Cargando…
RadA, a Key Gene of the Circadian Rhythm of Escherichia coli
Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181324/ https://www.ncbi.nlm.nih.gov/pubmed/35682819 http://dx.doi.org/10.3390/ijms23116136 |
_version_ | 1784723742295851008 |
---|---|
author | Diallo, Aissatou Bailo Mezouar, Soraya Boumaza, Asma Fiammingo, Oksana Coiffard, Benjamin Pontarotti, Pierre Desnues, Benoit Mege, Jean-Louis |
author_facet | Diallo, Aissatou Bailo Mezouar, Soraya Boumaza, Asma Fiammingo, Oksana Coiffard, Benjamin Pontarotti, Pierre Desnues, Benoit Mege, Jean-Louis |
author_sort | Diallo, Aissatou Bailo |
collection | PubMed |
description | Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformatic approach was used to identify a homologue of KaiC (circadian gene in cyanobacteria) in Escherichia coli. Then, strains of E. coli (wild type and mutants) were grown on blood agar, and sampling was made every 3 h for 24 h at constant conditions. Gene expression was determined by qRT-PCR, and the rhythmicity was analyzed using the Cosinor model. We identified RadA as a KaiC homologue in E. coli. Expression of radA showed a circadian rhythm persisting at least 3 days, with a peak in the morning. The circadian expression of other E. coli genes was also observed. Gene circadian oscillations were lost in radA mutants of E. coli. This study provides evidence of molecular clock gene expression in E. coli with a circadian rhythm. Such a finding paves the way for new perspectives in antibacterial treatment. |
format | Online Article Text |
id | pubmed-9181324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91813242022-06-10 RadA, a Key Gene of the Circadian Rhythm of Escherichia coli Diallo, Aissatou Bailo Mezouar, Soraya Boumaza, Asma Fiammingo, Oksana Coiffard, Benjamin Pontarotti, Pierre Desnues, Benoit Mege, Jean-Louis Int J Mol Sci Communication Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformatic approach was used to identify a homologue of KaiC (circadian gene in cyanobacteria) in Escherichia coli. Then, strains of E. coli (wild type and mutants) were grown on blood agar, and sampling was made every 3 h for 24 h at constant conditions. Gene expression was determined by qRT-PCR, and the rhythmicity was analyzed using the Cosinor model. We identified RadA as a KaiC homologue in E. coli. Expression of radA showed a circadian rhythm persisting at least 3 days, with a peak in the morning. The circadian expression of other E. coli genes was also observed. Gene circadian oscillations were lost in radA mutants of E. coli. This study provides evidence of molecular clock gene expression in E. coli with a circadian rhythm. Such a finding paves the way for new perspectives in antibacterial treatment. MDPI 2022-05-30 /pmc/articles/PMC9181324/ /pubmed/35682819 http://dx.doi.org/10.3390/ijms23116136 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Diallo, Aissatou Bailo Mezouar, Soraya Boumaza, Asma Fiammingo, Oksana Coiffard, Benjamin Pontarotti, Pierre Desnues, Benoit Mege, Jean-Louis RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title | RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title_full | RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title_fullStr | RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title_full_unstemmed | RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title_short | RadA, a Key Gene of the Circadian Rhythm of Escherichia coli |
title_sort | rada, a key gene of the circadian rhythm of escherichia coli |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181324/ https://www.ncbi.nlm.nih.gov/pubmed/35682819 http://dx.doi.org/10.3390/ijms23116136 |
work_keys_str_mv | AT dialloaissatoubailo radaakeygeneofthecircadianrhythmofescherichiacoli AT mezouarsoraya radaakeygeneofthecircadianrhythmofescherichiacoli AT boumazaasma radaakeygeneofthecircadianrhythmofescherichiacoli AT fiammingooksana radaakeygeneofthecircadianrhythmofescherichiacoli AT coiffardbenjamin radaakeygeneofthecircadianrhythmofescherichiacoli AT pontarottipierre radaakeygeneofthecircadianrhythmofescherichiacoli AT desnuesbenoit radaakeygeneofthecircadianrhythmofescherichiacoli AT megejeanlouis radaakeygeneofthecircadianrhythmofescherichiacoli |