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Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development
The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178232/ https://www.ncbi.nlm.nih.gov/pubmed/35693168 http://dx.doi.org/10.3389/fpls.2022.892077 |
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author | Yu, Hui Wang, Qiushi Zhang, Zhirui Wu, Tao Yang, Xinjing Zhu, Xiaobin Ye, Yongheng Leng, Jiantian Yang, Suxin Feng, Xianzhong |
author_facet | Yu, Hui Wang, Qiushi Zhang, Zhirui Wu, Tao Yang, Xinjing Zhu, Xiaobin Ye, Yongheng Leng, Jiantian Yang, Suxin Feng, Xianzhong |
author_sort | Yu, Hui |
collection | PubMed |
description | The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the soybean mutagenized population. The Gmpgl3-1 mutant presented with decreased chlorophyll contents, reduced chloroplast stroma thylakoids, reduced yields, and decreased numbers of pods per plant. Bulked segregant analysis (BSA) together with map-based cloning revealed a single-nucleotide non-synonymous mutation at the 341st nucleotide of the first exon of the chloroplast development-related GmTic110a gene. The phenotype of the knockout plants was the same as that of the mutant. The GmTic110a gene was highly expressed in the leaves at various developmental stages, and its protein was localized to the inner chloroplast membrane. Split luciferase complementation assays and coimmunoprecipitation (co-IP) experiments revealed that GmTic110a interacted with GmTic20, GmTic40a, and GmTic40b in tobacco leaves. These results indicated that the GmTic110a gene plays an important role in chloroplast development. |
format | Online Article Text |
id | pubmed-9178232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91782322022-06-10 Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development Yu, Hui Wang, Qiushi Zhang, Zhirui Wu, Tao Yang, Xinjing Zhu, Xiaobin Ye, Yongheng Leng, Jiantian Yang, Suxin Feng, Xianzhong Front Plant Sci Plant Science The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the soybean mutagenized population. The Gmpgl3-1 mutant presented with decreased chlorophyll contents, reduced chloroplast stroma thylakoids, reduced yields, and decreased numbers of pods per plant. Bulked segregant analysis (BSA) together with map-based cloning revealed a single-nucleotide non-synonymous mutation at the 341st nucleotide of the first exon of the chloroplast development-related GmTic110a gene. The phenotype of the knockout plants was the same as that of the mutant. The GmTic110a gene was highly expressed in the leaves at various developmental stages, and its protein was localized to the inner chloroplast membrane. Split luciferase complementation assays and coimmunoprecipitation (co-IP) experiments revealed that GmTic110a interacted with GmTic20, GmTic40a, and GmTic40b in tobacco leaves. These results indicated that the GmTic110a gene plays an important role in chloroplast development. Frontiers Media S.A. 2022-05-26 /pmc/articles/PMC9178232/ /pubmed/35693168 http://dx.doi.org/10.3389/fpls.2022.892077 Text en Copyright © 2022 Yu, Wang, Zhang, Wu, Yang, Zhu, Ye, Leng, Yang and Feng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yu, Hui Wang, Qiushi Zhang, Zhirui Wu, Tao Yang, Xinjing Zhu, Xiaobin Ye, Yongheng Leng, Jiantian Yang, Suxin Feng, Xianzhong Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title_full | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title_fullStr | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title_full_unstemmed | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title_short | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
title_sort | genetic mapping of the gmpgl3 mutant reveals the function of gmtic110a in soybean chloroplast development |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178232/ https://www.ncbi.nlm.nih.gov/pubmed/35693168 http://dx.doi.org/10.3389/fpls.2022.892077 |
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