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Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli
The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177960/ https://www.ncbi.nlm.nih.gov/pubmed/35640608 http://dx.doi.org/10.1093/nar/gkac442 |
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author | Zhang, Ya Chen, Weizhong Wu, Di Liu, Yushi Wu, Zhaowei Li, Jian Zhang, Shu-Yu Ji, Quanjiang |
author_facet | Zhang, Ya Chen, Weizhong Wu, Di Liu, Yushi Wu, Zhaowei Li, Jian Zhang, Shu-Yu Ji, Quanjiang |
author_sort | Zhang, Ya |
collection | PubMed |
description | The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli. |
format | Online Article Text |
id | pubmed-9177960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-91779602022-06-09 Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli Zhang, Ya Chen, Weizhong Wu, Di Liu, Yushi Wu, Zhaowei Li, Jian Zhang, Shu-Yu Ji, Quanjiang Nucleic Acids Res Structural Biology The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli. Oxford University Press 2022-05-30 /pmc/articles/PMC9177960/ /pubmed/35640608 http://dx.doi.org/10.1093/nar/gkac442 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Structural Biology Zhang, Ya Chen, Weizhong Wu, Di Liu, Yushi Wu, Zhaowei Li, Jian Zhang, Shu-Yu Ji, Quanjiang Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title | Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title_full | Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title_fullStr | Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title_full_unstemmed | Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title_short | Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli |
title_sort | molecular basis for cell-wall recycling regulation by transcriptional repressor murr in escherichia coli |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177960/ https://www.ncbi.nlm.nih.gov/pubmed/35640608 http://dx.doi.org/10.1093/nar/gkac442 |
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