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Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer

Prostate cancer (PC) is a male common neoplasm and is the second leading cause of cancer death in American men. PC is traditionally diagnosed by the evaluation of prostate secreted antigen (PSA) in the blood. Due to the high levels of false positives, digital rectal examination and transrectal ultra...

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Autores principales: Bertoli, Gloria, Panio, Antonella, Cava, Claudia, Gallivanone, Francesca, Alini, Martina, Strano, Giulia, Molfino, Federico, Brioschi, Loredana, Viani, Paola, Porro, Danilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181550/
https://www.ncbi.nlm.nih.gov/pubmed/35683018
http://dx.doi.org/10.3390/ijms23116339
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author Bertoli, Gloria
Panio, Antonella
Cava, Claudia
Gallivanone, Francesca
Alini, Martina
Strano, Giulia
Molfino, Federico
Brioschi, Loredana
Viani, Paola
Porro, Danilo
author_facet Bertoli, Gloria
Panio, Antonella
Cava, Claudia
Gallivanone, Francesca
Alini, Martina
Strano, Giulia
Molfino, Federico
Brioschi, Loredana
Viani, Paola
Porro, Danilo
author_sort Bertoli, Gloria
collection PubMed
description Prostate cancer (PC) is a male common neoplasm and is the second leading cause of cancer death in American men. PC is traditionally diagnosed by the evaluation of prostate secreted antigen (PSA) in the blood. Due to the high levels of false positives, digital rectal examination and transrectal ultrasound guided biopsy are necessary in uncertain cases with elevated PSA levels. Nevertheless, the high mortality rate suggests that new PC biomarkers are urgently needed to help clinical diagnosis. In a previous study, we have identified a network of genes, altered in high Gleason Score (GS) PC (GS ≥ 7), being regulated by miR-153. Until now, no publication has explained the mechanism of action of miR-153 in PC. By in vitro studies, we found that the overexpression of miR-153 in high GS cell lines is required to control cell proliferation, migration and invasion rates, targeting Kruppel-like factor 5 (KLF5). Moreover, miR-153 could be secreted by exosomes and microvesicles in the microenvironment and, once entered into the surrounding tissue, could influence cellular growth. Being upregulated in high GS human PC, miR-153 could be proposed as a circulating biomarker for PC diagnosis.
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spelling pubmed-91815502022-06-10 Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer Bertoli, Gloria Panio, Antonella Cava, Claudia Gallivanone, Francesca Alini, Martina Strano, Giulia Molfino, Federico Brioschi, Loredana Viani, Paola Porro, Danilo Int J Mol Sci Article Prostate cancer (PC) is a male common neoplasm and is the second leading cause of cancer death in American men. PC is traditionally diagnosed by the evaluation of prostate secreted antigen (PSA) in the blood. Due to the high levels of false positives, digital rectal examination and transrectal ultrasound guided biopsy are necessary in uncertain cases with elevated PSA levels. Nevertheless, the high mortality rate suggests that new PC biomarkers are urgently needed to help clinical diagnosis. In a previous study, we have identified a network of genes, altered in high Gleason Score (GS) PC (GS ≥ 7), being regulated by miR-153. Until now, no publication has explained the mechanism of action of miR-153 in PC. By in vitro studies, we found that the overexpression of miR-153 in high GS cell lines is required to control cell proliferation, migration and invasion rates, targeting Kruppel-like factor 5 (KLF5). Moreover, miR-153 could be secreted by exosomes and microvesicles in the microenvironment and, once entered into the surrounding tissue, could influence cellular growth. Being upregulated in high GS human PC, miR-153 could be proposed as a circulating biomarker for PC diagnosis. MDPI 2022-06-06 /pmc/articles/PMC9181550/ /pubmed/35683018 http://dx.doi.org/10.3390/ijms23116339 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bertoli, Gloria
Panio, Antonella
Cava, Claudia
Gallivanone, Francesca
Alini, Martina
Strano, Giulia
Molfino, Federico
Brioschi, Loredana
Viani, Paola
Porro, Danilo
Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title_full Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title_fullStr Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title_full_unstemmed Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title_short Secreted miR-153 Controls Proliferation and Invasion of Higher Gleason Score Prostate Cancer
title_sort secreted mir-153 controls proliferation and invasion of higher gleason score prostate cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181550/
https://www.ncbi.nlm.nih.gov/pubmed/35683018
http://dx.doi.org/10.3390/ijms23116339
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