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Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity

Estrogen affects adipose tissue function. Therefore, this study aimed at assessing changes in the transcriptional activity of estrogen receptor (ER) α and β genes (ESR1 and ESR2, respectively) in the adipose tissues of obese individuals before and after weight loss and verifying whether epigenetic m...

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Autores principales: Koźniewski, Krzysztof, Wąsowski, Michał, Jonas, Marta Izabela, Lisik, Wojciech, Jonas, Maurycy, Binda, Artur, Jaworski, Paweł, Tarnowski, Wiesław, Noszczyk, Bartłomiej, Puzianowska-Kuźnicka, Monika, Kuryłowicz, Alina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181405/
https://www.ncbi.nlm.nih.gov/pubmed/35682668
http://dx.doi.org/10.3390/ijms23115989
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author Koźniewski, Krzysztof
Wąsowski, Michał
Jonas, Marta Izabela
Lisik, Wojciech
Jonas, Maurycy
Binda, Artur
Jaworski, Paweł
Tarnowski, Wiesław
Noszczyk, Bartłomiej
Puzianowska-Kuźnicka, Monika
Kuryłowicz, Alina
author_facet Koźniewski, Krzysztof
Wąsowski, Michał
Jonas, Marta Izabela
Lisik, Wojciech
Jonas, Maurycy
Binda, Artur
Jaworski, Paweł
Tarnowski, Wiesław
Noszczyk, Bartłomiej
Puzianowska-Kuźnicka, Monika
Kuryłowicz, Alina
author_sort Koźniewski, Krzysztof
collection PubMed
description Estrogen affects adipose tissue function. Therefore, this study aimed at assessing changes in the transcriptional activity of estrogen receptor (ER) α and β genes (ESR1 and ESR2, respectively) in the adipose tissues of obese individuals before and after weight loss and verifying whether epigenetic mechanisms were involved in this phenomenon. ESR1 and ESR2 mRNA and miRNA levels were evaluated using real-time PCR in visceral (VAT) and subcutaneous adipose tissue (SAT) of 78 obese (BMI > 40 kg/m(2)) and 31 normal-weight (BMI = 20–24.9 kg/m(2)) individuals and in 19 SAT samples from post-bariatric patients. ESR1 and ESR2 methylation status was studied using the methylation-sensitive digestion/real-time PCR method. Obesity was associated with a decrease in mRNA levels of both ERs in SAT (p < 0.0001) and ESR2 in VAT (p = 0.0001), while weight loss increased ESR transcription (p < 0.0001). Methylation levels of ESR1 and ESR2 promoters were unaffected. However, ESR1 mRNA in the AT of obese subjects correlated negatively with the expression of hsa-miR-18a-5p (r(s) = −0.444), hsa-miR-18b-5p (r(s) = −0.329), hsa-miR-22-3p (r(s) = −0.413), hsa-miR-100-5p (r(s) = −0.371), and hsa-miR-143-5p (r(s) = −0.289), while the expression of ESR2 in VAT correlated negatively with hsa-miR-576-5p (r(s) = −0.353) and in SAT with hsa-miR-495-3p (r(s) = −0.308). In conclusion, obesity-associated downregulation of ER mRNA levels in adipose tissue may result from miRNA interference.
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spelling pubmed-91814052022-06-10 Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity Koźniewski, Krzysztof Wąsowski, Michał Jonas, Marta Izabela Lisik, Wojciech Jonas, Maurycy Binda, Artur Jaworski, Paweł Tarnowski, Wiesław Noszczyk, Bartłomiej Puzianowska-Kuźnicka, Monika Kuryłowicz, Alina Int J Mol Sci Article Estrogen affects adipose tissue function. Therefore, this study aimed at assessing changes in the transcriptional activity of estrogen receptor (ER) α and β genes (ESR1 and ESR2, respectively) in the adipose tissues of obese individuals before and after weight loss and verifying whether epigenetic mechanisms were involved in this phenomenon. ESR1 and ESR2 mRNA and miRNA levels were evaluated using real-time PCR in visceral (VAT) and subcutaneous adipose tissue (SAT) of 78 obese (BMI > 40 kg/m(2)) and 31 normal-weight (BMI = 20–24.9 kg/m(2)) individuals and in 19 SAT samples from post-bariatric patients. ESR1 and ESR2 methylation status was studied using the methylation-sensitive digestion/real-time PCR method. Obesity was associated with a decrease in mRNA levels of both ERs in SAT (p < 0.0001) and ESR2 in VAT (p = 0.0001), while weight loss increased ESR transcription (p < 0.0001). Methylation levels of ESR1 and ESR2 promoters were unaffected. However, ESR1 mRNA in the AT of obese subjects correlated negatively with the expression of hsa-miR-18a-5p (r(s) = −0.444), hsa-miR-18b-5p (r(s) = −0.329), hsa-miR-22-3p (r(s) = −0.413), hsa-miR-100-5p (r(s) = −0.371), and hsa-miR-143-5p (r(s) = −0.289), while the expression of ESR2 in VAT correlated negatively with hsa-miR-576-5p (r(s) = −0.353) and in SAT with hsa-miR-495-3p (r(s) = −0.308). In conclusion, obesity-associated downregulation of ER mRNA levels in adipose tissue may result from miRNA interference. MDPI 2022-05-26 /pmc/articles/PMC9181405/ /pubmed/35682668 http://dx.doi.org/10.3390/ijms23115989 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koźniewski, Krzysztof
Wąsowski, Michał
Jonas, Marta Izabela
Lisik, Wojciech
Jonas, Maurycy
Binda, Artur
Jaworski, Paweł
Tarnowski, Wiesław
Noszczyk, Bartłomiej
Puzianowska-Kuźnicka, Monika
Kuryłowicz, Alina
Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title_full Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title_fullStr Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title_full_unstemmed Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title_short Epigenetic Regulation of Estrogen Receptor Genes’ Expressions in Adipose Tissue in the Course of Obesity
title_sort epigenetic regulation of estrogen receptor genes’ expressions in adipose tissue in the course of obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181405/
https://www.ncbi.nlm.nih.gov/pubmed/35682668
http://dx.doi.org/10.3390/ijms23115989
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