Cargando…

An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19

The emergence of the COVID-19 epidemic has affected the lives of hundreds of millions of people globally. There is no doubt that the development of fast and sensitive detection methods is crucial while the worldwide effective vaccination programs are miles away from actualization. In this study, we...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Mengdi, Zhang, Xiaohui, Zhang, Xin, Zahra, Qurat ul ain, Huang, Zenghui, Chen, Ying, Song, Chunxia, Song, Min, Jiang, Hongjuan, Luo, Zhaofeng, Lu, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176179/
https://www.ncbi.nlm.nih.gov/pubmed/35716641
http://dx.doi.org/10.1016/j.bios.2022.114436
_version_ 1784722609060970496
author Yu, Mengdi
Zhang, Xiaohui
Zhang, Xin
Zahra, Qurat ul ain
Huang, Zenghui
Chen, Ying
Song, Chunxia
Song, Min
Jiang, Hongjuan
Luo, Zhaofeng
Lu, Ying
author_facet Yu, Mengdi
Zhang, Xiaohui
Zhang, Xin
Zahra, Qurat ul ain
Huang, Zenghui
Chen, Ying
Song, Chunxia
Song, Min
Jiang, Hongjuan
Luo, Zhaofeng
Lu, Ying
author_sort Yu, Mengdi
collection PubMed
description The emergence of the COVID-19 epidemic has affected the lives of hundreds of millions of people globally. There is no doubt that the development of fast and sensitive detection methods is crucial while the worldwide effective vaccination programs are miles away from actualization. In this study, we have reported an electrochemical N protein aptamer sensor with complementary oligonucleotide as probe for the specific detection of COVID-19. The electrochemical aptasensor was prepared by fixing the double-stranded DNA hybrid obtained by the hybridization of N protein aptamer and its Fc-labeled complementary strand on the surface of a gold electrode. After incubation with the target, the aptamer dissociated from the labeled complementary DNA oligonucleotide hybrid to preferentially bind with N protein in the solution. The concentration of N protein was measured by detecting the changes in electrochemical current signals induced by the conformational transformation of the complementary DNA oligonucleotide left on the electrode surface. The sensor had a linear relationship between the logarithm of the N protein concentration from 10 fM to 100 nM (ΔI(p) = 0.098 log C(N protein)/fM - 0.08433, R(2) = 0.99), and the detection limitation was 1 fM (S/N = 3). The electrochemical aptamer sensor was applied to test the spiked concentrations of throat swabs and blood samples from three volunteers, and the obtained results proved that the sensor has great potentials for the early detection of COVID-19 in patients.
format Online
Article
Text
id pubmed-9176179
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-91761792022-06-09 An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19 Yu, Mengdi Zhang, Xiaohui Zhang, Xin Zahra, Qurat ul ain Huang, Zenghui Chen, Ying Song, Chunxia Song, Min Jiang, Hongjuan Luo, Zhaofeng Lu, Ying Biosens Bioelectron Article The emergence of the COVID-19 epidemic has affected the lives of hundreds of millions of people globally. There is no doubt that the development of fast and sensitive detection methods is crucial while the worldwide effective vaccination programs are miles away from actualization. In this study, we have reported an electrochemical N protein aptamer sensor with complementary oligonucleotide as probe for the specific detection of COVID-19. The electrochemical aptasensor was prepared by fixing the double-stranded DNA hybrid obtained by the hybridization of N protein aptamer and its Fc-labeled complementary strand on the surface of a gold electrode. After incubation with the target, the aptamer dissociated from the labeled complementary DNA oligonucleotide hybrid to preferentially bind with N protein in the solution. The concentration of N protein was measured by detecting the changes in electrochemical current signals induced by the conformational transformation of the complementary DNA oligonucleotide left on the electrode surface. The sensor had a linear relationship between the logarithm of the N protein concentration from 10 fM to 100 nM (ΔI(p) = 0.098 log C(N protein)/fM - 0.08433, R(2) = 0.99), and the detection limitation was 1 fM (S/N = 3). The electrochemical aptamer sensor was applied to test the spiked concentrations of throat swabs and blood samples from three volunteers, and the obtained results proved that the sensor has great potentials for the early detection of COVID-19 in patients. Elsevier B.V. 2022-10-01 2022-06-08 /pmc/articles/PMC9176179/ /pubmed/35716641 http://dx.doi.org/10.1016/j.bios.2022.114436 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Yu, Mengdi
Zhang, Xiaohui
Zhang, Xin
Zahra, Qurat ul ain
Huang, Zenghui
Chen, Ying
Song, Chunxia
Song, Min
Jiang, Hongjuan
Luo, Zhaofeng
Lu, Ying
An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title_full An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title_fullStr An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title_full_unstemmed An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title_short An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
title_sort electrochemical aptasensor with n protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of covid-19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176179/
https://www.ncbi.nlm.nih.gov/pubmed/35716641
http://dx.doi.org/10.1016/j.bios.2022.114436
work_keys_str_mv AT yumengdi anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zhangxiaohui anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zhangxin anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zahraquratulain anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT huangzenghui anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT chenying anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT songchunxia anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT songmin anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT jianghongjuan anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT luozhaofeng anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT luying anelectrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT yumengdi electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zhangxiaohui electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zhangxin electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT zahraquratulain electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT huangzenghui electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT chenying electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT songchunxia electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT songmin electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT jianghongjuan electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT luozhaofeng electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19
AT luying electrochemicalaptasensorwithnproteinbindingaptamercomplementaryoligonucleotideasprobeforultrasensitivedetectionofcovid19