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Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue

Herpes simplex virus type I (HSV-1) infection is a potential risk factor involved in the Amyloid β (Aβ) associated neuropathology. However, further understanding of the neuropathological effects of the HSV-1 infection is hampered by the limitations of existing infection models due to the distinct di...

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Autores principales: Qiao, Haowen, Zhao, Wen, Guo, Moujian, Zhu, Lili, Chen, Tao, Wang, Jibo, Xu, Xiaodong, Zhang, Zhentao, Wu, Ying, Chen, Pu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181143/
https://www.ncbi.nlm.nih.gov/pubmed/35682661
http://dx.doi.org/10.3390/ijms23115981
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author Qiao, Haowen
Zhao, Wen
Guo, Moujian
Zhu, Lili
Chen, Tao
Wang, Jibo
Xu, Xiaodong
Zhang, Zhentao
Wu, Ying
Chen, Pu
author_facet Qiao, Haowen
Zhao, Wen
Guo, Moujian
Zhu, Lili
Chen, Tao
Wang, Jibo
Xu, Xiaodong
Zhang, Zhentao
Wu, Ying
Chen, Pu
author_sort Qiao, Haowen
collection PubMed
description Herpes simplex virus type I (HSV-1) infection is a potential risk factor involved in the Amyloid β (Aβ) associated neuropathology. However, further understanding of the neuropathological effects of the HSV-1 infection is hampered by the limitations of existing infection models due to the distinct differences between human brains and other mammalians’ brains. Here we generated cerebral organoid models derived from pluripotent stem cells to investigate the HSV-induced Aβ associated neuropathology and the role of antiviral drugs in the phenotypic rescue. Our results identified that the HSV-1-infected cerebral organoids recapitulated Aβ associated neuropathology including the multicellular Aβ deposition, dysregulated endogenous AD mediators, reactive gliosis, neuroinflammation, and neural loss, indicating that cerebral organoids offer an opportunity for modeling the interaction of HSV-1 with the complex phenotypes across the genetic, cellular, and tissue levels of the human Alzheimer’s disease (AD). Furthermore, we identified that two antiviral drugs, namely Ribavirin (RBV) and Valacyclovir (VCV), inhibited HSV-1 replication and rescued the neuropathological phenotypes associated with AD in the HSV-1-infected cerebral organoids, implying their therapeutic potential to slow down the progression of AD. Our study provides a high-fidelity human-relevant in-vitro HSV-1 infection model to reconstitute the multiscale neuropathological features associated with AD and discover therapeutic drug candidates relevant to the AD viral hypothesis.
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spelling pubmed-91811432022-06-10 Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue Qiao, Haowen Zhao, Wen Guo, Moujian Zhu, Lili Chen, Tao Wang, Jibo Xu, Xiaodong Zhang, Zhentao Wu, Ying Chen, Pu Int J Mol Sci Article Herpes simplex virus type I (HSV-1) infection is a potential risk factor involved in the Amyloid β (Aβ) associated neuropathology. However, further understanding of the neuropathological effects of the HSV-1 infection is hampered by the limitations of existing infection models due to the distinct differences between human brains and other mammalians’ brains. Here we generated cerebral organoid models derived from pluripotent stem cells to investigate the HSV-induced Aβ associated neuropathology and the role of antiviral drugs in the phenotypic rescue. Our results identified that the HSV-1-infected cerebral organoids recapitulated Aβ associated neuropathology including the multicellular Aβ deposition, dysregulated endogenous AD mediators, reactive gliosis, neuroinflammation, and neural loss, indicating that cerebral organoids offer an opportunity for modeling the interaction of HSV-1 with the complex phenotypes across the genetic, cellular, and tissue levels of the human Alzheimer’s disease (AD). Furthermore, we identified that two antiviral drugs, namely Ribavirin (RBV) and Valacyclovir (VCV), inhibited HSV-1 replication and rescued the neuropathological phenotypes associated with AD in the HSV-1-infected cerebral organoids, implying their therapeutic potential to slow down the progression of AD. Our study provides a high-fidelity human-relevant in-vitro HSV-1 infection model to reconstitute the multiscale neuropathological features associated with AD and discover therapeutic drug candidates relevant to the AD viral hypothesis. MDPI 2022-05-26 /pmc/articles/PMC9181143/ /pubmed/35682661 http://dx.doi.org/10.3390/ijms23115981 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiao, Haowen
Zhao, Wen
Guo, Moujian
Zhu, Lili
Chen, Tao
Wang, Jibo
Xu, Xiaodong
Zhang, Zhentao
Wu, Ying
Chen, Pu
Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title_full Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title_fullStr Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title_full_unstemmed Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title_short Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue
title_sort cerebral organoids for modeling of hsv-1-induced-amyloid β associated neuropathology and phenotypic rescue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181143/
https://www.ncbi.nlm.nih.gov/pubmed/35682661
http://dx.doi.org/10.3390/ijms23115981
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