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Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin

The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on...

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Autores principales: Zhang, Zhanjiang, Shen, Guang, Yang, Yihua, Li, Cui, Chen, Xiaoying, Yang, Xiaonan, Guo, Xiaoyun, Miao, Jianhua, Li, Li, Lei, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182187/
https://www.ncbi.nlm.nih.gov/pubmed/35684485
http://dx.doi.org/10.3390/molecules27113548
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author Zhang, Zhanjiang
Shen, Guang
Yang, Yihua
Li, Cui
Chen, Xiaoying
Yang, Xiaonan
Guo, Xiaoyun
Miao, Jianhua
Li, Li
Lei, Ming
author_facet Zhang, Zhanjiang
Shen, Guang
Yang, Yihua
Li, Cui
Chen, Xiaoying
Yang, Xiaonan
Guo, Xiaoyun
Miao, Jianhua
Li, Li
Lei, Ming
author_sort Zhang, Zhanjiang
collection PubMed
description The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on only the rubber of TKS, without profiling the other metabolites in a systematic and comprehensive way. Here, the primary and secondary metabolites from the leaves of TKS were investigated using UPLC–ESI–MS/MS, and a total of 229 metabolites were characterized. Carboxylic acid derivatives, fatty acyls, phenols, and organooxygen compounds were found to be the major metabolites of TKS. The transcriptome data indicated that ribosomal, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, and linoleic acid metabolism genes were significantly differentially expressed. This study is the first to report the differences in the metabolic and transcriptome profiles of TKS leaves under exogenous ethephon spray, which improves our understanding of the main metabolites and their molecular mechanisms in TKS leaves.
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spelling pubmed-91821872022-06-10 Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin Zhang, Zhanjiang Shen, Guang Yang, Yihua Li, Cui Chen, Xiaoying Yang, Xiaonan Guo, Xiaoyun Miao, Jianhua Li, Li Lei, Ming Molecules Article The roots of Taraxacum kok-saghyz Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on only the rubber of TKS, without profiling the other metabolites in a systematic and comprehensive way. Here, the primary and secondary metabolites from the leaves of TKS were investigated using UPLC–ESI–MS/MS, and a total of 229 metabolites were characterized. Carboxylic acid derivatives, fatty acyls, phenols, and organooxygen compounds were found to be the major metabolites of TKS. The transcriptome data indicated that ribosomal, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, and linoleic acid metabolism genes were significantly differentially expressed. This study is the first to report the differences in the metabolic and transcriptome profiles of TKS leaves under exogenous ethephon spray, which improves our understanding of the main metabolites and their molecular mechanisms in TKS leaves. MDPI 2022-05-31 /pmc/articles/PMC9182187/ /pubmed/35684485 http://dx.doi.org/10.3390/molecules27113548 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Zhanjiang
Shen, Guang
Yang, Yihua
Li, Cui
Chen, Xiaoying
Yang, Xiaonan
Guo, Xiaoyun
Miao, Jianhua
Li, Li
Lei, Ming
Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title_full Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title_fullStr Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title_full_unstemmed Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title_short Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon on Taraxacum kok-saghyz Rodin
title_sort metabolic and transcriptomic analyses reveal the effects of ethephon on taraxacum kok-saghyz rodin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182187/
https://www.ncbi.nlm.nih.gov/pubmed/35684485
http://dx.doi.org/10.3390/molecules27113548
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