Cargando…

Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid

The Gastric pathogen Helicobacter pylori (HP) may influence the development of coronary heart disease (CHD). H. pylori induce reactive oxygen species (ROS), which transform cholesterol to 7-ketocholesterol (7-kCh), a CHD risk factor. Acetylsalicylic acid (ASA)—an Anti-aggregation drug used in CHD pa...

Descripción completa

Detalles Bibliográficos
Autores principales: Gajewski, Adrian Ł., Gawrysiak, Mateusz, Krupa, Agnieszka, Rechciński, Tomasz, Chałubiński, Maciej, Gonciarz, Weronika, Chmiela, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181086/
https://www.ncbi.nlm.nih.gov/pubmed/35683034
http://dx.doi.org/10.3390/ijms23116355
_version_ 1784723681005535232
author Gajewski, Adrian Ł.
Gawrysiak, Mateusz
Krupa, Agnieszka
Rechciński, Tomasz
Chałubiński, Maciej
Gonciarz, Weronika
Chmiela, Magdalena
author_facet Gajewski, Adrian Ł.
Gawrysiak, Mateusz
Krupa, Agnieszka
Rechciński, Tomasz
Chałubiński, Maciej
Gonciarz, Weronika
Chmiela, Magdalena
author_sort Gajewski, Adrian Ł.
collection PubMed
description The Gastric pathogen Helicobacter pylori (HP) may influence the development of coronary heart disease (CHD). H. pylori induce reactive oxygen species (ROS), which transform cholesterol to 7-ketocholesterol (7-kCh), a CHD risk factor. Acetylsalicylic acid (ASA)—an Anti-aggregation drug used in CHD patients—may increase gastric bleeding and inflammation. We examined whether H. pylori driven ROS effects in the cell cultures of gastric epithelial cells (AGS) and vascular endothelial cells (HUVEC) progress in the milieu of 7-kCh and ASA. Cell cultures, exposed to 7-kCh or ASA alone or pulsed with the H. pylori antigenic complex—Glycine acid extract (GE), urease (UreA), cytotoxin associated gene A (CagA) protein or lipopolysaccharide (LPS), alone or with 7-kCh and ASA—were examined for ROS, apoptosis, cell integrity, interleukin (IL)-8, the activation of signal transducer, the activator of transcription 3 (STAT3), and wound healing. ASA and 7-kCh alone, and particularly in conjunction with H. pylori components, increased the ROS level and the rate of apoptosis, which was followed by cell disintegration, the activation of STAT3, and IL-8 elevation. AGS cells were unable to undergo wound healing. The cell ROS response to H. pylori components may be elevated by 7-kCh and ASA.
format Online
Article
Text
id pubmed-9181086
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91810862022-06-10 Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid Gajewski, Adrian Ł. Gawrysiak, Mateusz Krupa, Agnieszka Rechciński, Tomasz Chałubiński, Maciej Gonciarz, Weronika Chmiela, Magdalena Int J Mol Sci Article The Gastric pathogen Helicobacter pylori (HP) may influence the development of coronary heart disease (CHD). H. pylori induce reactive oxygen species (ROS), which transform cholesterol to 7-ketocholesterol (7-kCh), a CHD risk factor. Acetylsalicylic acid (ASA)—an Anti-aggregation drug used in CHD patients—may increase gastric bleeding and inflammation. We examined whether H. pylori driven ROS effects in the cell cultures of gastric epithelial cells (AGS) and vascular endothelial cells (HUVEC) progress in the milieu of 7-kCh and ASA. Cell cultures, exposed to 7-kCh or ASA alone or pulsed with the H. pylori antigenic complex—Glycine acid extract (GE), urease (UreA), cytotoxin associated gene A (CagA) protein or lipopolysaccharide (LPS), alone or with 7-kCh and ASA—were examined for ROS, apoptosis, cell integrity, interleukin (IL)-8, the activation of signal transducer, the activator of transcription 3 (STAT3), and wound healing. ASA and 7-kCh alone, and particularly in conjunction with H. pylori components, increased the ROS level and the rate of apoptosis, which was followed by cell disintegration, the activation of STAT3, and IL-8 elevation. AGS cells were unable to undergo wound healing. The cell ROS response to H. pylori components may be elevated by 7-kCh and ASA. MDPI 2022-06-06 /pmc/articles/PMC9181086/ /pubmed/35683034 http://dx.doi.org/10.3390/ijms23116355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gajewski, Adrian Ł.
Gawrysiak, Mateusz
Krupa, Agnieszka
Rechciński, Tomasz
Chałubiński, Maciej
Gonciarz, Weronika
Chmiela, Magdalena
Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title_full Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title_fullStr Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title_full_unstemmed Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title_short Accumulation of Deleterious Effects in Gastric Epithelial Cells and Vascular Endothelial Cells In Vitro in the Milieu of Helicobacter pylori Components, 7-Ketocholesterol and Acetylsalicylic Acid
title_sort accumulation of deleterious effects in gastric epithelial cells and vascular endothelial cells in vitro in the milieu of helicobacter pylori components, 7-ketocholesterol and acetylsalicylic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181086/
https://www.ncbi.nlm.nih.gov/pubmed/35683034
http://dx.doi.org/10.3390/ijms23116355
work_keys_str_mv AT gajewskiadrianł accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT gawrysiakmateusz accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT krupaagnieszka accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT rechcinskitomasz accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT chałubinskimaciej accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT gonciarzweronika accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid
AT chmielamagdalena accumulationofdeleteriouseffectsingastricepithelialcellsandvascularendothelialcellsinvitrointhemilieuofhelicobacterpyloricomponents7ketocholesterolandacetylsalicylicacid