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Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA...

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Autores principales: Lu, Qianqian, Guo, Yingqi, Yang, Guo, Cui, Lei, Wu, Zhen, Zeng, Xiaoqun, Pan, Daodong, Cai, Zhendong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180668/
https://www.ncbi.nlm.nih.gov/pubmed/35681360
http://dx.doi.org/10.3390/foods11111610
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author Lu, Qianqian
Guo, Yingqi
Yang, Guo
Cui, Lei
Wu, Zhen
Zeng, Xiaoqun
Pan, Daodong
Cai, Zhendong
author_facet Lu, Qianqian
Guo, Yingqi
Yang, Guo
Cui, Lei
Wu, Zhen
Zeng, Xiaoqun
Pan, Daodong
Cai, Zhendong
author_sort Lu, Qianqian
collection PubMed
description Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from Lactobacillus plantarum A3, Lactobacillus reuteri DMSZ 8533, and Lactobacillus acidophilus CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of Lactobacillus strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three Lactobacillus strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that L. reuteri LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment.
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spelling pubmed-91806682022-06-10 Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains Lu, Qianqian Guo, Yingqi Yang, Guo Cui, Lei Wu, Zhen Zeng, Xiaoqun Pan, Daodong Cai, Zhendong Foods Article Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from Lactobacillus plantarum A3, Lactobacillus reuteri DMSZ 8533, and Lactobacillus acidophilus CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of Lactobacillus strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three Lactobacillus strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that L. reuteri LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment. MDPI 2022-05-30 /pmc/articles/PMC9180668/ /pubmed/35681360 http://dx.doi.org/10.3390/foods11111610 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Qianqian
Guo, Yingqi
Yang, Guo
Cui, Lei
Wu, Zhen
Zeng, Xiaoqun
Pan, Daodong
Cai, Zhendong
Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title_full Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title_fullStr Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title_full_unstemmed Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title_short Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
title_sort structure and anti-inflammation potential of lipoteichoic acids isolated from lactobacillus strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180668/
https://www.ncbi.nlm.nih.gov/pubmed/35681360
http://dx.doi.org/10.3390/foods11111610
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