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The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis

The expression pattern of Connexins (Cx) 37, 40, 43, 45 and Pannexin 1 (Pnx1) was analyzed immunohistochemically, as well as semi-quantitatively and quantitatively in histological sections of developing 8th- to 12th-week human eyes and postnatal healthy eye, in retinoblastoma and different uveal mel...

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Autores principales: Žužul, Matea, Lozić, Mirela, Filipović, Natalija, Čanović, Samir, Didović Pavičić, Ana, Petričević, Joško, Kunac, Nenad, Šoljić, Violeta, Saraga-Babić, Mirna, Konjevoda, Suzana, Vukojevic, Katarina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180640/
https://www.ncbi.nlm.nih.gov/pubmed/35682601
http://dx.doi.org/10.3390/ijms23115918
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author Žužul, Matea
Lozić, Mirela
Filipović, Natalija
Čanović, Samir
Didović Pavičić, Ana
Petričević, Joško
Kunac, Nenad
Šoljić, Violeta
Saraga-Babić, Mirna
Konjevoda, Suzana
Vukojevic, Katarina
author_facet Žužul, Matea
Lozić, Mirela
Filipović, Natalija
Čanović, Samir
Didović Pavičić, Ana
Petričević, Joško
Kunac, Nenad
Šoljić, Violeta
Saraga-Babić, Mirna
Konjevoda, Suzana
Vukojevic, Katarina
author_sort Žužul, Matea
collection PubMed
description The expression pattern of Connexins (Cx) 37, 40, 43, 45 and Pannexin 1 (Pnx1) was analyzed immunohistochemically, as well as semi-quantitatively and quantitatively in histological sections of developing 8th- to 12th-week human eyes and postnatal healthy eye, in retinoblastoma and different uveal melanomas. Expressions of both Cx37 and Cx43 increased during development but diminished in the postnatal period, being higher in the retina than in the choroid. Cx37 was highly expressed in the choroid of retinoblastoma, and Cx43 in epitheloid melanoma, while they were both increasingly expressed in mixoid melanoma. In contrast, mild retinal Cx40 expression during development increased to strong in postnatal period, while it was significantly higher in the choroid of mixoid melanoma. Cx45 showed significantly higher expression in the developing retina compared to other samples, while it became low postnatally and in all types of melanoma. Pnx1 was increasingly expressed in developing choroid but became lower in the postnatal eye. It was strongly expressed in epithelial and spindle melanoma, and particularly in retinoblastoma. Our results indicate importance of Cx37 and Cx40 expression in normal and pathological vascularization, and Cx43 expression in inflammatory response. Whereas Cx45 is involved in early stages of eye development, Pnx1might influence cell metabolism. Additionally, Cx43 might be a potential biomarker of tumor prognosis.
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spelling pubmed-91806402022-06-10 The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis Žužul, Matea Lozić, Mirela Filipović, Natalija Čanović, Samir Didović Pavičić, Ana Petričević, Joško Kunac, Nenad Šoljić, Violeta Saraga-Babić, Mirna Konjevoda, Suzana Vukojevic, Katarina Int J Mol Sci Article The expression pattern of Connexins (Cx) 37, 40, 43, 45 and Pannexin 1 (Pnx1) was analyzed immunohistochemically, as well as semi-quantitatively and quantitatively in histological sections of developing 8th- to 12th-week human eyes and postnatal healthy eye, in retinoblastoma and different uveal melanomas. Expressions of both Cx37 and Cx43 increased during development but diminished in the postnatal period, being higher in the retina than in the choroid. Cx37 was highly expressed in the choroid of retinoblastoma, and Cx43 in epitheloid melanoma, while they were both increasingly expressed in mixoid melanoma. In contrast, mild retinal Cx40 expression during development increased to strong in postnatal period, while it was significantly higher in the choroid of mixoid melanoma. Cx45 showed significantly higher expression in the developing retina compared to other samples, while it became low postnatally and in all types of melanoma. Pnx1 was increasingly expressed in developing choroid but became lower in the postnatal eye. It was strongly expressed in epithelial and spindle melanoma, and particularly in retinoblastoma. Our results indicate importance of Cx37 and Cx40 expression in normal and pathological vascularization, and Cx43 expression in inflammatory response. Whereas Cx45 is involved in early stages of eye development, Pnx1might influence cell metabolism. Additionally, Cx43 might be a potential biomarker of tumor prognosis. MDPI 2022-05-25 /pmc/articles/PMC9180640/ /pubmed/35682601 http://dx.doi.org/10.3390/ijms23115918 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Žužul, Matea
Lozić, Mirela
Filipović, Natalija
Čanović, Samir
Didović Pavičić, Ana
Petričević, Joško
Kunac, Nenad
Šoljić, Violeta
Saraga-Babić, Mirna
Konjevoda, Suzana
Vukojevic, Katarina
The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title_full The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title_fullStr The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title_full_unstemmed The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title_short The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis
title_sort expression of connexin 37, 40, 43, 45 and pannexin 1 in the early human retina and choroid development and tumorigenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180640/
https://www.ncbi.nlm.nih.gov/pubmed/35682601
http://dx.doi.org/10.3390/ijms23115918
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