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Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria
The effects of lipid peroxidation products 4‐Hydroxy‐2‐nonenal (4‐HNE) and 4‐oxo‐2‐nonenal (4‐ONE) were evaluated using bovine heart mitochondria. Oxygen consumption rate (OCR), ultrastructure, antioxidant activity, and membrane permeability were examined to compare their effects on isolated mitocho...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179123/ https://www.ncbi.nlm.nih.gov/pubmed/35702292 http://dx.doi.org/10.1002/fsn3.2799 |
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author | Mohan, Anand Kafle, Deepak Singh, Rakesh K. Hung, Yen‐Con |
author_facet | Mohan, Anand Kafle, Deepak Singh, Rakesh K. Hung, Yen‐Con |
author_sort | Mohan, Anand |
collection | PubMed |
description | The effects of lipid peroxidation products 4‐Hydroxy‐2‐nonenal (4‐HNE) and 4‐oxo‐2‐nonenal (4‐ONE) were evaluated using bovine heart mitochondria. Oxygen consumption rate (OCR), ultrastructure, antioxidant activity, and membrane permeability were examined to compare their effects on isolated mitochondria from beef cardiac muscle. For the mitochondrial morphology, the final concentration of mitochondria and 4‐ONE or 4‐HNE in the reaction tube were 10 mg/ml and 1 mM, respectively. For the OCR experiment, mitochondria (2.5 mg/ml) were incubated with 0.20 mM ONE or in a Clark electrode chamber at 25°C. Mitochondrial membrane permeability was determined by incubating 0.5 mg/ml of mitochondrial protein with either 0.05 mM ONE or HNE or ethanol control at pH 5.6 and 7.4 at 25°C. Transmission electron microscopy (TEM) revealed that the size of 4‐ONE treated mitochondria at pH 7.4 increased (p < .05), as did permeability (p < .05), unlike ethanol controls. However, mitochondria incubated with 4‐ONE at pH 5.6 showed a decrease in volume (p < .05). Incubating mitochondria with 4‐ONE at pH 5.6 and pH increased oxygen consumption rate 7.4 caused less oxygen consumption than either 4‐HNE treatment or ethanol control. The hydrogen peroxide assay (H(2)O(2)), ferric reducing antioxidant properties (FRAP), and 2,2’‐azinobis (3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS.(+)) assays revealed that 4‐ONE is a more potent inhibitor of the endogenous antioxidant system of mitochondria than 4‐HNE (p < .05). |
format | Online Article Text |
id | pubmed-9179123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91791232022-06-13 Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria Mohan, Anand Kafle, Deepak Singh, Rakesh K. Hung, Yen‐Con Food Sci Nutr Original Articles The effects of lipid peroxidation products 4‐Hydroxy‐2‐nonenal (4‐HNE) and 4‐oxo‐2‐nonenal (4‐ONE) were evaluated using bovine heart mitochondria. Oxygen consumption rate (OCR), ultrastructure, antioxidant activity, and membrane permeability were examined to compare their effects on isolated mitochondria from beef cardiac muscle. For the mitochondrial morphology, the final concentration of mitochondria and 4‐ONE or 4‐HNE in the reaction tube were 10 mg/ml and 1 mM, respectively. For the OCR experiment, mitochondria (2.5 mg/ml) were incubated with 0.20 mM ONE or in a Clark electrode chamber at 25°C. Mitochondrial membrane permeability was determined by incubating 0.5 mg/ml of mitochondrial protein with either 0.05 mM ONE or HNE or ethanol control at pH 5.6 and 7.4 at 25°C. Transmission electron microscopy (TEM) revealed that the size of 4‐ONE treated mitochondria at pH 7.4 increased (p < .05), as did permeability (p < .05), unlike ethanol controls. However, mitochondria incubated with 4‐ONE at pH 5.6 showed a decrease in volume (p < .05). Incubating mitochondria with 4‐ONE at pH 5.6 and pH increased oxygen consumption rate 7.4 caused less oxygen consumption than either 4‐HNE treatment or ethanol control. The hydrogen peroxide assay (H(2)O(2)), ferric reducing antioxidant properties (FRAP), and 2,2’‐azinobis (3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS.(+)) assays revealed that 4‐ONE is a more potent inhibitor of the endogenous antioxidant system of mitochondria than 4‐HNE (p < .05). John Wiley and Sons Inc. 2022-03-09 /pmc/articles/PMC9179123/ /pubmed/35702292 http://dx.doi.org/10.1002/fsn3.2799 Text en © 2022 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Mohan, Anand Kafle, Deepak Singh, Rakesh K. Hung, Yen‐Con Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title | Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title_full | Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title_fullStr | Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title_full_unstemmed | Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title_short | Effects of 4‐Oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
title_sort | effects of 4‐oxo‐2‐nonenal on biochemical properties of bovine heart mitochondria |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179123/ https://www.ncbi.nlm.nih.gov/pubmed/35702292 http://dx.doi.org/10.1002/fsn3.2799 |
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