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Myostain is involved in ginsenoside Rb1-mediated anti-obesity
CONTEXT: Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of Panax ginseng C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176416/ https://www.ncbi.nlm.nih.gov/pubmed/35639355 http://dx.doi.org/10.1080/13880209.2022.2074056 |
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author | Li, Hong-Shi Kuang, Jiang-Ying Liu, Gui-Jun Wu, Wei-Jie Yin, Xian-Lun Li, Hao-Dong Wang, Lei Qin, Tao Zhang, Wen-Cheng Sun, Yuan-Yuan |
author_facet | Li, Hong-Shi Kuang, Jiang-Ying Liu, Gui-Jun Wu, Wei-Jie Yin, Xian-Lun Li, Hao-Dong Wang, Lei Qin, Tao Zhang, Wen-Cheng Sun, Yuan-Yuan |
author_sort | Li, Hong-Shi |
collection | PubMed |
description | CONTEXT: Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of Panax ginseng C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored. OBJECTIVE: This paper intends to further explore the mechanism of Rb1 in regulating obesity. MATERIALS AND METHODS: The C57BL/6 obese mice were divided into two groups: the control (CTR) and Rb1. The CTR group [intraperitoneally (ip) administered with saline] and the Rb1 group (ip administered with Rb1, 40 mg/kg/d) were treated daily for four weeks. In vitro, Rb1 (0, 10, 20, 40 μM) was added to differentiated C2C12 cells and Rb1 (0, 20, 40 μM) was added to 3T3-L1 cells. After 24 h, total RNA and protein from C2C12 cells and 3T3-L1 cells were used to detect myostatin (MSTN) and fibronectin type III domain-containing 5 (FNDC5) expression. RESULTS: Rb1 reduced the body weight and adipocyte size. Improved glucose tolerance and increased basic metabolic activity were also found in Rb1 treated mice. MSTN was downregulated in differentiated C2C12 cells, 3T3-L1 cells and adipose tissues upon Rb1 treatment. FNDC5 was increased after Rb1 treatment. However, MSTN overexpression attenuated Rb1-mediated decrease accumulation of lipid droplets in differentiated 3T3-L1 adipocytes. DISCUSSION & CONCLUSIONS: Rb1 may ameliorate obesity in part through the MSTN/FNDC5 signalling pathway. Our results showed that Rb1 can be used as an effective drug in the treatment of human obesity. |
format | Online Article Text |
id | pubmed-9176416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-91764162022-06-09 Myostain is involved in ginsenoside Rb1-mediated anti-obesity Li, Hong-Shi Kuang, Jiang-Ying Liu, Gui-Jun Wu, Wei-Jie Yin, Xian-Lun Li, Hao-Dong Wang, Lei Qin, Tao Zhang, Wen-Cheng Sun, Yuan-Yuan Pharm Biol Research Article CONTEXT: Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of Panax ginseng C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored. OBJECTIVE: This paper intends to further explore the mechanism of Rb1 in regulating obesity. MATERIALS AND METHODS: The C57BL/6 obese mice were divided into two groups: the control (CTR) and Rb1. The CTR group [intraperitoneally (ip) administered with saline] and the Rb1 group (ip administered with Rb1, 40 mg/kg/d) were treated daily for four weeks. In vitro, Rb1 (0, 10, 20, 40 μM) was added to differentiated C2C12 cells and Rb1 (0, 20, 40 μM) was added to 3T3-L1 cells. After 24 h, total RNA and protein from C2C12 cells and 3T3-L1 cells were used to detect myostatin (MSTN) and fibronectin type III domain-containing 5 (FNDC5) expression. RESULTS: Rb1 reduced the body weight and adipocyte size. Improved glucose tolerance and increased basic metabolic activity were also found in Rb1 treated mice. MSTN was downregulated in differentiated C2C12 cells, 3T3-L1 cells and adipose tissues upon Rb1 treatment. FNDC5 was increased after Rb1 treatment. However, MSTN overexpression attenuated Rb1-mediated decrease accumulation of lipid droplets in differentiated 3T3-L1 adipocytes. DISCUSSION & CONCLUSIONS: Rb1 may ameliorate obesity in part through the MSTN/FNDC5 signalling pathway. Our results showed that Rb1 can be used as an effective drug in the treatment of human obesity. Taylor & Francis 2022-05-31 /pmc/articles/PMC9176416/ /pubmed/35639355 http://dx.doi.org/10.1080/13880209.2022.2074056 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Hong-Shi Kuang, Jiang-Ying Liu, Gui-Jun Wu, Wei-Jie Yin, Xian-Lun Li, Hao-Dong Wang, Lei Qin, Tao Zhang, Wen-Cheng Sun, Yuan-Yuan Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title | Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title_full | Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title_fullStr | Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title_full_unstemmed | Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title_short | Myostain is involved in ginsenoside Rb1-mediated anti-obesity |
title_sort | myostain is involved in ginsenoside rb1-mediated anti-obesity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176416/ https://www.ncbi.nlm.nih.gov/pubmed/35639355 http://dx.doi.org/10.1080/13880209.2022.2074056 |
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