Pathological and Tissue-Based Molecular Investigation of Granulomas in Cichlids Reared as Ornamental Fish

SIMPLE SUMMARY: The global ornamental fish trade has an estimated value of USD 15–30 billion per year and more than a 10% average annual growth. Despite their economic importance, the management of ornamental fish is challenged by a paucity of information, including data on the fish health status. Pathological and microbiological investigations were conducted on ornamental cichlids sampled during routine management activities held at an aquarium commercial facility, in order to evaluate the presence of granuloma in the organs. Cutibacterium acnes and Mycobacterium spp. were detected by molecular methods and immunohistochemistry. These bacteria represent potential zoonotic agents, and the advancement of their knowledge could significantly improve the management of ornamental fish and reduce the risk of exposure for people, such as hobbyists, fish handlers, aquarists, and dedicated personnel. ABSTRACT: Cichlids include hundreds of species with a high economic value for aquaculture. These fish are subjected to intensive trade and farming that expose them to the risk of infectious diseases. This work focuses on ornamental cichlids held in an aquarium commercial facility presenting emaciation, in order to evaluate the presence of lesions in fish skin and organs. The fish were sampled during routine management activities and subjected to pathological and molecular investigations. The presence of lymphocystis disease virus, typically associated with cutaneous nodular disease, was ruled out. Histologically, they presented granulomas in the spleen, sometimes extending to the other visceral organs. Bacterial heat-shock protein 65 PCR products were detected in tissues associated, in the majority of cases, with granulomas; molecular investigation identified Mycobacterium spp. in two cases and Cutibacterium acnes in seven cases. Immunoreactivity to anti-Mycobacterium and anti-C. acnes antibodies was detected within granulomas. The presence of C. acnes within granuloma is elucidated for the first time in fish; however, similarly to what is found in humans, this bacterium could be harmless in normal conditions, whereas other contributing factors would be required to trigger a granulomatogenous response. Further confirmation by bacterial culture, as well as using large-scale studies in more controlled situations, is needed.