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Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182470/ https://www.ncbi.nlm.nih.gov/pubmed/35683998 http://dx.doi.org/10.3390/nu14112198 |
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author | Naimimohasses, Sara O’Gorman, Philip Wright, Ciara Ni Fhloinn, Deirdre Holden, Dean Conlon, Niall Monaghan, Ann Kennedy, Megan Gormley, John Beddy, Peter Finn, Stephen Patrick Lysaght, Joanne O’Sullivan, Jacintha Dunne, Margaret R. Norris, Suzanne Moore, J. Bernadette |
author_facet | Naimimohasses, Sara O’Gorman, Philip Wright, Ciara Ni Fhloinn, Deirdre Holden, Dean Conlon, Niall Monaghan, Ann Kennedy, Megan Gormley, John Beddy, Peter Finn, Stephen Patrick Lysaght, Joanne O’Sullivan, Jacintha Dunne, Margaret R. Norris, Suzanne Moore, J. Bernadette |
author_sort | Naimimohasses, Sara |
collection | PubMed |
description | Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to examine circulating and intrahepatic MAIT cell populations in patients with NAFLD, after either 12 weeks of dietary intervention (DI) or aerobic exercise intervention (EI). Methods: Multicolour flow cytometry was used to immunophenotype circulating and intrahepatic MAIT cells and measure MAIT cell expression (median fluorescence intensity, MFI) of the activation marker CD69 and apoptotic marker CD95. Liver histology, clinical parameters, and MAIT cell populations were assessed at baseline (T0) and following completion (T1) of DI or EI. Results: Forty-five patients completed the study. DI participants showed decreased median (interquartile range) expression of the activation marker CD69 on circulating MAIT cells (T0: 104 (134) versus T1 27 (114) MFI; p = 0.0353) and improvements in histological steatosis grade post-intervention. EI participants showed increased expression of the apoptotic marker CD95, both in circulating (T0: 1549 (888) versus T1: 2563 (1371) MFI; p = 0.0043) and intrahepatic MAIT cells (T0: 2724 (862) versus T1: 3117 (1622) MFI; p = 0.0269). Moreover, the percentage of intrahepatic MAIT cells significantly decreased after EI (T0: 11.1 (14.4) versus T1: 5.3 (9.3)%; p = 0.0029), in conjunction with significant improvements in fibrosis stage and hepatocyte ballooning. Conclusions: These data demonstrate independent benefits from dietary and exercise intervention and suggest a role for intrahepatic MAIT cells in the observed histological improvements in NAFLD. |
format | Online Article Text |
id | pubmed-9182470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91824702022-06-10 Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD Naimimohasses, Sara O’Gorman, Philip Wright, Ciara Ni Fhloinn, Deirdre Holden, Dean Conlon, Niall Monaghan, Ann Kennedy, Megan Gormley, John Beddy, Peter Finn, Stephen Patrick Lysaght, Joanne O’Sullivan, Jacintha Dunne, Margaret R. Norris, Suzanne Moore, J. Bernadette Nutrients Article Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to examine circulating and intrahepatic MAIT cell populations in patients with NAFLD, after either 12 weeks of dietary intervention (DI) or aerobic exercise intervention (EI). Methods: Multicolour flow cytometry was used to immunophenotype circulating and intrahepatic MAIT cells and measure MAIT cell expression (median fluorescence intensity, MFI) of the activation marker CD69 and apoptotic marker CD95. Liver histology, clinical parameters, and MAIT cell populations were assessed at baseline (T0) and following completion (T1) of DI or EI. Results: Forty-five patients completed the study. DI participants showed decreased median (interquartile range) expression of the activation marker CD69 on circulating MAIT cells (T0: 104 (134) versus T1 27 (114) MFI; p = 0.0353) and improvements in histological steatosis grade post-intervention. EI participants showed increased expression of the apoptotic marker CD95, both in circulating (T0: 1549 (888) versus T1: 2563 (1371) MFI; p = 0.0043) and intrahepatic MAIT cells (T0: 2724 (862) versus T1: 3117 (1622) MFI; p = 0.0269). Moreover, the percentage of intrahepatic MAIT cells significantly decreased after EI (T0: 11.1 (14.4) versus T1: 5.3 (9.3)%; p = 0.0029), in conjunction with significant improvements in fibrosis stage and hepatocyte ballooning. Conclusions: These data demonstrate independent benefits from dietary and exercise intervention and suggest a role for intrahepatic MAIT cells in the observed histological improvements in NAFLD. MDPI 2022-05-25 /pmc/articles/PMC9182470/ /pubmed/35683998 http://dx.doi.org/10.3390/nu14112198 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Naimimohasses, Sara O’Gorman, Philip Wright, Ciara Ni Fhloinn, Deirdre Holden, Dean Conlon, Niall Monaghan, Ann Kennedy, Megan Gormley, John Beddy, Peter Finn, Stephen Patrick Lysaght, Joanne O’Sullivan, Jacintha Dunne, Margaret R. Norris, Suzanne Moore, J. Bernadette Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title | Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title_full | Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title_fullStr | Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title_full_unstemmed | Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title_short | Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD |
title_sort | differential effects of dietary versus exercise intervention on intrahepatic mait cells and histological features of nafld |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182470/ https://www.ncbi.nlm.nih.gov/pubmed/35683998 http://dx.doi.org/10.3390/nu14112198 |
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