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Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion

OBJECTIVE: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralizati...

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Autores principales: Barcellos Fernandes, Raphaella, Bárbara Polo, Ana, Novaes Rocha, Vinicius, Willer Farinazzo Vitral, Robert, Carolina Morais Apolônio, Ana, José da Silva Campos, Marcio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177871/
https://www.ncbi.nlm.nih.gov/pubmed/35692239
http://dx.doi.org/10.1016/j.sdentj.2022.03.008
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author Barcellos Fernandes, Raphaella
Bárbara Polo, Ana
Novaes Rocha, Vinicius
Willer Farinazzo Vitral, Robert
Carolina Morais Apolônio, Ana
José da Silva Campos, Marcio
author_facet Barcellos Fernandes, Raphaella
Bárbara Polo, Ana
Novaes Rocha, Vinicius
Willer Farinazzo Vitral, Robert
Carolina Morais Apolônio, Ana
José da Silva Campos, Marcio
author_sort Barcellos Fernandes, Raphaella
collection PubMed
description OBJECTIVE: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralization from a microbiological perspective. MATERIALS AND METHODS: Twenty-two brackets of each group were used. The brackets’ surface roughness was defined and the bacterial adhesion was performed using the strain S. mutans ATCC25175 with 8 h or 24 h of incubation time. The total bacterial adhesion (TBA) of biofilms was assessed using optical density (OD) methodology. To quantify bacteria viability (BV), the colony forming units (CFU) were counted. A scanning electron microscopy (SEM) observation of biofilms was also performed. Results: Ceramic brackets exhibited significantly higher roughness (0.304) compared to CM (0.090) and SLM (0.067) ones (C > CM = SLM). The data obtained with the TBA and BV tests showed that S. mutans biofilm formed on bracket groups exhibited similar results for both incubation periods. From the SEM images it is possible to observe that biofilm structure formed for 24 h was denser than that for 8 h of incubation with significantly more aggregates and cells for three groups. CONCLUSION: This in vitro study suggests that despite the higher surface roughness of ceramic brackets, this alone does not influence the adhesion of the S. mutans biofilms. CLINICAL RELEVANCE: From a microbiological perspective, the bracket's design may be more relevant than its surface roughness with respect to the adhesion of cariogenic bacteria biofilm with potential risk to dental enamel integrity.
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spelling pubmed-91778712022-06-10 Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion Barcellos Fernandes, Raphaella Bárbara Polo, Ana Novaes Rocha, Vinicius Willer Farinazzo Vitral, Robert Carolina Morais Apolônio, Ana José da Silva Campos, Marcio Saudi Dent J Original Article OBJECTIVE: To compare the surface properties of self-ligating metallic (SLM), ceramic esthetic, and conventional metallic (CM) brackets, and evaluate the adhesion of Streptococcus mutans biofilms to their surface, attempting to interpret the correlation between bracket type and enamel demineralization from a microbiological perspective. MATERIALS AND METHODS: Twenty-two brackets of each group were used. The brackets’ surface roughness was defined and the bacterial adhesion was performed using the strain S. mutans ATCC25175 with 8 h or 24 h of incubation time. The total bacterial adhesion (TBA) of biofilms was assessed using optical density (OD) methodology. To quantify bacteria viability (BV), the colony forming units (CFU) were counted. A scanning electron microscopy (SEM) observation of biofilms was also performed. Results: Ceramic brackets exhibited significantly higher roughness (0.304) compared to CM (0.090) and SLM (0.067) ones (C > CM = SLM). The data obtained with the TBA and BV tests showed that S. mutans biofilm formed on bracket groups exhibited similar results for both incubation periods. From the SEM images it is possible to observe that biofilm structure formed for 24 h was denser than that for 8 h of incubation with significantly more aggregates and cells for three groups. CONCLUSION: This in vitro study suggests that despite the higher surface roughness of ceramic brackets, this alone does not influence the adhesion of the S. mutans biofilms. CLINICAL RELEVANCE: From a microbiological perspective, the bracket's design may be more relevant than its surface roughness with respect to the adhesion of cariogenic bacteria biofilm with potential risk to dental enamel integrity. Elsevier 2022-05 2022-03-29 /pmc/articles/PMC9177871/ /pubmed/35692239 http://dx.doi.org/10.1016/j.sdentj.2022.03.008 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Barcellos Fernandes, Raphaella
Bárbara Polo, Ana
Novaes Rocha, Vinicius
Willer Farinazzo Vitral, Robert
Carolina Morais Apolônio, Ana
José da Silva Campos, Marcio
Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title_full Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title_fullStr Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title_full_unstemmed Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title_short Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion
title_sort influence of orthodontic brackets design and surface properties on the cariogenic streptococcus mutans adhesion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177871/
https://www.ncbi.nlm.nih.gov/pubmed/35692239
http://dx.doi.org/10.1016/j.sdentj.2022.03.008
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