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Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales

The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dino...

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Autores principales: Tomasch, Jürgen, Ringel, Victoria, Wang, Hui, Freese, Heike M., Bartling, Pascal, Brinkmann, Henner, Vollmers, John, Jarek, Michael, Wagner-Döbler, Irene, Petersen, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176285/
https://www.ncbi.nlm.nih.gov/pubmed/35254236
http://dx.doi.org/10.1099/mgen.0.000787
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author Tomasch, Jürgen
Ringel, Victoria
Wang, Hui
Freese, Heike M.
Bartling, Pascal
Brinkmann, Henner
Vollmers, John
Jarek, Michael
Wagner-Döbler, Irene
Petersen, Jörn
author_facet Tomasch, Jürgen
Ringel, Victoria
Wang, Hui
Freese, Heike M.
Bartling, Pascal
Brinkmann, Henner
Vollmers, John
Jarek, Michael
Wagner-Döbler, Irene
Petersen, Jörn
author_sort Tomasch, Jürgen
collection PubMed
description The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae . Here, we show that two large natural RepABC-type plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co-cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co-culture.
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spelling pubmed-91762852022-06-09 Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales Tomasch, Jürgen Ringel, Victoria Wang, Hui Freese, Heike M. Bartling, Pascal Brinkmann, Henner Vollmers, John Jarek, Michael Wagner-Döbler, Irene Petersen, Jörn Microb Genom Research Articles The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae . Here, we show that two large natural RepABC-type plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co-cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co-culture. Microbiology Society 2022-03-07 /pmc/articles/PMC9176285/ /pubmed/35254236 http://dx.doi.org/10.1099/mgen.0.000787 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Research Articles
Tomasch, Jürgen
Ringel, Victoria
Wang, Hui
Freese, Heike M.
Bartling, Pascal
Brinkmann, Henner
Vollmers, John
Jarek, Michael
Wagner-Döbler, Irene
Petersen, Jörn
Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title_full Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title_fullStr Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title_full_unstemmed Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title_short Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
title_sort fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine rhodobacterales
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176285/
https://www.ncbi.nlm.nih.gov/pubmed/35254236
http://dx.doi.org/10.1099/mgen.0.000787
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