Label-Free Mass Spectrometry-Based Proteomic Analysis in Lamb Tissues after Fish Oil, Carnosic Acid, and Inorganic Selenium Supplementation

SIMPLE SUMMARY: Advances in proteomics and bioinformatics analysis offer the potential to investigate nutrients’ influence on protein expression profiles, and consequently on biological processes, molecular functions, and cellular components. However, knowledge in this area, particular about the exact way selenium modulates protein expression, remains limited. Therefore, in this project, global differential proteomic experiments were carried out in order to identify changes in the expression of proteins in animal tissues obtained from lambs on a specific diet involving the addition of a combination of different supplements, namely, inorganic selenium compounds, fish oil, and carnosic acid. Following inorganic selenium supplementation, a protein-protein interaction network analysis of forty differentially-expressed proteins indicated two significant clusters. ABSTRACT: Selenium is an essential nutrient, building twenty five identified selenoproteins in humans known to perform several important biological functions. The small amount of selenium in the earth’s crust in certain regions along with the risk of deficiency in organisms have resulted in increasingly popular dietary supplementation in animals, implemented via, e.g., inorganic selenium compounds. Even though selenium is included in selenoproteins in the form of selenocysteine, the dietary effect of selenium may result in the expression of other proteins or genes. Very little is known about the expression effects modulated by selenium. The present study aimed to examine the significance of protein expression in lamb tissues obtained after dietary supplementation with selenium (sodium selenate) and two other feed additives, fish oil and carnosic acid. Label-free mass spectrometry-based proteomic analysis was successfully applied to examine the animal tissues. Protein-protein interaction network analysis of forty differently-expressed proteins following inorganic selenium supplementation indicated two significant clusters which are involved in cell adhesion, heart development, actin filament-based movement, plasma membrane repair, and establishment of organelle localization.