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3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors

In the gut ecosystem, microorganisms regulate group behaviour and interplay with the host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human gut microbiota, exerts anti-inflammatory effects and could play a role in inflammatory bowel disease...

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Autores principales: Coquant, Garance, Aguanno, Doriane, Brot, Loïc, Belloir, Christine, Delugeard, Julie, Roger, Nathalie, Pham, Hang-Phuong, Briand, Loïc, Moreau, Marielle, de Sordi, Luisa, Carrière, Véronique, Grill, Jean-Pierre, Thenet, Sophie, Seksik, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177545/
https://www.ncbi.nlm.nih.gov/pubmed/35676403
http://dx.doi.org/10.1038/s41598-022-13451-3
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author Coquant, Garance
Aguanno, Doriane
Brot, Loïc
Belloir, Christine
Delugeard, Julie
Roger, Nathalie
Pham, Hang-Phuong
Briand, Loïc
Moreau, Marielle
de Sordi, Luisa
Carrière, Véronique
Grill, Jean-Pierre
Thenet, Sophie
Seksik, Philippe
author_facet Coquant, Garance
Aguanno, Doriane
Brot, Loïc
Belloir, Christine
Delugeard, Julie
Roger, Nathalie
Pham, Hang-Phuong
Briand, Loïc
Moreau, Marielle
de Sordi, Luisa
Carrière, Véronique
Grill, Jean-Pierre
Thenet, Sophie
Seksik, Philippe
author_sort Coquant, Garance
collection PubMed
description In the gut ecosystem, microorganisms regulate group behaviour and interplay with the host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human gut microbiota, exerts anti-inflammatory effects and could play a role in inflammatory bowel diseases where dysbiosis has been described. Our aim was to identify which signalling pathways are involved in this effect. We observed that 3-oxo-C12:2-HSL decreases expression of pro-inflammatory cytokines such as Interleukine-1β (− 35%) and Tumor Necrosis Factor-α (TNFα) (− 40%) by stimulated immune RAW264.7 cells and decreased TNF secretion by stimulated PBMC in a dose-dependent manner, between 25 to 100 µM. Transcriptomic analysis of RAW264.7 cells exposed to 3-oxo-C12:2-HSL, in a pro-inflammatory context, highlighted JAK-STAT, NF-κB and TFN signalling pathways and we confirmed that 3-oxo-C12:2-HSL inhibited JAK1 and STAT1 phosphorylation. We also showed through a screening assay that 3-oxo-C12:2-HSL interacted with several human bitter taste receptors. Its anti-inflammatory effect involved TAS2R38 as shown by pharmacologic inhibition and led to an increase in intracellular calcium levels. We thus unravelled the involvement of several cellular pathways in the anti-inflammatory effects exerted by the QS molecule 3-oxo-C12:2-HSL.
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spelling pubmed-91775452022-06-10 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors Coquant, Garance Aguanno, Doriane Brot, Loïc Belloir, Christine Delugeard, Julie Roger, Nathalie Pham, Hang-Phuong Briand, Loïc Moreau, Marielle de Sordi, Luisa Carrière, Véronique Grill, Jean-Pierre Thenet, Sophie Seksik, Philippe Sci Rep Article In the gut ecosystem, microorganisms regulate group behaviour and interplay with the host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human gut microbiota, exerts anti-inflammatory effects and could play a role in inflammatory bowel diseases where dysbiosis has been described. Our aim was to identify which signalling pathways are involved in this effect. We observed that 3-oxo-C12:2-HSL decreases expression of pro-inflammatory cytokines such as Interleukine-1β (− 35%) and Tumor Necrosis Factor-α (TNFα) (− 40%) by stimulated immune RAW264.7 cells and decreased TNF secretion by stimulated PBMC in a dose-dependent manner, between 25 to 100 µM. Transcriptomic analysis of RAW264.7 cells exposed to 3-oxo-C12:2-HSL, in a pro-inflammatory context, highlighted JAK-STAT, NF-κB and TFN signalling pathways and we confirmed that 3-oxo-C12:2-HSL inhibited JAK1 and STAT1 phosphorylation. We also showed through a screening assay that 3-oxo-C12:2-HSL interacted with several human bitter taste receptors. Its anti-inflammatory effect involved TAS2R38 as shown by pharmacologic inhibition and led to an increase in intracellular calcium levels. We thus unravelled the involvement of several cellular pathways in the anti-inflammatory effects exerted by the QS molecule 3-oxo-C12:2-HSL. Nature Publishing Group UK 2022-06-08 /pmc/articles/PMC9177545/ /pubmed/35676403 http://dx.doi.org/10.1038/s41598-022-13451-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Coquant, Garance
Aguanno, Doriane
Brot, Loïc
Belloir, Christine
Delugeard, Julie
Roger, Nathalie
Pham, Hang-Phuong
Briand, Loïc
Moreau, Marielle
de Sordi, Luisa
Carrière, Véronique
Grill, Jean-Pierre
Thenet, Sophie
Seksik, Philippe
3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title_full 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title_fullStr 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title_full_unstemmed 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title_short 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
title_sort 3-oxo-c12:2-hsl, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177545/
https://www.ncbi.nlm.nih.gov/pubmed/35676403
http://dx.doi.org/10.1038/s41598-022-13451-3
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