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Transcriptional and post-transcriptional regulation of young genes in plants
BACKGROUND: New genes continuously emerge from non-coding DNA or by diverging from existing genes, but most of them are rapidly lost and only a few become fixed within the population. We hypothesized that young genes are subject to transcriptional and post-transcriptional regulation to limit their e...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178820/ https://www.ncbi.nlm.nih.gov/pubmed/35676681 http://dx.doi.org/10.1186/s12915-022-01339-7 |
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author | Raxwal, Vivek Kumar Singh, Somya Agarwal, Manu Riha, Karel |
author_facet | Raxwal, Vivek Kumar Singh, Somya Agarwal, Manu Riha, Karel |
author_sort | Raxwal, Vivek Kumar |
collection | PubMed |
description | BACKGROUND: New genes continuously emerge from non-coding DNA or by diverging from existing genes, but most of them are rapidly lost and only a few become fixed within the population. We hypothesized that young genes are subject to transcriptional and post-transcriptional regulation to limit their expression and minimize their exposure to purifying selection. RESULTS: We performed a protein-based homology search across the tree of life to determine the evolutionary age of protein-coding genes present in the rice genome. We found that young genes in rice have relatively low expression levels, which can be attributed to distal enhancers, and closed chromatin conformation at their transcription start sites (TSS). The chromatin in TSS regions can be re-modeled in response to abiotic stress, indicating conditional expression of young genes. Furthermore, transcripts of young genes in Arabidopsis tend to be targeted by nonsense-mediated RNA decay, presenting another layer of regulation limiting their expression. CONCLUSIONS: These data suggest that transcriptional and post-transcriptional mechanisms contribute to the conditional expression of young genes, which may alleviate purging selection while providing an opportunity for phenotypic exposure and functionalization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01339-7. |
format | Online Article Text |
id | pubmed-9178820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91788202022-06-10 Transcriptional and post-transcriptional regulation of young genes in plants Raxwal, Vivek Kumar Singh, Somya Agarwal, Manu Riha, Karel BMC Biol Research Article BACKGROUND: New genes continuously emerge from non-coding DNA or by diverging from existing genes, but most of them are rapidly lost and only a few become fixed within the population. We hypothesized that young genes are subject to transcriptional and post-transcriptional regulation to limit their expression and minimize their exposure to purifying selection. RESULTS: We performed a protein-based homology search across the tree of life to determine the evolutionary age of protein-coding genes present in the rice genome. We found that young genes in rice have relatively low expression levels, which can be attributed to distal enhancers, and closed chromatin conformation at their transcription start sites (TSS). The chromatin in TSS regions can be re-modeled in response to abiotic stress, indicating conditional expression of young genes. Furthermore, transcripts of young genes in Arabidopsis tend to be targeted by nonsense-mediated RNA decay, presenting another layer of regulation limiting their expression. CONCLUSIONS: These data suggest that transcriptional and post-transcriptional mechanisms contribute to the conditional expression of young genes, which may alleviate purging selection while providing an opportunity for phenotypic exposure and functionalization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01339-7. BioMed Central 2022-06-09 /pmc/articles/PMC9178820/ /pubmed/35676681 http://dx.doi.org/10.1186/s12915-022-01339-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Raxwal, Vivek Kumar Singh, Somya Agarwal, Manu Riha, Karel Transcriptional and post-transcriptional regulation of young genes in plants |
title | Transcriptional and post-transcriptional regulation of young genes in plants |
title_full | Transcriptional and post-transcriptional regulation of young genes in plants |
title_fullStr | Transcriptional and post-transcriptional regulation of young genes in plants |
title_full_unstemmed | Transcriptional and post-transcriptional regulation of young genes in plants |
title_short | Transcriptional and post-transcriptional regulation of young genes in plants |
title_sort | transcriptional and post-transcriptional regulation of young genes in plants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178820/ https://www.ncbi.nlm.nih.gov/pubmed/35676681 http://dx.doi.org/10.1186/s12915-022-01339-7 |
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