Cargando…

The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats

Radiation-induced lung injuries (RILI) is one of the serious complications of radiotherapy posed by the damage of alveolar cells and inflammation over-reaction. We aimed to investigate the potential protective effects of doxepin on RILI (20 Gy total dose at 3 Gy/min of X-ray irradiation), as well as...

Descripción completa

Detalles Bibliográficos
Autores principales: Wan, Xinlong, Shi, Xuan, Li, Mengke, Chen, Qing, Xue, Chang, Li, Guanghui, Huang, Yeke, Yang, Jingwen, Chen, Chan, Wang, Zhiyi, Ma, Shumei, Liu, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178985/
https://www.ncbi.nlm.nih.gov/pubmed/35693872
http://dx.doi.org/10.1177/15593258221107193
_version_ 1784723173676154880
author Wan, Xinlong
Shi, Xuan
Li, Mengke
Chen, Qing
Xue, Chang
Li, Guanghui
Huang, Yeke
Yang, Jingwen
Chen, Chan
Wang, Zhiyi
Ma, Shumei
Liu, Xiaodong
author_facet Wan, Xinlong
Shi, Xuan
Li, Mengke
Chen, Qing
Xue, Chang
Li, Guanghui
Huang, Yeke
Yang, Jingwen
Chen, Chan
Wang, Zhiyi
Ma, Shumei
Liu, Xiaodong
author_sort Wan, Xinlong
collection PubMed
description Radiation-induced lung injuries (RILI) is one of the serious complications of radiotherapy posed by the damage of alveolar cells and inflammation over-reaction. We aimed to investigate the potential protective effects of doxepin on RILI (20 Gy total dose at 3 Gy/min of X-ray irradiation), as well as its underlying mechanism. For animal experiments, such parameters as Immunohistochemistry and hematoxylin and eosin (H&E) staining, WBC (white blood cell), CRP (C-reactive protein), Western blot, and q-PCR were detected. The results indicated that both survival status and weight increase of irradiated rats treated by doxepin (3 mg/kg/day, rat) were higher than those of treated with irradiation alone (Dosing started the day before irradiation). Further, histological examinations showed doxepin could tenuate the radiation injury, as indicated as alveolar inflammatory exudation and there was only mild interstitial inflammation infiltration. Western blotting and q-PCR showed that expression of NF-κβ in X group were higher than that in XMD group. For the first time, we reported doxepin functioned as a radioprotectant candidate, which provide a promising application of doxepin for protecting radiotherapy injuries.
format Online
Article
Text
id pubmed-9178985
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-91789852022-06-10 The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats Wan, Xinlong Shi, Xuan Li, Mengke Chen, Qing Xue, Chang Li, Guanghui Huang, Yeke Yang, Jingwen Chen, Chan Wang, Zhiyi Ma, Shumei Liu, Xiaodong Dose Response Original Article Radiation-induced lung injuries (RILI) is one of the serious complications of radiotherapy posed by the damage of alveolar cells and inflammation over-reaction. We aimed to investigate the potential protective effects of doxepin on RILI (20 Gy total dose at 3 Gy/min of X-ray irradiation), as well as its underlying mechanism. For animal experiments, such parameters as Immunohistochemistry and hematoxylin and eosin (H&E) staining, WBC (white blood cell), CRP (C-reactive protein), Western blot, and q-PCR were detected. The results indicated that both survival status and weight increase of irradiated rats treated by doxepin (3 mg/kg/day, rat) were higher than those of treated with irradiation alone (Dosing started the day before irradiation). Further, histological examinations showed doxepin could tenuate the radiation injury, as indicated as alveolar inflammatory exudation and there was only mild interstitial inflammation infiltration. Western blotting and q-PCR showed that expression of NF-κβ in X group were higher than that in XMD group. For the first time, we reported doxepin functioned as a radioprotectant candidate, which provide a promising application of doxepin for protecting radiotherapy injuries. SAGE Publications 2022-06-07 /pmc/articles/PMC9178985/ /pubmed/35693872 http://dx.doi.org/10.1177/15593258221107193 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Wan, Xinlong
Shi, Xuan
Li, Mengke
Chen, Qing
Xue, Chang
Li, Guanghui
Huang, Yeke
Yang, Jingwen
Chen, Chan
Wang, Zhiyi
Ma, Shumei
Liu, Xiaodong
The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title_full The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title_fullStr The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title_full_unstemmed The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title_short The Protective Effects and Mechanism of Doxepin on Radiation–Induced Lung Injury in Rats
title_sort protective effects and mechanism of doxepin on radiation–induced lung injury in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178985/
https://www.ncbi.nlm.nih.gov/pubmed/35693872
http://dx.doi.org/10.1177/15593258221107193
work_keys_str_mv AT wanxinlong theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT shixuan theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT limengke theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT chenqing theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT xuechang theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT liguanghui theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT huangyeke theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT yangjingwen theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT chenchan theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT wangzhiyi theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT mashumei theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT liuxiaodong theprotectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT wanxinlong protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT shixuan protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT limengke protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT chenqing protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT xuechang protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT liguanghui protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT huangyeke protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT yangjingwen protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT chenchan protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT wangzhiyi protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT mashumei protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats
AT liuxiaodong protectiveeffectsandmechanismofdoxepinonradiationinducedlunginjuryinrats