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A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response

Botryosphaeria spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were det...

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Autores principales: Xiao, Feng, Xu, Wenxing, Hong, Ni, Wang, Liping, Zhang, Yongle, Wang, Guoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181491/
https://www.ncbi.nlm.nih.gov/pubmed/35682745
http://dx.doi.org/10.3390/ijms23116066
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author Xiao, Feng
Xu, Wenxing
Hong, Ni
Wang, Liping
Zhang, Yongle
Wang, Guoping
author_facet Xiao, Feng
Xu, Wenxing
Hong, Ni
Wang, Liping
Zhang, Yongle
Wang, Guoping
author_sort Xiao, Feng
collection PubMed
description Botryosphaeria spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were detected in Botryosphaeria kuwatsukai, named BkLiP1 to BkLiP7, respectively, while only BkLiP1 was identified as responsible for the vegetative growth and virulence of B. kuwatsukai as assessed in combination with knock-out, complementation, and overexpression approaches. Moreover, BkLiP1, with the aid of a signal peptide (SP), is translocated onto the cell wall of B. kuwatsukai and secreted into the apoplast space of plant cells as expressed in the leaves of Nicotiana benthamiana, which can behave as a microbe-associated molecular pattern (MAMP) to trigger the defense response of plants, including cell death, reactive oxygen species (ROS) burst, callose deposition, and immunity-related genes up-regulated. It supports the conclusion that BkLiP1 plays an important role in the virulence and vegetative growth of B. kuwatsukai and alternatively behaves as an MAMP to induce plant cell death used for the fungal version, which contributes to a better understanding of the pathogenetic mechanism of Botryosphaeria fungi.
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spelling pubmed-91814912022-06-10 A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response Xiao, Feng Xu, Wenxing Hong, Ni Wang, Liping Zhang, Yongle Wang, Guoping Int J Mol Sci Article Botryosphaeria spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were detected in Botryosphaeria kuwatsukai, named BkLiP1 to BkLiP7, respectively, while only BkLiP1 was identified as responsible for the vegetative growth and virulence of B. kuwatsukai as assessed in combination with knock-out, complementation, and overexpression approaches. Moreover, BkLiP1, with the aid of a signal peptide (SP), is translocated onto the cell wall of B. kuwatsukai and secreted into the apoplast space of plant cells as expressed in the leaves of Nicotiana benthamiana, which can behave as a microbe-associated molecular pattern (MAMP) to trigger the defense response of plants, including cell death, reactive oxygen species (ROS) burst, callose deposition, and immunity-related genes up-regulated. It supports the conclusion that BkLiP1 plays an important role in the virulence and vegetative growth of B. kuwatsukai and alternatively behaves as an MAMP to induce plant cell death used for the fungal version, which contributes to a better understanding of the pathogenetic mechanism of Botryosphaeria fungi. MDPI 2022-05-28 /pmc/articles/PMC9181491/ /pubmed/35682745 http://dx.doi.org/10.3390/ijms23116066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Feng
Xu, Wenxing
Hong, Ni
Wang, Liping
Zhang, Yongle
Wang, Guoping
A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title_full A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title_fullStr A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title_full_unstemmed A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title_short A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response
title_sort secreted lignin peroxidase required for fungal growth and virulence and related to plant immune response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181491/
https://www.ncbi.nlm.nih.gov/pubmed/35682745
http://dx.doi.org/10.3390/ijms23116066
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