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Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments

In this study, we aimed to isolate bacteria capable of degrading the polysaccharide ulvan from the green algae Ulva sp. (Chlorophyta, Ulvales, Ulvaceae) in marine environments. We isolated 13 ulvan-degrading bacteria and observed high diversity at the genus level. Further, the genera Paraglaciecola,...

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Autores principales: Tanaka, Reiji, Kurishiba, Yu, Miyake, Hideo, Shibata, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182395/
https://www.ncbi.nlm.nih.gov/pubmed/35684358
http://dx.doi.org/10.3390/molecules27113420
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author Tanaka, Reiji
Kurishiba, Yu
Miyake, Hideo
Shibata, Toshiyuki
author_facet Tanaka, Reiji
Kurishiba, Yu
Miyake, Hideo
Shibata, Toshiyuki
author_sort Tanaka, Reiji
collection PubMed
description In this study, we aimed to isolate bacteria capable of degrading the polysaccharide ulvan from the green algae Ulva sp. (Chlorophyta, Ulvales, Ulvaceae) in marine environments. We isolated 13 ulvan-degrading bacteria and observed high diversity at the genus level. Further, the genera Paraglaciecola, Vibrio, Echinicola, and Algibacter, which can degrade ulvan, were successfully isolated for the first time from marine environments. Among the 13 isolates, only one isolate (Echinicola sp.) showed the ability not only to produce externally expressed ulvan lyase, but also to be periplasmic or on the cell surface. From the results of the full-genome analysis, lyase was presumed to be a member of the PL25 (BNR4) family of ulvan lyases, and the bacterium also contained the sequence for glycoside hydrolase (GH43, GH78 and GH88), which is characteristic of other ulvan-degrading bacteria. Notably, this bacterium has a unique ulvan lyase gene not previously reported.
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spelling pubmed-91823952022-06-10 Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments Tanaka, Reiji Kurishiba, Yu Miyake, Hideo Shibata, Toshiyuki Molecules Article In this study, we aimed to isolate bacteria capable of degrading the polysaccharide ulvan from the green algae Ulva sp. (Chlorophyta, Ulvales, Ulvaceae) in marine environments. We isolated 13 ulvan-degrading bacteria and observed high diversity at the genus level. Further, the genera Paraglaciecola, Vibrio, Echinicola, and Algibacter, which can degrade ulvan, were successfully isolated for the first time from marine environments. Among the 13 isolates, only one isolate (Echinicola sp.) showed the ability not only to produce externally expressed ulvan lyase, but also to be periplasmic or on the cell surface. From the results of the full-genome analysis, lyase was presumed to be a member of the PL25 (BNR4) family of ulvan lyases, and the bacterium also contained the sequence for glycoside hydrolase (GH43, GH78 and GH88), which is characteristic of other ulvan-degrading bacteria. Notably, this bacterium has a unique ulvan lyase gene not previously reported. MDPI 2022-05-25 /pmc/articles/PMC9182395/ /pubmed/35684358 http://dx.doi.org/10.3390/molecules27113420 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanaka, Reiji
Kurishiba, Yu
Miyake, Hideo
Shibata, Toshiyuki
Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title_full Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title_fullStr Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title_full_unstemmed Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title_short Isolation, Diversity and Characterization of Ulvan-Degrading Bacteria Isolated from Marine Environments
title_sort isolation, diversity and characterization of ulvan-degrading bacteria isolated from marine environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182395/
https://www.ncbi.nlm.nih.gov/pubmed/35684358
http://dx.doi.org/10.3390/molecules27113420
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