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The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize
Progress through the cell cycle is a critical process during plant embryo and seed development and its progression is regulated by cyclins. Despite extensive study of cyclins in other systems, their role in embryo and seed development of maize is unclear. In this study, we demonstrate that ZmCYCB1-1...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180882/ https://www.ncbi.nlm.nih.gov/pubmed/35682593 http://dx.doi.org/10.3390/ijms23115907 |
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author | Zhao, Bingbing Zhou, Miaoyi Ren, Wen Li, Hanshuai Zhang, Qian He, Guangming Liu, Ya He, Haohua |
author_facet | Zhao, Bingbing Zhou, Miaoyi Ren, Wen Li, Hanshuai Zhang, Qian He, Guangming Liu, Ya He, Haohua |
author_sort | Zhao, Bingbing |
collection | PubMed |
description | Progress through the cell cycle is a critical process during plant embryo and seed development and its progression is regulated by cyclins. Despite extensive study of cyclins in other systems, their role in embryo and seed development of maize is unclear. In this study, we demonstrate that ZmCYCB1-1 overexpression significantly accelerated embryo growth and increased seed size. In situ hybridization and toluidine blue staining indicated that ZmCYCB1-1 was highly expressed in the plumule of embryos, and the cells of the plumule were smaller, denser, and more regularly arranged in ZmCYCB1-1 overexpression plants. Overexpression of ZmCYCB1-1 in maize also resulted in an increased ear length and enhanced kernel weight by increasing kernel width. Transcriptome analysis indicated that the overexpression of ZmCYCB1-1 affected several different metabolic pathways, including photosynthesis in embryos and leaves, and lipid metabolism in leaves. Conversely, knocking out ZmCYCB1-1 resulted in plants with slow growth. Our results suggest that ZmCYCB1-1 regulates embryo growth and seed size, making it an ideal target for efforts aimed at maize yield improvement. |
format | Online Article Text |
id | pubmed-9180882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91808822022-06-10 The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize Zhao, Bingbing Zhou, Miaoyi Ren, Wen Li, Hanshuai Zhang, Qian He, Guangming Liu, Ya He, Haohua Int J Mol Sci Article Progress through the cell cycle is a critical process during plant embryo and seed development and its progression is regulated by cyclins. Despite extensive study of cyclins in other systems, their role in embryo and seed development of maize is unclear. In this study, we demonstrate that ZmCYCB1-1 overexpression significantly accelerated embryo growth and increased seed size. In situ hybridization and toluidine blue staining indicated that ZmCYCB1-1 was highly expressed in the plumule of embryos, and the cells of the plumule were smaller, denser, and more regularly arranged in ZmCYCB1-1 overexpression plants. Overexpression of ZmCYCB1-1 in maize also resulted in an increased ear length and enhanced kernel weight by increasing kernel width. Transcriptome analysis indicated that the overexpression of ZmCYCB1-1 affected several different metabolic pathways, including photosynthesis in embryos and leaves, and lipid metabolism in leaves. Conversely, knocking out ZmCYCB1-1 resulted in plants with slow growth. Our results suggest that ZmCYCB1-1 regulates embryo growth and seed size, making it an ideal target for efforts aimed at maize yield improvement. MDPI 2022-05-25 /pmc/articles/PMC9180882/ /pubmed/35682593 http://dx.doi.org/10.3390/ijms23115907 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Bingbing Zhou, Miaoyi Ren, Wen Li, Hanshuai Zhang, Qian He, Guangming Liu, Ya He, Haohua The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title | The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title_full | The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title_fullStr | The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title_full_unstemmed | The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title_short | The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize |
title_sort | b-type cyclin cycb1-1 regulates embryonic development and seed size in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180882/ https://www.ncbi.nlm.nih.gov/pubmed/35682593 http://dx.doi.org/10.3390/ijms23115907 |
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