL). We additionally demonstrate its application to IL-6 detection in undiluted serum samples. Nature Publishing Group UK 2022-06-08 /pmc/articles/PMC9176165/ /pubmed/35676309 http://dx.doi.org/10.1038/s41598-022-13304-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Alexandre, Lucile
Bendali, Amel
Pereiro, Iago
Azimani, Madad
Dumas, Simon
Malaquin, Laurent
Mai, Thanh Duc
Descroix, Stéphanie
Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title_full Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title_fullStr Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title_full_unstemmed Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title_short Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid
title_sort modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker il-6 in biofluid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176165/
https://www.ncbi.nlm.nih.gov/pubmed/35676309
http://dx.doi.org/10.1038/s41598-022-13304-z
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