Cargando…

Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis

SIMPLE SUMMARY: Collecting specimens for pathological diagnosis of malignant biliary stricture (MBS) is not easy. This study shows that cell block (CB) of overnight-stored bile is useful for pathological diagnosis of MBS. Cancer detectability using the CB method (62.2%) is superior to that using cyt...

Descripción completa

Detalles Bibliográficos
Autores principales: Okuno, Mitsuru, Mukai, Tsuyoshi, Iwata, Keisuke, Watanabe, Naoki, Tanaka, Takuji, Iwasa, Taisei, Shimojo, Kota, Ohashi, Yosuke, Takagi, Akihiro, Ito, Yuki, Tezuka, Ryuichi, Iwata, Shota, Iwasa, Yuhei, Kochi, Takahiro, Ogiso, Tomio, Hayashi, Hideki, Sugiyama, Akihiko, Nishigaki, Youichi, Tomita, Eiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179241/
https://www.ncbi.nlm.nih.gov/pubmed/35681681
http://dx.doi.org/10.3390/cancers14112701
_version_ 1784723225702301696
author Okuno, Mitsuru
Mukai, Tsuyoshi
Iwata, Keisuke
Watanabe, Naoki
Tanaka, Takuji
Iwasa, Taisei
Shimojo, Kota
Ohashi, Yosuke
Takagi, Akihiro
Ito, Yuki
Tezuka, Ryuichi
Iwata, Shota
Iwasa, Yuhei
Kochi, Takahiro
Ogiso, Tomio
Hayashi, Hideki
Sugiyama, Akihiko
Nishigaki, Youichi
Tomita, Eiichi
author_facet Okuno, Mitsuru
Mukai, Tsuyoshi
Iwata, Keisuke
Watanabe, Naoki
Tanaka, Takuji
Iwasa, Taisei
Shimojo, Kota
Ohashi, Yosuke
Takagi, Akihiro
Ito, Yuki
Tezuka, Ryuichi
Iwata, Shota
Iwasa, Yuhei
Kochi, Takahiro
Ogiso, Tomio
Hayashi, Hideki
Sugiyama, Akihiko
Nishigaki, Youichi
Tomita, Eiichi
author_sort Okuno, Mitsuru
collection PubMed
description SIMPLE SUMMARY: Collecting specimens for pathological diagnosis of malignant biliary stricture (MBS) is not easy. This study shows that cell block (CB) of overnight-stored bile is useful for pathological diagnosis of MBS. Cancer detectability using the CB method (62.2%) is superior to that using cytology (37.8%). When the CB method is combined with biopsy, the cancer detectability (75.6%) and accuracy rates (81.4%) are increased. In addition, immunohistochemistry can be applied to the CB method when encountering difficult cases for pathological diagnosis. The CB method utilizing overnight-stored bile can be routinely used for detecting MBS in local hospitals. ABSTRACT: The specimen collection and subsequent pathological diagnosis of malignant biliary stricture (MBS) are difficult. This study aimed to determine whether the cell block (CB) method using overnight-stored bile is useful in the diagnosis of MBS. This trial was a single-arm prospective study involving a total of 59 patients with suspected MBS. The primary endpoint was cancer detectability and accuracy using the CB method, and a comparison with the detectability and accuracy achieved with bile cytology was made. The immunohistochemical sensitivity for maspin and p53 was also investigated in the CB and surgical specimens. We were able to collect bile from all 59 patients, and 45 of these patients were clinically diagnosed with MBS. The cancer detectability using the CB method (62.2%) was significantly higher than that using cytology (37.8%) (p = 0.0344). When CB was combined with biopsy, the rates of cancer detectability (75.6%) and accuracy (81.4%) increased. In eight patients who received surgical therapy, maspin- and p53-immunohistochemistry was applied to the surgical and CB specimens, and cancer cells in both specimens showed positive cytoplasmic and nuclear staining for maspin and nuclear staining for p53. The CB method is, thus, useful for detecting malignancy (UMIN000034707).
format Online
Article
Text
id pubmed-9179241
