Cargando…

Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali

BACKGROUND AND AIM: To effectively control dengue hemorrhagic fever (DHF), it is necessary to assess the risk of vertical virus transmission in Aedes aegypti mosquitoes. This study aimed to detect dengue virus (DENV) transovarial transmission in A. aegypti collected from DHF patients’ residences in...

Descripción completa

Detalles Bibliográficos
Autores principales: Sudarmaja, I. Made, Swastika, I. Kadek, Diarthini, Luh Putu Eka, Prasetya, I. Putu Dema, Wirawan, I. Md. Ady
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178568/
https://www.ncbi.nlm.nih.gov/pubmed/35698490
http://dx.doi.org/10.14202/vetworld.2022.1149-1153
_version_ 1784723087339552768
author Sudarmaja, I. Made
Swastika, I. Kadek
Diarthini, Luh Putu Eka
Prasetya, I. Putu Dema
Wirawan, I. Md. Ady
author_facet Sudarmaja, I. Made
Swastika, I. Kadek
Diarthini, Luh Putu Eka
Prasetya, I. Putu Dema
Wirawan, I. Md. Ady
author_sort Sudarmaja, I. Made
collection PubMed
description BACKGROUND AND AIM: To effectively control dengue hemorrhagic fever (DHF), it is necessary to assess the risk of vertical virus transmission in Aedes aegypti mosquitoes. This study aimed to detect dengue virus (DENV) transovarial transmission in A. aegypti collected from DHF patients’ residences in Denpasar, Bali. MATERIALS AND METHODS: A. aegypti samples were acquired by rearing A. aegypti eggs collected from ovitraps placed in the homes of DHF patients. Ovitraps were installed for 7 days and viewed using a loupe to determine whether there were Aedes spp. eggs present. An immunocytochemical method was utilized with 200 samples, and virus detection was performed using a reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Of the 10 DHF patient houses fitted with ovitraps, four produced positive ovitraps from which larvae developed (house index=40%). Of the 50 ovitraps mounted in the 10 homes, 14 ovitraps were positive and contained A. aegypti eggs (ovitrap index=28%). Of these 14 positive ovitraps containing A. aegypti eggs, 10 ovitraps produced larvae. Immunocytochemical tests were conducted on A. aegypti eggs from the four houses under study. It was found that from the 200 samples collected, 197 samples could be observed, and 11 samples (5.6%) were positive for DENV antigen. RT-PCR examination conducted on mosquitoes reared from the four houses studied obtained a negative virus content result. CONCLUSION: This study found the presence of DENV antigen to be as high as 5.6%. This means that potential for transovarial transmission exists within DHF patients’ homes in Denpasar, Bali. Aedes control strategy in Denpasar should address this finding, in addition to the current approaches which have focused primarily on the elimination of larval breeding habitats and control of adults using insecticidal fogging during outbreaks.
format Online
Article
Text
id pubmed-9178568
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-91785682022-06-12 Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali Sudarmaja, I. Made Swastika, I. Kadek Diarthini, Luh Putu Eka Prasetya, I. Putu Dema Wirawan, I. Md. Ady Vet World Research Article BACKGROUND AND AIM: To effectively control dengue hemorrhagic fever (DHF), it is necessary to assess the risk of vertical virus transmission in Aedes aegypti mosquitoes. This study aimed to detect dengue virus (DENV) transovarial transmission in A. aegypti collected from DHF patients’ residences in Denpasar, Bali. MATERIALS AND METHODS: A. aegypti samples were acquired by rearing A. aegypti eggs collected from ovitraps placed in the homes of DHF patients. Ovitraps were installed for 7 days and viewed using a loupe to determine whether there were Aedes spp. eggs present. An immunocytochemical method was utilized with 200 samples, and virus detection was performed using a reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Of the 10 DHF patient houses fitted with ovitraps, four produced positive ovitraps from which larvae developed (house index=40%). Of the 50 ovitraps mounted in the 10 homes, 14 ovitraps were positive and contained A. aegypti eggs (ovitrap index=28%). Of these 14 positive ovitraps containing A. aegypti eggs, 10 ovitraps produced larvae. Immunocytochemical tests were conducted on A. aegypti eggs from the four houses under study. It was found that from the 200 samples collected, 197 samples could be observed, and 11 samples (5.6%) were positive for DENV antigen. RT-PCR examination conducted on mosquitoes reared from the four houses studied obtained a negative virus content result. CONCLUSION: This study found the presence of DENV antigen to be as high as 5.6%. This means that potential for transovarial transmission exists within DHF patients’ homes in Denpasar, Bali. Aedes control strategy in Denpasar should address this finding, in addition to the current approaches which have focused primarily on the elimination of larval breeding habitats and control of adults using insecticidal fogging during outbreaks. Veterinary World 2022-04 2022-04-30 /pmc/articles/PMC9178568/ /pubmed/35698490 http://dx.doi.org/10.14202/vetworld.2022.1149-1153 Text en Copyright: © Sudarmaja, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sudarmaja, I. Made
Swastika, I. Kadek
Diarthini, Luh Putu Eka
Prasetya, I. Putu Dema
Wirawan, I. Md. Ady
Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title_full Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title_fullStr Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title_full_unstemmed Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title_short Dengue virus transovarial transmission detection in Aedes aegypti from dengue hemorrhagic fever patients’ residences in Denpasar, Bali
title_sort dengue virus transovarial transmission detection in aedes aegypti from dengue hemorrhagic fever patients’ residences in denpasar, bali
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178568/
https://www.ncbi.nlm.nih.gov/pubmed/35698490
http://dx.doi.org/10.14202/vetworld.2022.1149-1153
work_keys_str_mv AT sudarmajaimade denguevirustransovarialtransmissiondetectioninaedesaegyptifromdenguehemorrhagicfeverpatientsresidencesindenpasarbali
AT swastikaikadek denguevirustransovarialtransmissiondetectioninaedesaegyptifromdenguehemorrhagicfeverpatientsresidencesindenpasarbali
AT diarthiniluhputueka denguevirustransovarialtransmissiondetectioninaedesaegyptifromdenguehemorrhagicfeverpatientsresidencesindenpasarbali
AT prasetyaiputudema denguevirustransovarialtransmissiondetectioninaedesaegyptifromdenguehemorrhagicfeverpatientsresidencesindenpasarbali
AT wirawanimdady denguevirustransovarialtransmissiondetectioninaedesaegyptifromdenguehemorrhagicfeverpatientsresidencesindenpasarbali