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Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor
Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reacti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180964/ https://www.ncbi.nlm.nih.gov/pubmed/35683005 http://dx.doi.org/10.3390/ijms23116326 |
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author | Sun, Ting Li, Xian Jin, Xiaochuan Wu, Ziyi Chen, Xiachao Qiu, Jieqiong |
author_facet | Sun, Ting Li, Xian Jin, Xiaochuan Wu, Ziyi Chen, Xiachao Qiu, Jieqiong |
author_sort | Sun, Ting |
collection | PubMed |
description | Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg(2+) detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg(2+)–T when Hg(2+) is present, and no fluorescence is observed. It is digested by exonuclease I without Hg(2+), and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg(2+) concentration in the range of 0–250 nM (R(2) = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg(2+) detection, obtaining a recovery rate from 99.6% to 101.1%. |
format | Online Article Text |
id | pubmed-9180964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91809642022-06-10 Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor Sun, Ting Li, Xian Jin, Xiaochuan Wu, Ziyi Chen, Xiachao Qiu, Jieqiong Int J Mol Sci Article Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg(2+) detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg(2+)–T when Hg(2+) is present, and no fluorescence is observed. It is digested by exonuclease I without Hg(2+), and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg(2+) concentration in the range of 0–250 nM (R(2) = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg(2+) detection, obtaining a recovery rate from 99.6% to 101.1%. MDPI 2022-06-05 /pmc/articles/PMC9180964/ /pubmed/35683005 http://dx.doi.org/10.3390/ijms23116326 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sun, Ting Li, Xian Jin, Xiaochuan Wu, Ziyi Chen, Xiachao Qiu, Jieqiong Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title | Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title_full | Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title_fullStr | Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title_full_unstemmed | Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title_short | Function of Graphene Oxide as the “Nanoquencher” for Hg(2+) Detection Using an Exonuclease I-Assisted Biosensor |
title_sort | function of graphene oxide as the “nanoquencher” for hg(2+) detection using an exonuclease i-assisted biosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180964/ https://www.ncbi.nlm.nih.gov/pubmed/35683005 http://dx.doi.org/10.3390/ijms23116326 |
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