Cargando…

Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover

A thermophilic Geobacillus bacterial strain, WSUCF1 contains different carbohydrate-active enzymes (CAZymes) capable of hydrolyzing hemicellulose in lignocellulosic biomass. We used proteomic, genomic, and bioinformatic tools, and genomic data to analyze the relative abundance of cellulolytic, hemic...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhalla, Aditya, Arce, Jessie, Ubanwa, Bryan, Singh, Gursharan, Sani, Rajesh K., Balan, Venkatesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176393/
https://www.ncbi.nlm.nih.gov/pubmed/35694290
http://dx.doi.org/10.3389/fmicb.2022.844287
_version_ 1784722655185731584
author Bhalla, Aditya
Arce, Jessie
Ubanwa, Bryan
Singh, Gursharan
Sani, Rajesh K.
Balan, Venkatesh
author_facet Bhalla, Aditya
Arce, Jessie
Ubanwa, Bryan
Singh, Gursharan
Sani, Rajesh K.
Balan, Venkatesh
author_sort Bhalla, Aditya
collection PubMed
description A thermophilic Geobacillus bacterial strain, WSUCF1 contains different carbohydrate-active enzymes (CAZymes) capable of hydrolyzing hemicellulose in lignocellulosic biomass. We used proteomic, genomic, and bioinformatic tools, and genomic data to analyze the relative abundance of cellulolytic, hemicellulolytic, and lignin modifying enzymes present in the secretomes. Results showed that CAZyme profiles of secretomes varied based on the substrate type and complexity, composition, and pretreatment conditions. The enzyme activity of secretomes also changed depending on the substrate used. The secretomes were used in combination with commercial and purified enzymes to carry out saccharification of ammonia fiber expansion (AFEX)-pretreated corn stover and extractive ammonia (EA)-pretreated corn stover. When WSUCF1 bacterial secretome produced at different conditions was combined with a small percentage of commercial enzymes, we observed efficient saccharification of EA-CS, and the results were comparable to using a commercial enzyme cocktail (87% glucan and 70% xylan conversion). It also opens the possibility of producing CAZymes in a biorefinery using inexpensive substrates, such as AFEX-pretreated corn stover and Avicel, and eliminates expensive enzyme processing steps that are used in enzyme manufacturing. Implementing in-house enzyme production is expected to significantly reduce the cost of enzymes and biofuel processing cost.
format Online
Article
Text
id pubmed-9176393
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91763932022-06-09 Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover Bhalla, Aditya Arce, Jessie Ubanwa, Bryan Singh, Gursharan Sani, Rajesh K. Balan, Venkatesh Front Microbiol Microbiology A thermophilic Geobacillus bacterial strain, WSUCF1 contains different carbohydrate-active enzymes (CAZymes) capable of hydrolyzing hemicellulose in lignocellulosic biomass. We used proteomic, genomic, and bioinformatic tools, and genomic data to analyze the relative abundance of cellulolytic, hemicellulolytic, and lignin modifying enzymes present in the secretomes. Results showed that CAZyme profiles of secretomes varied based on the substrate type and complexity, composition, and pretreatment conditions. The enzyme activity of secretomes also changed depending on the substrate used. The secretomes were used in combination with commercial and purified enzymes to carry out saccharification of ammonia fiber expansion (AFEX)-pretreated corn stover and extractive ammonia (EA)-pretreated corn stover. When WSUCF1 bacterial secretome produced at different conditions was combined with a small percentage of commercial enzymes, we observed efficient saccharification of EA-CS, and the results were comparable to using a commercial enzyme cocktail (87% glucan and 70% xylan conversion). It also opens the possibility of producing CAZymes in a biorefinery using inexpensive substrates, such as AFEX-pretreated corn stover and Avicel, and eliminates expensive enzyme processing steps that are used in enzyme manufacturing. Implementing in-house enzyme production is expected to significantly reduce the cost of enzymes and biofuel processing cost. Frontiers Media S.A. 2022-05-25 /pmc/articles/PMC9176393/ /pubmed/35694290 http://dx.doi.org/10.3389/fmicb.2022.844287 Text en Copyright © 2022 Bhalla, Arce, Ubanwa, Singh, Sani and Balan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bhalla, Aditya
Arce, Jessie
Ubanwa, Bryan
Singh, Gursharan
Sani, Rajesh K.
Balan, Venkatesh
Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title_full Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title_fullStr Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title_full_unstemmed Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title_short Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover
title_sort thermophilic geobacillus wsucf1 secretome for saccharification of ammonia fiber expansion and extractive ammonia pretreated corn stover
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176393/
https://www.ncbi.nlm.nih.gov/pubmed/35694290
http://dx.doi.org/10.3389/fmicb.2022.844287
work_keys_str_mv AT bhallaaditya thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover
AT arcejessie thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover
AT ubanwabryan thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover
AT singhgursharan thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover
AT sanirajeshk thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover
AT balanvenkatesh thermophilicgeobacilluswsucf1secretomeforsaccharificationofammoniafiberexpansionandextractiveammoniapretreatedcornstover