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Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds
A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the react...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176637/ https://www.ncbi.nlm.nih.gov/pubmed/35635138 http://dx.doi.org/10.1080/14756366.2022.2073443 |
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author | Pereira, Sarah A. P. Baptista L, A. Catarina Biancalana, Lorenzo Marchetti, Fabio Dyson, Paul J. Saraiva, M. Lúcia M. F. S. |
author_facet | Pereira, Sarah A. P. Baptista L, A. Catarina Biancalana, Lorenzo Marchetti, Fabio Dyson, Paul J. Saraiva, M. Lúcia M. F. S. |
author_sort | Pereira, Sarah A. P. |
collection | PubMed |
description | A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the reaction of L-glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of GST P1-1 to afford the GS-DNB conjugate and the reaction may be monitored by an increase in absorbance at 340 nm. A series of ruthenium, iron, osmium and iridium complexes were evaluated as GST P1-1 inhibitors by evaluating their half-maximal inhibitory concentration (IC(50)). An iridium compound displays the lowest IC(50) value of 6.7 ± 0.7 µM and an iron compound displays the highest IC(50) value of 275 ± 9 µM. The SIA method is simple to use, robust, reliable, and efficient and uses fewer reagents than batch methods and each analysis takes only 5 minutes. |
format | Online Article Text |
id | pubmed-9176637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-91766372022-06-09 Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds Pereira, Sarah A. P. Baptista L, A. Catarina Biancalana, Lorenzo Marchetti, Fabio Dyson, Paul J. Saraiva, M. Lúcia M. F. S. J Enzyme Inhib Med Chem Research Papers A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the reaction of L-glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of GST P1-1 to afford the GS-DNB conjugate and the reaction may be monitored by an increase in absorbance at 340 nm. A series of ruthenium, iron, osmium and iridium complexes were evaluated as GST P1-1 inhibitors by evaluating their half-maximal inhibitory concentration (IC(50)). An iridium compound displays the lowest IC(50) value of 6.7 ± 0.7 µM and an iron compound displays the highest IC(50) value of 275 ± 9 µM. The SIA method is simple to use, robust, reliable, and efficient and uses fewer reagents than batch methods and each analysis takes only 5 minutes. Taylor & Francis 2022-05-29 /pmc/articles/PMC9176637/ /pubmed/35635138 http://dx.doi.org/10.1080/14756366.2022.2073443 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Pereira, Sarah A. P. Baptista L, A. Catarina Biancalana, Lorenzo Marchetti, Fabio Dyson, Paul J. Saraiva, M. Lúcia M. F. S. Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title | Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title_full | Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title_fullStr | Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title_full_unstemmed | Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title_short | Automated approach for the evaluation of glutathione-S-transferase P1-1 inhibition by organometallic anticancer compounds |
title_sort | automated approach for the evaluation of glutathione-s-transferase p1-1 inhibition by organometallic anticancer compounds |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176637/ https://www.ncbi.nlm.nih.gov/pubmed/35635138 http://dx.doi.org/10.1080/14756366.2022.2073443 |
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