A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving protein production by the non-conventional yeast Komagataella phaffii comprised of genome-wide screening for effective gene disruptions, combining them...

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Detalles Bibliográficos
Autores principales: Ito, Yoichiro, Ishigami, Misa, Terai, Goro, Nakamura, Yasuyuki, Hashiba, Noriko, Nishi, Teruyuki, Nakazawa, Hikaru, Hasunuma, Tomohisa, Asai, Kiyoshi, Umetsu, Mitsuo, Ishii, Jun, Kondo, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177720/
https://www.ncbi.nlm.nih.gov/pubmed/35676418
http://dx.doi.org/10.1038/s42003-022-03475-w

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