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Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani
The flour beetle Tribolium freemani is a sibling species of the model organism and important pest Tribolium castaneum. The two species are so closely related that they can produce hybrid progeny, but the genetic basis of their differences has not been revealed. In this work, we sequenced the T. free...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180572/ https://www.ncbi.nlm.nih.gov/pubmed/35682551 http://dx.doi.org/10.3390/ijms23115869 |
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author | Volarić, Marin Despot-Slade, Evelin Veseljak, Damira Meštrović, Nevenka Mravinac, Brankica |
author_facet | Volarić, Marin Despot-Slade, Evelin Veseljak, Damira Meštrović, Nevenka Mravinac, Brankica |
author_sort | Volarić, Marin |
collection | PubMed |
description | The flour beetle Tribolium freemani is a sibling species of the model organism and important pest Tribolium castaneum. The two species are so closely related that they can produce hybrid progeny, but the genetic basis of their differences has not been revealed. In this work, we sequenced the T. freemani genome by applying PacBio HiFi technology. Using the well-assembled T. castaneum genome as a reference, we assembled 262 Mb of the T. freemani genomic sequence and anchored it in 10 linkage groups corresponding to nine autosomes and sex chromosome X. The assembly showed 99.8% completeness of conserved insect genes, indicating a high-quality reference genome. Comparison with the T. castaneum assembly revealed that the main differences in genomic sequence between the two sibling species come from repetitive DNA, including interspersed and tandem repeats. In this work, we also provided the complete assembled mitochondrial genome of T. freemani. Although the genome assembly needs to be ameliorated in tandemly repeated regions, the first version of the T. freemani reference genome and the complete mitogenome presented here represent useful resources for comparative evolutionary studies of related species and for further basic and applied research on different biological aspects of economically important pests. |
format | Online Article Text |
id | pubmed-9180572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91805722022-06-10 Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani Volarić, Marin Despot-Slade, Evelin Veseljak, Damira Meštrović, Nevenka Mravinac, Brankica Int J Mol Sci Article The flour beetle Tribolium freemani is a sibling species of the model organism and important pest Tribolium castaneum. The two species are so closely related that they can produce hybrid progeny, but the genetic basis of their differences has not been revealed. In this work, we sequenced the T. freemani genome by applying PacBio HiFi technology. Using the well-assembled T. castaneum genome as a reference, we assembled 262 Mb of the T. freemani genomic sequence and anchored it in 10 linkage groups corresponding to nine autosomes and sex chromosome X. The assembly showed 99.8% completeness of conserved insect genes, indicating a high-quality reference genome. Comparison with the T. castaneum assembly revealed that the main differences in genomic sequence between the two sibling species come from repetitive DNA, including interspersed and tandem repeats. In this work, we also provided the complete assembled mitochondrial genome of T. freemani. Although the genome assembly needs to be ameliorated in tandemly repeated regions, the first version of the T. freemani reference genome and the complete mitogenome presented here represent useful resources for comparative evolutionary studies of related species and for further basic and applied research on different biological aspects of economically important pests. MDPI 2022-05-24 /pmc/articles/PMC9180572/ /pubmed/35682551 http://dx.doi.org/10.3390/ijms23115869 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Volarić, Marin Despot-Slade, Evelin Veseljak, Damira Meštrović, Nevenka Mravinac, Brankica Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title | Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title_full | Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title_fullStr | Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title_full_unstemmed | Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title_short | Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani |
title_sort | reference-guided de novo genome assembly of the flour beetle tribolium freemani |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180572/ https://www.ncbi.nlm.nih.gov/pubmed/35682551 http://dx.doi.org/10.3390/ijms23115869 |
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