The profile of urinary lipid metabolites in healthy dogs

Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect infla...

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Autores principales: KIDA, Taiki, YAMAZAKI, Arisa, KOBAYASHI, Koji, NAKAMURA, Tatsuro, NAKAGAWA, Takayuki, NISHIMURA, Ryohei, MURATA, Takahisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177396/
https://www.ncbi.nlm.nih.gov/pubmed/35321995
http://dx.doi.org/10.1292/jvms.22-0020
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author KIDA, Taiki
YAMAZAKI, Arisa
KOBAYASHI, Koji
NAKAMURA, Tatsuro
NAKAGAWA, Takayuki
NISHIMURA, Ryohei
MURATA, Takahisa
author_facet KIDA, Taiki
YAMAZAKI, Arisa
KOBAYASHI, Koji
NAKAMURA, Tatsuro
NAKAGAWA, Takayuki
NISHIMURA, Ryohei
MURATA, Takahisa
author_sort KIDA, Taiki
collection PubMed
description Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.
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spelling pubmed-91773962022-06-13 The profile of urinary lipid metabolites in healthy dogs KIDA, Taiki YAMAZAKI, Arisa KOBAYASHI, Koji NAKAMURA, Tatsuro NAKAGAWA, Takayuki NISHIMURA, Ryohei MURATA, Takahisa J Vet Med Sci Physiology Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases. The Japanese Society of Veterinary Science 2022-03-23 2022-05 /pmc/articles/PMC9177396/ /pubmed/35321995 http://dx.doi.org/10.1292/jvms.22-0020 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Physiology
KIDA, Taiki
YAMAZAKI, Arisa
KOBAYASHI, Koji
NAKAMURA, Tatsuro
NAKAGAWA, Takayuki
NISHIMURA, Ryohei
MURATA, Takahisa
The profile of urinary lipid metabolites in healthy dogs
title The profile of urinary lipid metabolites in healthy dogs
title_full The profile of urinary lipid metabolites in healthy dogs
title_fullStr The profile of urinary lipid metabolites in healthy dogs
title_full_unstemmed The profile of urinary lipid metabolites in healthy dogs
title_short The profile of urinary lipid metabolites in healthy dogs
title_sort profile of urinary lipid metabolites in healthy dogs
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177396/
https://www.ncbi.nlm.nih.gov/pubmed/35321995
http://dx.doi.org/10.1292/jvms.22-0020
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