Cargando…
Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing
(1) Background: Nanocomposite films are widely applied in the pharmaceutical industry (e.g., nanodrug delivery systems—NDDS). Indeed, these nanomaterials can be produced at a large industrial scale and display valuable properties (e.g., antibacterial, renewability, biodegradability, bioavailability,...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182010/ https://www.ncbi.nlm.nih.gov/pubmed/35683183 http://dx.doi.org/10.3390/ma15113885 |
_version_ | 1784723928929796096 |
---|---|
author | Bibi, Shabana Mir, Sadullah Rehman, Wajid Menaa, Farid Gul, Alia Alaryani, Fatima Saad Salem Alqahtani, Ali M. Haq, Sirajul Abdellatif, Magda H. |
author_facet | Bibi, Shabana Mir, Sadullah Rehman, Wajid Menaa, Farid Gul, Alia Alaryani, Fatima Saad Salem Alqahtani, Ali M. Haq, Sirajul Abdellatif, Magda H. |
author_sort | Bibi, Shabana |
collection | PubMed |
description | (1) Background: Nanocomposite films are widely applied in the pharmaceutical industry (e.g., nanodrug delivery systems—NDDS). Indeed, these nanomaterials can be produced at a large industrial scale and display valuable properties (e.g., antibacterial, renewability, biodegradability, bioavailability, safety, tissue-specific targeting, and biocompatibility), which can enhance the activity of conventional marketed drugs. (2) Aim: To fabricate and investigate the in vitro properties of the antibiotic ceftriaxone sodium (CTX) once encapsulated into sodium alginate (SA)/poly(vinyl alcohol)PVA-clay reinforced nanocomposite films. (3) Methods: Different ratios of the polymers (i.e., SA, PVA) and CTX drug were used for the synthesis of nanocomposite films by solvent casting technique. Montmorillonite (MMT), modified organically, was added as a nanofiller to increase their thermal and mechanical strength. The prepared samples were physically characterized by thermogravimetric analysis (TGA), X-ray diffraction (XRD), scanning electronic microscopy (SEM), and energy-dispersive X-ray analysis (EDX). The physicochemical behavior (i.e., swelling, erosion, dissolution/drug release behavior and rat skin permeation) was also assessed. Comparisons were made with the currently marketed free CTX dosage form. (4) Results: TGA of the nanoformulation showed increased thermostability. XRD revealed its semi-crystalline nature. SEM depicted a homogeneous drug-loaded SA/PVA nanocomposite with an average size ranging between 300 and 500 nm. EDX confirmed the elemental composition and uniform distribution of mixing components. The water entrapment efficiency study showed that the highest swelling and erosion ratio is encountered with the nanoformulations S100(3) and S100D15(3). Ex vivo permeation revealed a bi-step discharge mode with an early burst liberation chased by continued drug discharge of devised nanoparticles (NPs). The dissolution studies of the drug-loaded polymer nanocomposites elicited sustained pH-dependent drug release. The cumulative drug release was the highest (90.93%) with S100D15(3). (5) Conclusion: S100D15(3) was the finest formulation. To the best of our knowledge, we also pioneered the use of solvent casting for the preparation of such nanoformulations. Polymers and reinforcing agent, concentrations and pH were rate-deterring features for the preparation of the optimized formulation. Thus, CTX-loaded SA/PVA-MMT reinforced nanocomposite appeared as a promising nanodrug delivery system (NDDS) based on its in vitro physicochemical properties. |
format | Online Article Text |
id | pubmed-9182010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91820102022-06-10 Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing Bibi, Shabana Mir, Sadullah Rehman, Wajid Menaa, Farid Gul, Alia Alaryani, Fatima Saad Salem Alqahtani, Ali M. Haq, Sirajul Abdellatif, Magda H. Materials (Basel) Article (1) Background: Nanocomposite films are widely applied in the pharmaceutical industry (e.g., nanodrug delivery systems—NDDS). Indeed, these nanomaterials can be produced at a large industrial scale and display valuable properties (e.g., antibacterial, renewability, biodegradability, bioavailability, safety, tissue-specific targeting, and biocompatibility), which can enhance the activity of conventional marketed drugs. (2) Aim: To fabricate and investigate the in vitro properties of the antibiotic ceftriaxone sodium (CTX) once encapsulated into sodium alginate (SA)/poly(vinyl alcohol)PVA-clay reinforced nanocomposite films. (3) Methods: Different ratios of the polymers (i.e., SA, PVA) and CTX drug were used for the synthesis of nanocomposite films by solvent casting technique. Montmorillonite (MMT), modified organically, was added as a nanofiller to increase their thermal and mechanical strength. The prepared samples were physically characterized by thermogravimetric analysis (TGA), X-ray diffraction (XRD), scanning electronic microscopy (SEM), and energy-dispersive X-ray analysis (EDX). The physicochemical behavior (i.e., swelling, erosion, dissolution/drug release behavior and rat skin permeation) was also assessed. Comparisons were made with the currently marketed free CTX dosage form. (4) Results: TGA of the nanoformulation showed increased thermostability. XRD revealed its semi-crystalline nature. SEM depicted a homogeneous drug-loaded SA/PVA nanocomposite with an average size ranging between 300 and 500 nm. EDX confirmed the elemental composition and uniform distribution of mixing components. The water entrapment efficiency study showed that the highest swelling and erosion ratio is encountered with the nanoformulations S100(3) and S100D15(3). Ex vivo permeation revealed a bi-step discharge mode with an early burst liberation chased by continued drug discharge of devised nanoparticles (NPs). The dissolution studies of the drug-loaded polymer nanocomposites elicited sustained pH-dependent drug release. The cumulative drug release was the highest (90.93%) with S100D15(3). (5) Conclusion: S100D15(3) was the finest formulation. To the best of our knowledge, we also pioneered the use of solvent casting for the preparation of such nanoformulations. Polymers and reinforcing agent, concentrations and pH were rate-deterring features for the preparation of the optimized formulation. Thus, CTX-loaded SA/PVA-MMT reinforced nanocomposite appeared as a promising nanodrug delivery system (NDDS) based on its in vitro physicochemical properties. MDPI 2022-05-30 /pmc/articles/PMC9182010/ /pubmed/35683183 http://dx.doi.org/10.3390/ma15113885 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bibi, Shabana Mir, Sadullah Rehman, Wajid Menaa, Farid Gul, Alia Alaryani, Fatima Saad Salem Alqahtani, Ali M. Haq, Sirajul Abdellatif, Magda H. Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title | Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title_full | Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title_fullStr | Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title_full_unstemmed | Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title_short | Synthesis and In Vitro/Ex Vivo Characterizations of Ceftriaxone-Loaded Sodium Alginate/poly(vinyl alcohol) Clay Reinforced Nanocomposites: Possible Applications in Wound Healing |
title_sort | synthesis and in vitro/ex vivo characterizations of ceftriaxone-loaded sodium alginate/poly(vinyl alcohol) clay reinforced nanocomposites: possible applications in wound healing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182010/ https://www.ncbi.nlm.nih.gov/pubmed/35683183 http://dx.doi.org/10.3390/ma15113885 |
work_keys_str_mv | AT bibishabana synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT mirsadullah synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT rehmanwajid synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT menaafarid synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT gulalia synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT alaryanifatimasaadsalem synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT alqahtanialim synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT haqsirajul synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing AT abdellatifmagdah synthesisandinvitroexvivocharacterizationsofceftriaxoneloadedsodiumalginatepolyvinylalcoholclayreinforcednanocompositespossibleapplicationsinwoundhealing |