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Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans
For this investigation, Caenorhabditis elegans (C. elegans) served, for the first time, as a model organism to evaluate the toxic effect and possible underlying mechanisms under acrolein (ACR) exposure. The results showed that ACR exposure (12.5–100 μM) shortened the lifespan of C. elegans. The repr...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180825/ https://www.ncbi.nlm.nih.gov/pubmed/35681340 http://dx.doi.org/10.3390/foods11111590 |
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author | Hong, Jiaqian Song, Yiming Xie, Jiayan Xie, Jianhua Chen, Yi Li, Ping Liu, Danyang Hu, Xiaobo Yu, Qiang |
author_facet | Hong, Jiaqian Song, Yiming Xie, Jiayan Xie, Jianhua Chen, Yi Li, Ping Liu, Danyang Hu, Xiaobo Yu, Qiang |
author_sort | Hong, Jiaqian |
collection | PubMed |
description | For this investigation, Caenorhabditis elegans (C. elegans) served, for the first time, as a model organism to evaluate the toxic effect and possible underlying mechanisms under acrolein (ACR) exposure. The results showed that ACR exposure (12.5–100 μM) shortened the lifespan of C. elegans. The reproductive capacity, body length, body width, and locomotive behavior (head thrash) of C. elegans were diminished by ACR, especially the doses of 50 and 100 μM. Furthermore, ACR significantly enhanced the endogenous ROS levels of C. elegans, inhibited the antioxidant-related enzyme activities, and affected the expression of antioxidant related genes. The increasing oxidative stress level promoted the migration of DAF-16 into the nucleus that was related to the DAF-16/FOXO pathway. It was also confirmed by the significant decrease of the lifespan-shortening trend in the daf-16 knockout mutant. In conclusion, ACR exposure induced aging and oxidative stress in C. elegans, resulting in aging-related decline and defense-related DAF-16/FOXO pathways’ activation. |
format | Online Article Text |
id | pubmed-9180825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91808252022-06-10 Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans Hong, Jiaqian Song, Yiming Xie, Jiayan Xie, Jianhua Chen, Yi Li, Ping Liu, Danyang Hu, Xiaobo Yu, Qiang Foods Article For this investigation, Caenorhabditis elegans (C. elegans) served, for the first time, as a model organism to evaluate the toxic effect and possible underlying mechanisms under acrolein (ACR) exposure. The results showed that ACR exposure (12.5–100 μM) shortened the lifespan of C. elegans. The reproductive capacity, body length, body width, and locomotive behavior (head thrash) of C. elegans were diminished by ACR, especially the doses of 50 and 100 μM. Furthermore, ACR significantly enhanced the endogenous ROS levels of C. elegans, inhibited the antioxidant-related enzyme activities, and affected the expression of antioxidant related genes. The increasing oxidative stress level promoted the migration of DAF-16 into the nucleus that was related to the DAF-16/FOXO pathway. It was also confirmed by the significant decrease of the lifespan-shortening trend in the daf-16 knockout mutant. In conclusion, ACR exposure induced aging and oxidative stress in C. elegans, resulting in aging-related decline and defense-related DAF-16/FOXO pathways’ activation. MDPI 2022-05-28 /pmc/articles/PMC9180825/ /pubmed/35681340 http://dx.doi.org/10.3390/foods11111590 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hong, Jiaqian Song, Yiming Xie, Jiayan Xie, Jianhua Chen, Yi Li, Ping Liu, Danyang Hu, Xiaobo Yu, Qiang Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title | Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title_full | Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title_fullStr | Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title_full_unstemmed | Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title_short | Acrolein Promotes Aging and Oxidative Stress via the Stress Response Factor DAF-16/FOXO in Caenorhabditis elegans |
title_sort | acrolein promotes aging and oxidative stress via the stress response factor daf-16/foxo in caenorhabditis elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180825/ https://www.ncbi.nlm.nih.gov/pubmed/35681340 http://dx.doi.org/10.3390/foods11111590 |
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