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RadA, a Key Gene of the Circadian Rhythm of Escherichia coli

Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformat...

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Autores principales: Diallo, Aissatou Bailo, Mezouar, Soraya, Boumaza, Asma, Fiammingo, Oksana, Coiffard, Benjamin, Pontarotti, Pierre, Desnues, Benoit, Mege, Jean-Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181324/
https://www.ncbi.nlm.nih.gov/pubmed/35682819
http://dx.doi.org/10.3390/ijms23116136
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author Diallo, Aissatou Bailo
Mezouar, Soraya
Boumaza, Asma
Fiammingo, Oksana
Coiffard, Benjamin
Pontarotti, Pierre
Desnues, Benoit
Mege, Jean-Louis
author_facet Diallo, Aissatou Bailo
Mezouar, Soraya
Boumaza, Asma
Fiammingo, Oksana
Coiffard, Benjamin
Pontarotti, Pierre
Desnues, Benoit
Mege, Jean-Louis
author_sort Diallo, Aissatou Bailo
collection PubMed
description Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformatic approach was used to identify a homologue of KaiC (circadian gene in cyanobacteria) in Escherichia coli. Then, strains of E. coli (wild type and mutants) were grown on blood agar, and sampling was made every 3 h for 24 h at constant conditions. Gene expression was determined by qRT-PCR, and the rhythmicity was analyzed using the Cosinor model. We identified RadA as a KaiC homologue in E. coli. Expression of radA showed a circadian rhythm persisting at least 3 days, with a peak in the morning. The circadian expression of other E. coli genes was also observed. Gene circadian oscillations were lost in radA mutants of E. coli. This study provides evidence of molecular clock gene expression in E. coli with a circadian rhythm. Such a finding paves the way for new perspectives in antibacterial treatment.
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spelling pubmed-91813242022-06-10 RadA, a Key Gene of the Circadian Rhythm of Escherichia coli Diallo, Aissatou Bailo Mezouar, Soraya Boumaza, Asma Fiammingo, Oksana Coiffard, Benjamin Pontarotti, Pierre Desnues, Benoit Mege, Jean-Louis Int J Mol Sci Communication Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformatic approach was used to identify a homologue of KaiC (circadian gene in cyanobacteria) in Escherichia coli. Then, strains of E. coli (wild type and mutants) were grown on blood agar, and sampling was made every 3 h for 24 h at constant conditions. Gene expression was determined by qRT-PCR, and the rhythmicity was analyzed using the Cosinor model. We identified RadA as a KaiC homologue in E. coli. Expression of radA showed a circadian rhythm persisting at least 3 days, with a peak in the morning. The circadian expression of other E. coli genes was also observed. Gene circadian oscillations were lost in radA mutants of E. coli. This study provides evidence of molecular clock gene expression in E. coli with a circadian rhythm. Such a finding paves the way for new perspectives in antibacterial treatment. MDPI 2022-05-30 /pmc/articles/PMC9181324/ /pubmed/35682819 http://dx.doi.org/10.3390/ijms23116136 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Diallo, Aissatou Bailo
Mezouar, Soraya
Boumaza, Asma
Fiammingo, Oksana
Coiffard, Benjamin
Pontarotti, Pierre
Desnues, Benoit
Mege, Jean-Louis
RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title_full RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title_fullStr RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title_full_unstemmed RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title_short RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
title_sort rada, a key gene of the circadian rhythm of escherichia coli
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181324/
https://www.ncbi.nlm.nih.gov/pubmed/35682819
http://dx.doi.org/10.3390/ijms23116136
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