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Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182187/ https://www.ncbi.nlm.nih.gov/pubmed/35684485 http://dx.doi.org/10.3390/molecules27113548 |
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author | Zhang, Zhanjiang Shen, Guang Yang, Yihua Li, Cui Chen, Xiaoying Yang, Xiaonan Guo, Xiaoyun Miao, Jianhua Li, Li Lei, Ming |
author_facet | Zhang, Zhanjiang Shen, Guang Yang, Yihua Li, Cui Chen, Xiaoying Yang, Xiaonan Guo, Xiaoyun Miao, Jianhua Li, Li Lei, Ming |
author_sort | Zhang, Zhanjiang |
collection | PubMed |
description | The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on only the rubber of TKS, without profiling the other metabolites in a systematic and comprehensive way. Here, the primary and secondary metabolites from the leaves of TKS were investigated using UPLC–ESI–MS/MS, and a total of 229 metabolites were characterized. Carboxylic acid derivatives, fatty acyls, phenols, and organooxygen compounds were found to be the major metabolites of TKS. The transcriptome data indicated that ribosomal, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, and linoleic acid metabolism genes were significantly differentially expressed. This study is the first to report the differences in the metabolic and transcriptome profiles of TKS leaves under exogenous ethephon spray, which improves our understanding of the main metabolites and their molecular mechanisms in TKS leaves. |
format | Online Article Text |
id | pubmed-9182187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91821872022-06-10 Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin Zhang, Zhanjiang Shen, Guang Yang, Yihua Li, Cui Chen, Xiaoying Yang, Xiaonan Guo, Xiaoyun Miao, Jianhua Li, Li Lei, Ming Molecules Article The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on only the rubber of TKS, without profiling the other metabolites in a systematic and comprehensive way. Here, the primary and secondary metabolites from the leaves of TKS were investigated using UPLC–ESI–MS/MS, and a total of 229 metabolites were characterized. Carboxylic acid derivatives, fatty acyls, phenols, and organooxygen compounds were found to be the major metabolites of TKS. The transcriptome data indicated that ribosomal, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, and linoleic acid metabolism genes were significantly differentially expressed. This study is the first to report the differences in the metabolic and transcriptome profiles of TKS leaves under exogenous ethephon spray, which improves our understanding of the main metabolites and their molecular mechanisms in TKS leaves. MDPI 2022-05-31 /pmc/articles/PMC9182187/ /pubmed/35684485 http://dx.doi.org/10.3390/molecules27113548 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Zhanjiang Shen, Guang Yang, Yihua Li, Cui Chen, Xiaoying Yang, Xiaonan Guo, Xiaoyun Miao, Jianhua Li, Li Lei, Ming Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title | Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title_full | Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title_fullStr | Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title_full_unstemmed | Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title_short | Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin |
title_sort | metabolic and transcriptomic analyses reveal the effects of ethephon on taraxacum kok-saghyz rodin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182187/ https://www.ncbi.nlm.nih.gov/pubmed/35684485 http://dx.doi.org/10.3390/molecules27113548 |
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