Cargando…

Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress

Dehydration-responsive element-binding proteins (DREBs) belong to members of the AP2/ERF transcription factor superfamily, which has been reported to involve various abiotic-stress responses and tolerance in plants. However, research on the DREB-family is still limited in alfalfa (Medicago sativa L....

Descripción completa

Detalles Bibliográficos
Autores principales: Sheng, Song, Guo, Xinyu, Wu, Changzheng, Xiang, Yucheng, Duan, Shuhui, Yang, Weiqin, Le, Wenrui, Cao, Fengchun, Liu, Laihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176237/
https://www.ncbi.nlm.nih.gov/pubmed/35642507
http://dx.doi.org/10.1080/15592324.2022.2081420
_version_ 1784722620891004928
author Sheng, Song
Guo, Xinyu
Wu, Changzheng
Xiang, Yucheng
Duan, Shuhui
Yang, Weiqin
Le, Wenrui
Cao, Fengchun
Liu, Laihua
author_facet Sheng, Song
Guo, Xinyu
Wu, Changzheng
Xiang, Yucheng
Duan, Shuhui
Yang, Weiqin
Le, Wenrui
Cao, Fengchun
Liu, Laihua
author_sort Sheng, Song
collection PubMed
description Dehydration-responsive element-binding proteins (DREBs) belong to members of the AP2/ERF transcription factor superfamily, which has been reported to involve various abiotic-stress responses and tolerance in plants. However, research on the DREB-family is still limited in alfalfa (Medicago sativa L.), a forage legume cultivated worldwide. The recent genome-sequence release of the alfalfa cultivar “XinJiangDaYe” allowed us to identify 172 DREBs by a multi-step homolog search. The phylogenetic analysis indicated that such MsDREBs could be classified into 5 groups, namely A-1 (56 members), A-2 (39), A-3 (3), A-4 (61) and 13 (A-5 (13), thus adding substantial new members to the DREB-family in alfalfa. Furthermore, a comprehensive survey in silico of conserved motif, gene structure, molecular weight, and isoelectric point (pI) as well as gene expression was conducted. The resulting data showed that, for cold-stress response, 33 differentially expressed MsDREBs were identified with a threshold of Log2-fold > 1, and most of which were transcriptionally upregulated within 48 h during a cold treatment(s). Moreover, the expression profiling of MsDREBs from two ecotypes of alfalfa subspecies i.e. M. sativa ssp. falcata (F56, from a colder region of Central Asia) and M. sativa ssp. sativa (B47, from Near East) revealed that most of the cold-stress responsive MsDREBs exhibited a significantly lower expression in F56, leading to a proposal of the existence of a distinct mechanism(s) for cold tolerance regulated by DREB-related action, which would have been evolved in alfalfa with a genotypic specificity. Additionally, by examining the transcriptome of a freezing-tolerance species (M. sativa cv. Zhaodong), eight DREBs were found to be implicated in a long-term freezing-stress adaptation with a great potential. Taken together, the current genome-wide identification in alfalfa points to the importance of some MsDREBs in the cold-stress response, providing some promising molecular targets to be functionally characterized for the improvement of cold tolerance in crops including alfalfa.
format Online
Article
Text
id pubmed-9176237
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-91762372022-06-09 Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress Sheng, Song Guo, Xinyu Wu, Changzheng Xiang, Yucheng Duan, Shuhui Yang, Weiqin Le, Wenrui Cao, Fengchun Liu, Laihua Plant Signal Behav Short Communication Dehydration-responsive element-binding proteins (DREBs) belong to members of the AP2/ERF transcription factor superfamily, which has been reported to involve various abiotic-stress responses and tolerance in plants. However, research on the DREB-family is still limited in alfalfa (Medicago sativa L.), a forage legume cultivated worldwide. The recent genome-sequence release of the alfalfa cultivar “XinJiangDaYe” allowed us to identify 172 DREBs by a multi-step homolog search. The phylogenetic analysis indicated that such MsDREBs could be classified into 5 groups, namely A-1 (56 members), A-2 (39), A-3 (3), A-4 (61) and 13 (A-5 (13), thus adding substantial new members to the DREB-family in alfalfa. Furthermore, a comprehensive survey in silico of conserved motif, gene structure, molecular weight, and isoelectric point (pI) as well as gene expression was conducted. The resulting data showed that, for cold-stress response, 33 differentially expressed MsDREBs were identified with a threshold of Log2-fold > 1, and most of which were transcriptionally upregulated within 48 h during a cold treatment(s). Moreover, the expression profiling of MsDREBs from two ecotypes of alfalfa subspecies i.e. M. sativa ssp. falcata (F56, from a colder region of Central Asia) and M. sativa ssp. sativa (B47, from Near East) revealed that most of the cold-stress responsive MsDREBs exhibited a significantly lower expression in F56, leading to a proposal of the existence of a distinct mechanism(s) for cold tolerance regulated by DREB-related action, which would have been evolved in alfalfa with a genotypic specificity. Additionally, by examining the transcriptome of a freezing-tolerance species (M. sativa cv. Zhaodong), eight DREBs were found to be implicated in a long-term freezing-stress adaptation with a great potential. Taken together, the current genome-wide identification in alfalfa points to the importance of some MsDREBs in the cold-stress response, providing some promising molecular targets to be functionally characterized for the improvement of cold tolerance in crops including alfalfa. Taylor & Francis 2022-06-01 /pmc/articles/PMC9176237/ /pubmed/35642507 http://dx.doi.org/10.1080/15592324.2022.2081420 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Sheng, Song
Guo, Xinyu
Wu, Changzheng
Xiang, Yucheng
Duan, Shuhui
Yang, Weiqin
Le, Wenrui
Cao, Fengchun
Liu, Laihua
Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title_full Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title_fullStr Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title_full_unstemmed Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title_short Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
title_sort genome-wide identification and expression analysis of dreb genes in alfalfa (medicago sativa) in response to cold stress
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176237/
https://www.ncbi.nlm.nih.gov/pubmed/35642507
http://dx.doi.org/10.1080/15592324.2022.2081420
work_keys_str_mv AT shengsong genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT guoxinyu genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT wuchangzheng genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT xiangyucheng genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT duanshuhui genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT yangweiqin genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT lewenrui genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT caofengchun genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress
AT liulaihua genomewideidentificationandexpressionanalysisofdrebgenesinalfalfamedicagosativainresponsetocoldstress