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Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180668/ https://www.ncbi.nlm.nih.gov/pubmed/35681360 http://dx.doi.org/10.3390/foods11111610 |
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author | Lu, Qianqian Guo, Yingqi Yang, Guo Cui, Lei Wu, Zhen Zeng, Xiaoqun Pan, Daodong Cai, Zhendong |
author_facet | Lu, Qianqian Guo, Yingqi Yang, Guo Cui, Lei Wu, Zhen Zeng, Xiaoqun Pan, Daodong Cai, Zhendong |
author_sort | Lu, Qianqian |
collection | PubMed |
description | Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from Lactobacillus plantarum A3, Lactobacillus reuteri DMSZ 8533, and Lactobacillus acidophilus CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of Lactobacillus strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three Lactobacillus strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that L. reuteri LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment. |
format | Online Article Text |
id | pubmed-9180668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91806682022-06-10 Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains Lu, Qianqian Guo, Yingqi Yang, Guo Cui, Lei Wu, Zhen Zeng, Xiaoqun Pan, Daodong Cai, Zhendong Foods Article Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from Lactobacillus plantarum A3, Lactobacillus reuteri DMSZ 8533, and Lactobacillus acidophilus CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of Lactobacillus strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three Lactobacillus strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that L. reuteri LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment. MDPI 2022-05-30 /pmc/articles/PMC9180668/ /pubmed/35681360 http://dx.doi.org/10.3390/foods11111610 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lu, Qianqian Guo, Yingqi Yang, Guo Cui, Lei Wu, Zhen Zeng, Xiaoqun Pan, Daodong Cai, Zhendong Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title_full | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title_fullStr | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title_full_unstemmed | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title_short | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains |
title_sort | structure and anti-inflammation potential of lipoteichoic acids isolated from lactobacillus strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180668/ https://www.ncbi.nlm.nih.gov/pubmed/35681360 http://dx.doi.org/10.3390/foods11111610 |
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