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Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses

BACKGROUND: Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular mat...

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Autores principales: Yamada, Ana Lucia Miluzzi, do Prado Vendruscolo, Cynthia, Marsiglia, Marília Ferrari, Sotelo, Eric Danilo Pauls, Agreste, Fernanda Rodrigues, Seidel, Sarah Raphaela Torquato, Fülber, Joice, Baccarin, Raquel Yvonne Arantes, da Silva, Luis Claudio Lopes Correia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178899/
https://www.ncbi.nlm.nih.gov/pubmed/35681208
http://dx.doi.org/10.1186/s12917-022-03323-3
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author Yamada, Ana Lucia Miluzzi
do Prado Vendruscolo, Cynthia
Marsiglia, Marília Ferrari
Sotelo, Eric Danilo Pauls
Agreste, Fernanda Rodrigues
Seidel, Sarah Raphaela Torquato
Fülber, Joice
Baccarin, Raquel Yvonne Arantes
da Silva, Luis Claudio Lopes Correia
author_facet Yamada, Ana Lucia Miluzzi
do Prado Vendruscolo, Cynthia
Marsiglia, Marília Ferrari
Sotelo, Eric Danilo Pauls
Agreste, Fernanda Rodrigues
Seidel, Sarah Raphaela Torquato
Fülber, Joice
Baccarin, Raquel Yvonne Arantes
da Silva, Luis Claudio Lopes Correia
author_sort Yamada, Ana Lucia Miluzzi
collection PubMed
description BACKGROUND: Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular matrix degradation. Although several studies have been published on this topic, the effectiveness of treatment with oral CS and GlcN remains uncertain. The objective of this study was to analyze the progression of experimentally induced osteoarthritis in horses and verify the effectiveness of an oral compound based on CS and GlcN to treat and/or modulate this disease. The study analyzed the metacarpophalangeal joint of the left thoracic limb of 16 horses divided into two groups, with eight horses treated with CS and GlcN in the treated group (GT) and eight untreated horses in the control group (GC). Chondral lesions were induced through arthroscopy, which was defined as time-point zero (T0). Physical, ultrasonographic, and radiographic examinations and synovial fluid biomarkers measurements were performed on days 0, 30, 60, 90, and 120. At the end of the experiment (T4), arthroscopy was performed again to macroscopically evaluate the joints and collect material for microscopic analysis. RESULTS: Significant differences were observed between groups in some evaluated parameters, such as visual lameness assessment, synovial concentrations of prostaglandin E2, and ultrasound examination. However, the GT still presented slightly improved results for joint flexion angle, analysis of lameness using sensors, and histopathological analysis of chondral repair tissue, however, without the statistical significance (p>0.05). CONCLUSIONS: The treatment was considered effective in the clinical modulation of experimental osteoarthritis, with improvement of some parameters in the GT. However, this type of treatment may not be entirely effective to change the catabolic process in articular cartilage and the progressive induced chondral damage.
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spelling pubmed-91788992022-06-10 Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses Yamada, Ana Lucia Miluzzi do Prado Vendruscolo, Cynthia Marsiglia, Marília Ferrari Sotelo, Eric Danilo Pauls Agreste, Fernanda Rodrigues Seidel, Sarah Raphaela Torquato Fülber, Joice Baccarin, Raquel Yvonne Arantes da Silva, Luis Claudio Lopes Correia BMC Vet Res Original Research BACKGROUND: Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular matrix degradation. Although several studies have been published on this topic, the effectiveness of treatment with oral CS and GlcN remains uncertain. The objective of this study was to analyze the progression of experimentally induced osteoarthritis in horses and verify the effectiveness of an oral compound based on CS and GlcN to treat and/or modulate this disease. The study analyzed the metacarpophalangeal joint of the left thoracic limb of 16 horses divided into two groups, with eight horses treated with CS and GlcN in the treated group (GT) and eight untreated horses in the control group (GC). Chondral lesions were induced through arthroscopy, which was defined as time-point zero (T0). Physical, ultrasonographic, and radiographic examinations and synovial fluid biomarkers measurements were performed on days 0, 30, 60, 90, and 120. At the end of the experiment (T4), arthroscopy was performed again to macroscopically evaluate the joints and collect material for microscopic analysis. RESULTS: Significant differences were observed between groups in some evaluated parameters, such as visual lameness assessment, synovial concentrations of prostaglandin E2, and ultrasound examination. However, the GT still presented slightly improved results for joint flexion angle, analysis of lameness using sensors, and histopathological analysis of chondral repair tissue, however, without the statistical significance (p>0.05). CONCLUSIONS: The treatment was considered effective in the clinical modulation of experimental osteoarthritis, with improvement of some parameters in the GT. However, this type of treatment may not be entirely effective to change the catabolic process in articular cartilage and the progressive induced chondral damage. BioMed Central 2022-06-09 /pmc/articles/PMC9178899/ /pubmed/35681208 http://dx.doi.org/10.1186/s12917-022-03323-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Original Research
Yamada, Ana Lucia Miluzzi
do Prado Vendruscolo, Cynthia
Marsiglia, Marília Ferrari
Sotelo, Eric Danilo Pauls
Agreste, Fernanda Rodrigues
Seidel, Sarah Raphaela Torquato
Fülber, Joice
Baccarin, Raquel Yvonne Arantes
da Silva, Luis Claudio Lopes Correia
Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title_full Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title_fullStr Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title_full_unstemmed Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title_short Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
title_sort effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178899/
https://www.ncbi.nlm.nih.gov/pubmed/35681208
http://dx.doi.org/10.1186/s12917-022-03323-3
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