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91792412022-06-10 Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis Okuno, Mitsuru Mukai, Tsuyoshi Iwata, Keisuke Watanabe, Naoki Tanaka, Takuji Iwasa, Taisei Shimojo, Kota Ohashi, Yosuke Takagi, Akihiro Ito, Yuki Tezuka, Ryuichi Iwata, Shota Iwasa, Yuhei Kochi, Takahiro Ogiso, Tomio Hayashi, Hideki Sugiyama, Akihiko Nishigaki, Youichi Tomita, Eiichi Cancers (Basel) Article SIMPLE SUMMARY: Collecting specimens for pathological diagnosis of malignant biliary stricture (MBS) is not easy. This study shows that cell block (CB) of overnight-stored bile is useful for pathological diagnosis of MBS. Cancer detectability using the CB method (62.2%) is superior to that using cytology (37.8%). When the CB method is combined with biopsy, the cancer detectability (75.6%) and accuracy rates (81.4%) are increased. In addition, immunohistochemistry can be applied to the CB method when encountering difficult cases for pathological diagnosis. The CB method utilizing overnight-stored bile can be routinely used for detecting MBS in local hospitals. ABSTRACT: The specimen collection and subsequent pathological diagnosis of malignant biliary stricture (MBS) are difficult. This study aimed to determine whether the cell block (CB) method using overnight-stored bile is useful in the diagnosis of MBS. This trial was a single-arm prospective study involving a total of 59 patients with suspected MBS. The primary endpoint was cancer detectability and accuracy using the CB method, and a comparison with the detectability and accuracy achieved with bile cytology was made. The immunohistochemical sensitivity for maspin and p53 was also investigated in the CB and surgical specimens. We were able to collect bile from all 59 patients, and 45 of these patients were clinically diagnosed with MBS. The cancer detectability using the CB method (62.2%) was significantly higher than that using cytology (37.8%) (p = 0.0344). When CB was combined with biopsy, the rates of cancer detectability (75.6%) and accuracy (81.4%) increased. In eight patients who received surgical therapy, maspin- and p53-immunohistochemistry was applied to the surgical and CB specimens, and cancer cells in both specimens showed positive cytoplasmic and nuclear staining for maspin and nuclear staining for p53. The CB method is, thus, useful for detecting malignancy (UMIN000034707). MDPI 2022-05-30 /pmc/articles/PMC9179241/ /pubmed/35681681 http://dx.doi.org/10.3390/cancers14112701 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okuno, Mitsuru
Mukai, Tsuyoshi
Iwata, Keisuke
Watanabe, Naoki
Tanaka, Takuji
Iwasa, Taisei
Shimojo, Kota
Ohashi, Yosuke
Takagi, Akihiro
Ito, Yuki
Tezuka, Ryuichi
Iwata, Shota
Iwasa, Yuhei
Kochi, Takahiro
Ogiso, Tomio
Hayashi, Hideki
Sugiyama, Akihiko
Nishigaki, Youichi
Tomita, Eiichi
Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title_full Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title_fullStr Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title_full_unstemmed Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title_short Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis
title_sort evaluation of the cell block method using overnight-stored bile for malignant biliary stricture diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179241/
https://www.ncbi.nlm.nih.gov/pubmed/35681681
http://dx.doi.org/10.3390/cancers14112701
work_keys_str_mv AT okunomitsuru evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT mukaitsuyoshi evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT iwatakeisuke evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT watanabenaoki evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT tanakatakuji evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT iwasataisei evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT shimojokota evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT ohashiyosuke evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT takagiakihiro evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT itoyuki evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT tezukaryuichi evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT iwatashota evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT iwasayuhei evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT kochitakahiro evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT ogisotomio evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT hayashihideki evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT sugiyamaakihiko evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT nishigakiyouichi evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis
AT tomitaeiichi evaluationofthecellblockmethodusingovernightstoredbileformalignantbiliarystricturediagnosis