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Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study

Carotid atherosclerotic plaque rupture and its sequelae are among the leading causes of acute ischemic stroke. The risk of rupture and subsequent thrombosis is, among others, determined by vulnerable plaque characteristics and linked to activation of the immune system, in which neutrophil extracellu...

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Autores principales: de Vries, Judith J., Autar, Anouchska S. A., van Dam-Nolen, Dianne H. K., Donkel, Samantha J., Kassem, Mohamed, van der Kolk, Anja G., van Velzen, Twan J., Kooi, M. Eline, Hendrikse, Jeroen, Nederkoorn, Paul J., Bos, Daniel, van der Lugt, Aad, de Maat, Moniek P. M., van Beusekom, Heleen M. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182254/
https://www.ncbi.nlm.nih.gov/pubmed/35679310
http://dx.doi.org/10.1371/journal.pone.0269805
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author de Vries, Judith J.
Autar, Anouchska S. A.
van Dam-Nolen, Dianne H. K.
Donkel, Samantha J.
Kassem, Mohamed
van der Kolk, Anja G.
van Velzen, Twan J.
Kooi, M. Eline
Hendrikse, Jeroen
Nederkoorn, Paul J.
Bos, Daniel
van der Lugt, Aad
de Maat, Moniek P. M.
van Beusekom, Heleen M. M.
author_facet de Vries, Judith J.
Autar, Anouchska S. A.
van Dam-Nolen, Dianne H. K.
Donkel, Samantha J.
Kassem, Mohamed
van der Kolk, Anja G.
van Velzen, Twan J.
Kooi, M. Eline
Hendrikse, Jeroen
Nederkoorn, Paul J.
Bos, Daniel
van der Lugt, Aad
de Maat, Moniek P. M.
van Beusekom, Heleen M. M.
author_sort de Vries, Judith J.
collection PubMed
description Carotid atherosclerotic plaque rupture and its sequelae are among the leading causes of acute ischemic stroke. The risk of rupture and subsequent thrombosis is, among others, determined by vulnerable plaque characteristics and linked to activation of the immune system, in which neutrophil extracellular traps (NETs) potentially play a role. The aim of this study was to investigate how plaque vulnerability is associated with NETs levels. We included 182 patients from the Plaque At RISK (PARISK) study in whom carotid imaging was performed to measure plaque ulceration, fibrous cap integrity, intraplaque hemorrhage, lipid-rich necrotic core, calcifications and plaque volume. Principal component analysis generated a ‘vulnerability index’ comprising all plaque characteristics. Levels of the NETs marker myeloperoxidase-DNA complex were measured in patient plasma. The association between the vulnerability index and low or high NETs levels (dependent variable) was assessed by logistic regression. No significant association between the vulnerability index and NETs levels was detected in the total population (odds ratio 1.28, 95% confidence interval 0.90–1.83, p = 0.18). However, in the subgroup of patients naive to statins or antithrombotic medication prior to the index event, this association was statistically significant (odds ratio 2.08, 95% confidence interval 1.04–4.17, p = 0.04). Further analyses revealed that this positive association was mainly driven by intraplaque hemorrhage, lipid-rich necrotic core and ulceration. In conclusion, plaque vulnerability is positively associated with plasma levels of NETs, but only in patients naive to statins or antithrombotic medication prior to the index event.
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spelling pubmed-91822542022-06-10 Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study de Vries, Judith J. Autar, Anouchska S. A. van Dam-Nolen, Dianne H. K. Donkel, Samantha J. Kassem, Mohamed van der Kolk, Anja G. van Velzen, Twan J. Kooi, M. Eline Hendrikse, Jeroen Nederkoorn, Paul J. Bos, Daniel van der Lugt, Aad de Maat, Moniek P. M. van Beusekom, Heleen M. M. PLoS One Research Article Carotid atherosclerotic plaque rupture and its sequelae are among the leading causes of acute ischemic stroke. The risk of rupture and subsequent thrombosis is, among others, determined by vulnerable plaque characteristics and linked to activation of the immune system, in which neutrophil extracellular traps (NETs) potentially play a role. The aim of this study was to investigate how plaque vulnerability is associated with NETs levels. We included 182 patients from the Plaque At RISK (PARISK) study in whom carotid imaging was performed to measure plaque ulceration, fibrous cap integrity, intraplaque hemorrhage, lipid-rich necrotic core, calcifications and plaque volume. Principal component analysis generated a ‘vulnerability index’ comprising all plaque characteristics. Levels of the NETs marker myeloperoxidase-DNA complex were measured in patient plasma. The association between the vulnerability index and low or high NETs levels (dependent variable) was assessed by logistic regression. No significant association between the vulnerability index and NETs levels was detected in the total population (odds ratio 1.28, 95% confidence interval 0.90–1.83, p = 0.18). However, in the subgroup of patients naive to statins or antithrombotic medication prior to the index event, this association was statistically significant (odds ratio 2.08, 95% confidence interval 1.04–4.17, p = 0.04). Further analyses revealed that this positive association was mainly driven by intraplaque hemorrhage, lipid-rich necrotic core and ulceration. In conclusion, plaque vulnerability is positively associated with plasma levels of NETs, but only in patients naive to statins or antithrombotic medication prior to the index event. Public Library of Science 2022-06-09 /pmc/articles/PMC9182254/ /pubmed/35679310 http://dx.doi.org/10.1371/journal.pone.0269805 Text en © 2022 de Vries et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Vries, Judith J.
Autar, Anouchska S. A.
van Dam-Nolen, Dianne H. K.
Donkel, Samantha J.
Kassem, Mohamed
van der Kolk, Anja G.
van Velzen, Twan J.
Kooi, M. Eline
Hendrikse, Jeroen
Nederkoorn, Paul J.
Bos, Daniel
van der Lugt, Aad
de Maat, Moniek P. M.
van Beusekom, Heleen M. M.
Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title_full Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title_fullStr Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title_full_unstemmed Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title_short Association between plaque vulnerability and neutrophil extracellular traps (NETs) levels: The Plaque At RISK study
title_sort association between plaque vulnerability and neutrophil extracellular traps (nets) levels: the plaque at risk study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182254/
https://www.ncbi.nlm.nih.gov/pubmed/35679310
http://dx.doi.org/10.1371/journal.pone.0269805
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