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Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin

Sarcopenia is a gradual and generalized skeletal muscle (SKM) syndrome, characterized by the impairment of muscle components and functionality. Hydrogen sulfide (H(2)S), endogenously formed within the body from the activity of cystathionine-γ-lyase (CSE), cystathionine- β-synthase (CBS), and mercapt...

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Autores principales: Micheli, Laura, Mitidieri, Emma, Turnaturi, Carlotta, Vanacore, Domenico, Ciampi, Clara, Lucarini, Elena, Cirino, Giuseppe, Ghelardini, Carla, Sorrentino, Raffaella, Di Cesare Mannelli, Lorenzo, d’Emmanuele di Villa Bianca, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180606/
https://www.ncbi.nlm.nih.gov/pubmed/35682634
http://dx.doi.org/10.3390/ijms23115955
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author Micheli, Laura
Mitidieri, Emma
Turnaturi, Carlotta
Vanacore, Domenico
Ciampi, Clara
Lucarini, Elena
Cirino, Giuseppe
Ghelardini, Carla
Sorrentino, Raffaella
Di Cesare Mannelli, Lorenzo
d’Emmanuele di Villa Bianca, Roberta
author_facet Micheli, Laura
Mitidieri, Emma
Turnaturi, Carlotta
Vanacore, Domenico
Ciampi, Clara
Lucarini, Elena
Cirino, Giuseppe
Ghelardini, Carla
Sorrentino, Raffaella
Di Cesare Mannelli, Lorenzo
d’Emmanuele di Villa Bianca, Roberta
author_sort Micheli, Laura
collection PubMed
description Sarcopenia is a gradual and generalized skeletal muscle (SKM) syndrome, characterized by the impairment of muscle components and functionality. Hydrogen sulfide (H(2)S), endogenously formed within the body from the activity of cystathionine-γ-lyase (CSE), cystathionine- β-synthase (CBS), and mercaptopyruvate sulfurtransferase, is involved in SKM function. Here, in an in vitro model of sarcopenia based on damage induced by dexamethasone (DEX, 1 μM, 48 h treatment) in C2C12-derived myotubes, we investigated the protective potential of exogenous and endogenous sources of H(2)S, i.e., glucoraphanin (30 μM), L-cysteine (150 μM), and 3-mercaptopyruvate (150 μM). DEX impaired the H(2)S signalling in terms of a reduction in CBS and CSE expression and H(2)S biosynthesis. Glucoraphanin and 3-mercaptopyruvate but not L-cysteine prevented the apoptotic process induced by DEX. In parallel, the H(2)S-releasing molecules reduced the oxidative unbalance evoked by DEX, reducing catalase activity, O(2)(−) levels, and protein carbonylation. Glucoraphanin, 3-mercaptopyruvate, and L-cysteine avoided the changes in myotubes morphology and morphometrics after DEX treatment. In conclusion, in an in vitro model of sarcopenia, an impairment in CBS/CSE/H(2)S signalling occurs, whereas glucoraphanin, a natural H(2)S-releasing molecule, appears more effective for preventing the SKM damage. Therefore, glucoraphanin supplementation could be an innovative therapeutic approach in the management of sarcopenia.
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spelling pubmed-91806062022-06-10 Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin Micheli, Laura Mitidieri, Emma Turnaturi, Carlotta Vanacore, Domenico Ciampi, Clara Lucarini, Elena Cirino, Giuseppe Ghelardini, Carla Sorrentino, Raffaella Di Cesare Mannelli, Lorenzo d’Emmanuele di Villa Bianca, Roberta Int J Mol Sci Article Sarcopenia is a gradual and generalized skeletal muscle (SKM) syndrome, characterized by the impairment of muscle components and functionality. Hydrogen sulfide (H(2)S), endogenously formed within the body from the activity of cystathionine-γ-lyase (CSE), cystathionine- β-synthase (CBS), and mercaptopyruvate sulfurtransferase, is involved in SKM function. Here, in an in vitro model of sarcopenia based on damage induced by dexamethasone (DEX, 1 μM, 48 h treatment) in C2C12-derived myotubes, we investigated the protective potential of exogenous and endogenous sources of H(2)S, i.e., glucoraphanin (30 μM), L-cysteine (150 μM), and 3-mercaptopyruvate (150 μM). DEX impaired the H(2)S signalling in terms of a reduction in CBS and CSE expression and H(2)S biosynthesis. Glucoraphanin and 3-mercaptopyruvate but not L-cysteine prevented the apoptotic process induced by DEX. In parallel, the H(2)S-releasing molecules reduced the oxidative unbalance evoked by DEX, reducing catalase activity, O(2)(−) levels, and protein carbonylation. Glucoraphanin, 3-mercaptopyruvate, and L-cysteine avoided the changes in myotubes morphology and morphometrics after DEX treatment. In conclusion, in an in vitro model of sarcopenia, an impairment in CBS/CSE/H(2)S signalling occurs, whereas glucoraphanin, a natural H(2)S-releasing molecule, appears more effective for preventing the SKM damage. Therefore, glucoraphanin supplementation could be an innovative therapeutic approach in the management of sarcopenia. MDPI 2022-05-25 /pmc/articles/PMC9180606/ /pubmed/35682634 http://dx.doi.org/10.3390/ijms23115955 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Micheli, Laura
Mitidieri, Emma
Turnaturi, Carlotta
Vanacore, Domenico
Ciampi, Clara
Lucarini, Elena
Cirino, Giuseppe
Ghelardini, Carla
Sorrentino, Raffaella
Di Cesare Mannelli, Lorenzo
d’Emmanuele di Villa Bianca, Roberta
Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title_full Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title_fullStr Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title_full_unstemmed Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title_short Beneficial Effect of H(2)S-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin
title_sort beneficial effect of h(2)s-releasing molecules in an in vitro model of sarcopenia: relevance of glucoraphanin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180606/
https://www.ncbi.nlm.nih.gov/pubmed/35682634
http://dx.doi.org/10.3390/ijms23115955
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