Cargando…
nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis
Bovine mastitis is one of the most common diseases in dairy cows, and it causes significant economic losses in dairy industries worldwide. Gram-positive and Gram-negative bacteria can cause bovine mastitis, and many of them have developed antimicrobial resistance. There is an urgent need for novel t...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182459/ https://www.ncbi.nlm.nih.gov/pubmed/35684556 http://dx.doi.org/10.3390/molecules27113620 |
_version_ | 1784724040764620800 |
---|---|
author | Morales-Ubaldo, Ana Lizet Gonzalez-Cortazar, Manases Zaragoza-Bastida, Adrian Meza-Nieto, Martín A. Valladares-Carranza, Benjamín A. Alsayegh, Abdulrahman El-Saber Batiha, Gaber Rivero-Perez, Nallely |
author_facet | Morales-Ubaldo, Ana Lizet Gonzalez-Cortazar, Manases Zaragoza-Bastida, Adrian Meza-Nieto, Martín A. Valladares-Carranza, Benjamín A. Alsayegh, Abdulrahman El-Saber Batiha, Gaber Rivero-Perez, Nallely |
author_sort | Morales-Ubaldo, Ana Lizet |
collection | PubMed |
description | Bovine mastitis is one of the most common diseases in dairy cows, and it causes significant economic losses in dairy industries worldwide. Gram-positive and Gram-negative bacteria can cause bovine mastitis, and many of them have developed antimicrobial resistance. There is an urgent need for novel therapeutic options to treat the disease. Larrea tridentata-derived compounds represent an important potential alternative treatment. The aim of the present study was to isolate and characterize antibacterial compounds from Larrea tridentata against multidrug-resistant bacteria associated with bovine mastitis. The L. tridentata hydroalcoholic extract (LTHE) exhibited antibacterial activity. The extract was subjected to a bipartition, giving an aqueous fraction (moderate antibacterial activity) and an organic fraction (higher antibacterial activity). Chromatographic separation of the organic fraction enabled us to obtain four active sub-fractions. Chemical analyses through HPLC techniques were conducted for the LTHE, fractions, and sub-fraction Ltc1-F3, from which we isolated two compounds, characterized by (1)H and (13)C NMR analyses. Compound nor-3 demethoxyisoguaiacin exhibited the best antibacterial activity against the evaluated bacteria (MIC: 0.01–3.12 mg/mL; MBC: 0.02–3.12 mg/mL). The results indicated that nor-3 demethoxyisoguaiacin can be used as an alternative treatment for multidrug-resistant bacteria associated with mastitis. |
format | Online Article Text |
id | pubmed-9182459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91824592022-06-10 nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis Morales-Ubaldo, Ana Lizet Gonzalez-Cortazar, Manases Zaragoza-Bastida, Adrian Meza-Nieto, Martín A. Valladares-Carranza, Benjamín A. Alsayegh, Abdulrahman El-Saber Batiha, Gaber Rivero-Perez, Nallely Molecules Article Bovine mastitis is one of the most common diseases in dairy cows, and it causes significant economic losses in dairy industries worldwide. Gram-positive and Gram-negative bacteria can cause bovine mastitis, and many of them have developed antimicrobial resistance. There is an urgent need for novel therapeutic options to treat the disease. Larrea tridentata-derived compounds represent an important potential alternative treatment. The aim of the present study was to isolate and characterize antibacterial compounds from Larrea tridentata against multidrug-resistant bacteria associated with bovine mastitis. The L. tridentata hydroalcoholic extract (LTHE) exhibited antibacterial activity. The extract was subjected to a bipartition, giving an aqueous fraction (moderate antibacterial activity) and an organic fraction (higher antibacterial activity). Chromatographic separation of the organic fraction enabled us to obtain four active sub-fractions. Chemical analyses through HPLC techniques were conducted for the LTHE, fractions, and sub-fraction Ltc1-F3, from which we isolated two compounds, characterized by (1)H and (13)C NMR analyses. Compound nor-3 demethoxyisoguaiacin exhibited the best antibacterial activity against the evaluated bacteria (MIC: 0.01–3.12 mg/mL; MBC: 0.02–3.12 mg/mL). The results indicated that nor-3 demethoxyisoguaiacin can be used as an alternative treatment for multidrug-resistant bacteria associated with mastitis. MDPI 2022-06-05 /pmc/articles/PMC9182459/ /pubmed/35684556 http://dx.doi.org/10.3390/molecules27113620 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morales-Ubaldo, Ana Lizet Gonzalez-Cortazar, Manases Zaragoza-Bastida, Adrian Meza-Nieto, Martín A. Valladares-Carranza, Benjamín A. Alsayegh, Abdulrahman El-Saber Batiha, Gaber Rivero-Perez, Nallely nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title | nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title_full | nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title_fullStr | nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title_full_unstemmed | nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title_short | nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis |
title_sort | nor 3′-demethoxyisoguaiacin from larrea tridentata is a potential alternative against multidrug-resistant bacteria associated with bovine mastitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182459/ https://www.ncbi.nlm.nih.gov/pubmed/35684556 http://dx.doi.org/10.3390/molecules27113620 |
work_keys_str_mv | AT moralesubaldoanalizet nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT gonzalezcortazarmanases nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT zaragozabastidaadrian nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT mezanietomartina nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT valladarescarranzabenjamin nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT aalsayeghabdulrahman nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT elsaberbatihagaber nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis AT riveropereznallely nor3demethoxyisoguaiacinfromlarreatridentataisapotentialalternativeagainstmultidrugresistantbacteriaassociatedwithbovinemastitis |