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Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals

Increased bacterial translocation in the gut and bloodstream infections are both major comorbidities of heart failure and myocardial infarction (MI). However, the alterations in the microbiome of the blood of patients with MI remain unclear. To test this hypothesis, we conducted this case-control st...

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Autores principales: Khan, Ikram, Khan, Imran, Kakakhel, Mian Adnan, Xiaowei, Zhang, Ting, Mao, Ali, Ikram, Fei, Yu, Jianye, Zhou, Zhiqiang, Li, Lizhe, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176212/
https://www.ncbi.nlm.nih.gov/pubmed/35694288
http://dx.doi.org/10.3389/fmicb.2022.845038
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author Khan, Ikram
Khan, Imran
Kakakhel, Mian Adnan
Xiaowei, Zhang
Ting, Mao
Ali, Ikram
Fei, Yu
Jianye, Zhou
Zhiqiang, Li
Lizhe, An
author_facet Khan, Ikram
Khan, Imran
Kakakhel, Mian Adnan
Xiaowei, Zhang
Ting, Mao
Ali, Ikram
Fei, Yu
Jianye, Zhou
Zhiqiang, Li
Lizhe, An
author_sort Khan, Ikram
collection PubMed
description Increased bacterial translocation in the gut and bloodstream infections are both major comorbidities of heart failure and myocardial infarction (MI). However, the alterations in the microbiome of the blood of patients with MI remain unclear. To test this hypothesis, we conducted this case-control study to explore the microbiota compositions in the blood of Chinese patients with MI. Using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of the 16S ribosomal RNA (rRNA) gene, the microbiota communities in the blood of 29 patients with MI and 29 healthy controls were examined. In addition, the relationship between the blood microbiome and clinical features of MI was investigated. This study revealed a significant reduction in alpha diversity (Shannon index) in the MI group compared with the healthy controls. Also, a significant difference was detected in the structure and richness between the patients with MI and healthy controls. The members of the phylum Actinobacteria, class Actinobacteria, order Bifdobacteriales, family Bifidobacteriaceae, and genus Bifidobacterium were significantly abundant in the MI group, while the members of the phylum Bacteroidetes, class Bacteroidia, and order Bacteroidales were significantly enriched in the healthy controls (p < 0.05). Moreover, the functional analysis revealed a significant variation between both groups. For instance, the enrichment of genes involved in the metabolism pathways of three amino acids decreased, that is, nucleotide transport and metabolism, coenzyme transport and metabolism, and lipid transport and metabolism, among others. Our study will contribute to a better knowledge of the microbiota of blood, which will further lead to improved MI diagnosis and therapy. Further study is needed to determine the role of the blood microbiota in human health and disease.
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spelling pubmed-91762122022-06-09 Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals Khan, Ikram Khan, Imran Kakakhel, Mian Adnan Xiaowei, Zhang Ting, Mao Ali, Ikram Fei, Yu Jianye, Zhou Zhiqiang, Li Lizhe, An Front Microbiol Microbiology Increased bacterial translocation in the gut and bloodstream infections are both major comorbidities of heart failure and myocardial infarction (MI). However, the alterations in the microbiome of the blood of patients with MI remain unclear. To test this hypothesis, we conducted this case-control study to explore the microbiota compositions in the blood of Chinese patients with MI. Using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of the 16S ribosomal RNA (rRNA) gene, the microbiota communities in the blood of 29 patients with MI and 29 healthy controls were examined. In addition, the relationship between the blood microbiome and clinical features of MI was investigated. This study revealed a significant reduction in alpha diversity (Shannon index) in the MI group compared with the healthy controls. Also, a significant difference was detected in the structure and richness between the patients with MI and healthy controls. The members of the phylum Actinobacteria, class Actinobacteria, order Bifdobacteriales, family Bifidobacteriaceae, and genus Bifidobacterium were significantly abundant in the MI group, while the members of the phylum Bacteroidetes, class Bacteroidia, and order Bacteroidales were significantly enriched in the healthy controls (p < 0.05). Moreover, the functional analysis revealed a significant variation between both groups. For instance, the enrichment of genes involved in the metabolism pathways of three amino acids decreased, that is, nucleotide transport and metabolism, coenzyme transport and metabolism, and lipid transport and metabolism, among others. Our study will contribute to a better knowledge of the microbiota of blood, which will further lead to improved MI diagnosis and therapy. Further study is needed to determine the role of the blood microbiota in human health and disease. Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9176212/ /pubmed/35694288 http://dx.doi.org/10.3389/fmicb.2022.845038 Text en Copyright © 2022 Khan, Khan, Kakakhel, Xiaowei, Ting, Ali, Fei, Jianye, Zhiqiang and Lizhe. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Khan, Ikram
Khan, Imran
Kakakhel, Mian Adnan
Xiaowei, Zhang
Ting, Mao
Ali, Ikram
Fei, Yu
Jianye, Zhou
Zhiqiang, Li
Lizhe, An
Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title_full Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title_fullStr Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title_full_unstemmed Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title_short Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals
title_sort comparison of microbial populations in the blood of patients with myocardial infarction and healthy individuals
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176212/
https://www.ncbi.nlm.nih.gov/pubmed/35694288
http://dx.doi.org/10.3389/fmicb.2022.845038
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