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Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo
Introduction: The effective induction of angiogenesis is directly related to the success of bone-substitute materials (BSM) for maxillofacial osseous regeneration. Therefore, the addition of pro-angiogenic properties to a commercially available bovine bone-substitute material in combination with hya...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181602/ https://www.ncbi.nlm.nih.gov/pubmed/35683136 http://dx.doi.org/10.3390/ma15113839 |
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author | Kyyak, Solomiya Blatt, Sebastian Wiesmann, Nadine Smeets, Ralf Kaemmerer, Peer W. |
author_facet | Kyyak, Solomiya Blatt, Sebastian Wiesmann, Nadine Smeets, Ralf Kaemmerer, Peer W. |
author_sort | Kyyak, Solomiya |
collection | PubMed |
description | Introduction: The effective induction of angiogenesis is directly related to the success of bone-substitute materials (BSM) for maxillofacial osseous regeneration. Therefore, the addition of pro-angiogenic properties to a commercially available bovine bone-substitute material in combination with hyaluronic acid (BSM+) was compared to the same bone-substitute material without hyaluronic acid (BSM) in an in-vivo model. Materials and Methods: BSM+ and BSM were incubated for six days on the chorioallantoic membrane (CAM) of fertilized chicken eggs. Microscopically, the number of vessels and branching points, the vessel area and vessel length were evaluated. Subsequently, the total vessel area and brightness integration were assessed after immunohistochemical staining (H&E, alphaSMA). Results: In the BSM+ group, a significantly higher number of vessels (p < 0.001), branching points (p = 0.001), total vessel area (p < 0.001) as well as vessel length (p = 0.001) were found in comparison to the BSM group without hyaluronic acid. Immunohistochemically, a significantly increased total vessel area (p < 0.001 for H&E, p = 0.037 for alphaSMA) and brightness integration (p = 0.047) for BSM+ in comparison to the native material were seen. Conclusions: The combination of a xenogenic bone-substitute material with hyaluronic acid significantly induced angiogenesis in vivo. This might lead to a faster integration and an improved healing in clinical situations. |
format | Online Article Text |
id | pubmed-9181602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91816022022-06-10 Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo Kyyak, Solomiya Blatt, Sebastian Wiesmann, Nadine Smeets, Ralf Kaemmerer, Peer W. Materials (Basel) Article Introduction: The effective induction of angiogenesis is directly related to the success of bone-substitute materials (BSM) for maxillofacial osseous regeneration. Therefore, the addition of pro-angiogenic properties to a commercially available bovine bone-substitute material in combination with hyaluronic acid (BSM+) was compared to the same bone-substitute material without hyaluronic acid (BSM) in an in-vivo model. Materials and Methods: BSM+ and BSM were incubated for six days on the chorioallantoic membrane (CAM) of fertilized chicken eggs. Microscopically, the number of vessels and branching points, the vessel area and vessel length were evaluated. Subsequently, the total vessel area and brightness integration were assessed after immunohistochemical staining (H&E, alphaSMA). Results: In the BSM+ group, a significantly higher number of vessels (p < 0.001), branching points (p = 0.001), total vessel area (p < 0.001) as well as vessel length (p = 0.001) were found in comparison to the BSM group without hyaluronic acid. Immunohistochemically, a significantly increased total vessel area (p < 0.001 for H&E, p = 0.037 for alphaSMA) and brightness integration (p = 0.047) for BSM+ in comparison to the native material were seen. Conclusions: The combination of a xenogenic bone-substitute material with hyaluronic acid significantly induced angiogenesis in vivo. This might lead to a faster integration and an improved healing in clinical situations. MDPI 2022-05-27 /pmc/articles/PMC9181602/ /pubmed/35683136 http://dx.doi.org/10.3390/ma15113839 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kyyak, Solomiya Blatt, Sebastian Wiesmann, Nadine Smeets, Ralf Kaemmerer, Peer W. Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title | Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title_full | Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title_fullStr | Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title_full_unstemmed | Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title_short | Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo |
title_sort | hyaluronic acid with bone substitutes enhance angiogenesis in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181602/ https://www.ncbi.nlm.nih.gov/pubmed/35683136 http://dx.doi.org/10.3390/ma15113839 |
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