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CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury

Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditio...

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Autores principales: Pannunzio, Bruno, Amo-Aparicio, Jesús, Julián, Camila, López-Vales, Rubèn, Peluffo, Hugo, Lago, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179995/
https://www.ncbi.nlm.nih.gov/pubmed/35681481
http://dx.doi.org/10.3390/cells11111786
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author Pannunzio, Bruno
Amo-Aparicio, Jesús
Julián, Camila
López-Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
author_facet Pannunzio, Bruno
Amo-Aparicio, Jesús
Julián, Camila
López-Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
author_sort Pannunzio, Bruno
collection PubMed
description Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.
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spelling pubmed-91799952022-06-10 CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury Pannunzio, Bruno Amo-Aparicio, Jesús Julián, Camila López-Vales, Rubèn Peluffo, Hugo Lago, Natalia Cells Article Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery. MDPI 2022-05-30 /pmc/articles/PMC9179995/ /pubmed/35681481 http://dx.doi.org/10.3390/cells11111786 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pannunzio, Bruno
Amo-Aparicio, Jesús
Julián, Camila
López-Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_full CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_fullStr CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_full_unstemmed CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_short CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_sort cd200r1 contributes to successful functional reinnervation after a sciatic nerve injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179995/
https://www.ncbi.nlm.nih.gov/pubmed/35681481
http://dx.doi.org/10.3390/cells11111786
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