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Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds

A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the react...

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Autores principales: Pereira, Sarah A. P., Baptista L, A. Catarina, Biancalana, Lorenzo, Marchetti, Fabio, Dyson, Paul J., Saraiva, M. Lúcia M. F. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176637/
https://www.ncbi.nlm.nih.gov/pubmed/35635138
http://dx.doi.org/10.1080/14756366.2022.2073443
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author Pereira, Sarah A. P.
Baptista L, A. Catarina
Biancalana, Lorenzo
Marchetti, Fabio
Dyson, Paul J.
Saraiva, M. Lúcia M. F. S.
author_facet Pereira, Sarah A. P.
Baptista L, A. Catarina
Biancalana, Lorenzo
Marchetti, Fabio
Dyson, Paul J.
Saraiva, M. Lúcia M. F. S.
author_sort Pereira, Sarah A. P.
collection PubMed
description A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the reaction of L-glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of GST P1-1 to afford the GS-DNB conjugate and the reaction may be monitored by an increase in absorbance at 340 nm. A series of ruthenium, iron, osmium and iridium complexes were evaluated as GST P1-1 inhibitors by evaluating their half-maximal inhibitory concentration (IC(50)). An iridium compound displays the lowest IC(50) value of 6.7 ± 0.7 µM and an iron compound displays the highest IC(50) value of 275 ± 9 µM. The SIA method is simple to use, robust, reliable, and efficient and uses fewer reagents than batch methods and each analysis takes only 5 minutes.
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spelling pubmed-91766372022-06-09 Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds Pereira, Sarah A. P. Baptista L, A. Catarina Biancalana, Lorenzo Marchetti, Fabio Dyson, Paul J. Saraiva, M. Lúcia M. F. S. J Enzyme Inhib Med Chem Research Papers A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the reaction of L-glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of GST P1-1 to afford the GS-DNB conjugate and the reaction may be monitored by an increase in absorbance at 340 nm. A series of ruthenium, iron, osmium and iridium complexes were evaluated as GST P1-1 inhibitors by evaluating their half-maximal inhibitory concentration (IC(50)). An iridium compound displays the lowest IC(50) value of 6.7 ± 0.7 µM and an iron compound displays the highest IC(50) value of 275 ± 9 µM. The SIA method is simple to use, robust, reliable, and efficient and uses fewer reagents than batch methods and each analysis takes only 5 minutes. Taylor & Francis 2022-05-29 /pmc/articles/PMC9176637/ /pubmed/35635138 http://dx.doi.org/10.1080/14756366.2022.2073443 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Pereira, Sarah A. P.
Baptista L, A. Catarina
Biancalana, Lorenzo
Marchetti, Fabio
Dyson, Paul J.
Saraiva, M. Lúcia M. F. S.
Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title_full Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title_fullStr Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title_full_unstemmed Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title_short Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
title_sort automated approach for the evaluation of glutathione-s-transferase p1-1 inhibition by organometallic anticancer compounds
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176637/
https://www.ncbi.nlm.nih.gov/pubmed/35635138
http://dx.doi.org/10.1080/14756366.2022.2073443
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