Cargando…
Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial
Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) he...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180176/ https://www.ncbi.nlm.nih.gov/pubmed/35681488 http://dx.doi.org/10.3390/cells11111794 |
_version_ | 1784723452947595264 |
---|---|
author | González-Ravina, Cristina Santamaría-López, Esther Pacheco, Alberto Ramos, Julia Carranza, Francisco Murria, Lucía Ortiz-Vallecillo, Ana Fernández-Sánchez, Manuel |
author_facet | González-Ravina, Cristina Santamaría-López, Esther Pacheco, Alberto Ramos, Julia Carranza, Francisco Murria, Lucía Ortiz-Vallecillo, Ana Fernández-Sánchez, Manuel |
author_sort | González-Ravina, Cristina |
collection | PubMed |
description | Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics. |
format | Online Article Text |
id | pubmed-9180176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91801762022-06-10 Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial González-Ravina, Cristina Santamaría-López, Esther Pacheco, Alberto Ramos, Julia Carranza, Francisco Murria, Lucía Ortiz-Vallecillo, Ana Fernández-Sánchez, Manuel Cells Article Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics. MDPI 2022-05-30 /pmc/articles/PMC9180176/ /pubmed/35681488 http://dx.doi.org/10.3390/cells11111794 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article González-Ravina, Cristina Santamaría-López, Esther Pacheco, Alberto Ramos, Julia Carranza, Francisco Murria, Lucía Ortiz-Vallecillo, Ana Fernández-Sánchez, Manuel Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title | Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title_full | Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title_fullStr | Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title_full_unstemmed | Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title_short | Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial |
title_sort | effect of sperm selection by magnetic-activated cell sorting in d-iui: a randomized control trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180176/ https://www.ncbi.nlm.nih.gov/pubmed/35681488 http://dx.doi.org/10.3390/cells11111794 |
work_keys_str_mv | AT gonzalezravinacristina effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT santamarialopezesther effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT pachecoalberto effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT ramosjulia effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT carranzafrancisco effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT murrialucia effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT ortizvallecilloana effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial AT fernandezsanchezmanuel effectofspermselectionbymagneticactivatedcellsortingindiuiarandomizedcontroltrial |