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Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori

Bombyx mori nucleopolyhedrovirus (BmNPV) causes major economic losses in sericulture. A number of agents have been employed to treat viral diseases. Silver nanoparticles (AgNPs) have wide applications in biomedical fields due to their unique properties. The anti-BmNPV effect of AgNPs has been evalua...

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Autores principales: Deng, Boyuan, Dong, Zhanqi, Wu, Qin, Guo, Bingyu, Fang, Wenxuan, Hu, Congwu, Long, Jiangqiong, Chen, Peng, Lu, Cheng, Pan, Minhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181153/
https://www.ncbi.nlm.nih.gov/pubmed/35683003
http://dx.doi.org/10.3390/ijms23116325
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author Deng, Boyuan
Dong, Zhanqi
Wu, Qin
Guo, Bingyu
Fang, Wenxuan
Hu, Congwu
Long, Jiangqiong
Chen, Peng
Lu, Cheng
Pan, Minhui
author_facet Deng, Boyuan
Dong, Zhanqi
Wu, Qin
Guo, Bingyu
Fang, Wenxuan
Hu, Congwu
Long, Jiangqiong
Chen, Peng
Lu, Cheng
Pan, Minhui
author_sort Deng, Boyuan
collection PubMed
description Bombyx mori nucleopolyhedrovirus (BmNPV) causes major economic losses in sericulture. A number of agents have been employed to treat viral diseases. Silver nanoparticles (AgNPs) have wide applications in biomedical fields due to their unique properties. The anti-BmNPV effect of AgNPs has been evaluated, however, there are insufficient studies concerning its toxicity to other organisms and the environment. We chemically synthesized biocompatible BSA-AgNPs with a diameter range of 2–4 nm and characterized their physical properties. The toxicity of AgNPs towards cells and larvae with different concentrations was examined; the results indicated a biofriendly effect on cells and larvae within specific concentration ranges. The SEM observation of the surface of BmNPV after treatment with AgNPs suggested that AgNPs could destroy the polyhedral structure, and the same result was obtained by Coomassie blue staining. Further assays confirmed the weakened virulence of AgNPs-treated BmNPV toward cells and larvae. AgNPs also could effectively inhibit the replication of BmNPV in infected cells and larvae. In summary, our research provides valuable data for the further development of AgNPs as an antiviral drug for sericulture.
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spelling pubmed-91811532022-06-10 Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori Deng, Boyuan Dong, Zhanqi Wu, Qin Guo, Bingyu Fang, Wenxuan Hu, Congwu Long, Jiangqiong Chen, Peng Lu, Cheng Pan, Minhui Int J Mol Sci Article Bombyx mori nucleopolyhedrovirus (BmNPV) causes major economic losses in sericulture. A number of agents have been employed to treat viral diseases. Silver nanoparticles (AgNPs) have wide applications in biomedical fields due to their unique properties. The anti-BmNPV effect of AgNPs has been evaluated, however, there are insufficient studies concerning its toxicity to other organisms and the environment. We chemically synthesized biocompatible BSA-AgNPs with a diameter range of 2–4 nm and characterized their physical properties. The toxicity of AgNPs towards cells and larvae with different concentrations was examined; the results indicated a biofriendly effect on cells and larvae within specific concentration ranges. The SEM observation of the surface of BmNPV after treatment with AgNPs suggested that AgNPs could destroy the polyhedral structure, and the same result was obtained by Coomassie blue staining. Further assays confirmed the weakened virulence of AgNPs-treated BmNPV toward cells and larvae. AgNPs also could effectively inhibit the replication of BmNPV in infected cells and larvae. In summary, our research provides valuable data for the further development of AgNPs as an antiviral drug for sericulture. MDPI 2022-06-05 /pmc/articles/PMC9181153/ /pubmed/35683003 http://dx.doi.org/10.3390/ijms23116325 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deng, Boyuan
Dong, Zhanqi
Wu, Qin
Guo, Bingyu
Fang, Wenxuan
Hu, Congwu
Long, Jiangqiong
Chen, Peng
Lu, Cheng
Pan, Minhui
Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title_full Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title_fullStr Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title_full_unstemmed Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title_short Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting Bombyx mori
title_sort analysis of silver nanoparticles for the treatment and prevention of nucleopolyhedrovirus affecting bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181153/
https://www.ncbi.nlm.nih.gov/pubmed/35683003
http://dx.doi.org/10.3390/ijms23116325
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