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Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD

Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to...

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Autores principales: Naimimohasses, Sara, O’Gorman, Philip, Wright, Ciara, Ni Fhloinn, Deirdre, Holden, Dean, Conlon, Niall, Monaghan, Ann, Kennedy, Megan, Gormley, John, Beddy, Peter, Finn, Stephen Patrick, Lysaght, Joanne, O’Sullivan, Jacintha, Dunne, Margaret R., Norris, Suzanne, Moore, J. Bernadette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182470/
https://www.ncbi.nlm.nih.gov/pubmed/35683998
http://dx.doi.org/10.3390/nu14112198
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author Naimimohasses, Sara
O’Gorman, Philip
Wright, Ciara
Ni Fhloinn, Deirdre
Holden, Dean
Conlon, Niall
Monaghan, Ann
Kennedy, Megan
Gormley, John
Beddy, Peter
Finn, Stephen Patrick
Lysaght, Joanne
O’Sullivan, Jacintha
Dunne, Margaret R.
Norris, Suzanne
Moore, J. Bernadette
author_facet Naimimohasses, Sara
O’Gorman, Philip
Wright, Ciara
Ni Fhloinn, Deirdre
Holden, Dean
Conlon, Niall
Monaghan, Ann
Kennedy, Megan
Gormley, John
Beddy, Peter
Finn, Stephen Patrick
Lysaght, Joanne
O’Sullivan, Jacintha
Dunne, Margaret R.
Norris, Suzanne
Moore, J. Bernadette
author_sort Naimimohasses, Sara
collection PubMed
description Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to examine circulating and intrahepatic MAIT cell populations in patients with NAFLD, after either 12 weeks of dietary intervention (DI) or aerobic exercise intervention (EI). Methods: Multicolour flow cytometry was used to immunophenotype circulating and intrahepatic MAIT cells and measure MAIT cell expression (median fluorescence intensity, MFI) of the activation marker CD69 and apoptotic marker CD95. Liver histology, clinical parameters, and MAIT cell populations were assessed at baseline (T0) and following completion (T1) of DI or EI. Results: Forty-five patients completed the study. DI participants showed decreased median (interquartile range) expression of the activation marker CD69 on circulating MAIT cells (T0: 104 (134) versus T1 27 (114) MFI; p = 0.0353) and improvements in histological steatosis grade post-intervention. EI participants showed increased expression of the apoptotic marker CD95, both in circulating (T0: 1549 (888) versus T1: 2563 (1371) MFI; p = 0.0043) and intrahepatic MAIT cells (T0: 2724 (862) versus T1: 3117 (1622) MFI; p = 0.0269). Moreover, the percentage of intrahepatic MAIT cells significantly decreased after EI (T0: 11.1 (14.4) versus T1: 5.3 (9.3)%; p = 0.0029), in conjunction with significant improvements in fibrosis stage and hepatocyte ballooning. Conclusions: These data demonstrate independent benefits from dietary and exercise intervention and suggest a role for intrahepatic MAIT cells in the observed histological improvements in NAFLD.
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spelling pubmed-91824702022-06-10 Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD Naimimohasses, Sara O’Gorman, Philip Wright, Ciara Ni Fhloinn, Deirdre Holden, Dean Conlon, Niall Monaghan, Ann Kennedy, Megan Gormley, John Beddy, Peter Finn, Stephen Patrick Lysaght, Joanne O’Sullivan, Jacintha Dunne, Margaret R. Norris, Suzanne Moore, J. Bernadette Nutrients Article Background: Mucosal-associated invariant T (MAIT) cells promote inflammation in obesity and are implicated in the progression of non-alcoholic fatty liver disease (NAFLD). However, as the intrahepatic MAIT cell response to lifestyle intervention in NAFLD has not been investigated, this work aimed to examine circulating and intrahepatic MAIT cell populations in patients with NAFLD, after either 12 weeks of dietary intervention (DI) or aerobic exercise intervention (EI). Methods: Multicolour flow cytometry was used to immunophenotype circulating and intrahepatic MAIT cells and measure MAIT cell expression (median fluorescence intensity, MFI) of the activation marker CD69 and apoptotic marker CD95. Liver histology, clinical parameters, and MAIT cell populations were assessed at baseline (T0) and following completion (T1) of DI or EI. Results: Forty-five patients completed the study. DI participants showed decreased median (interquartile range) expression of the activation marker CD69 on circulating MAIT cells (T0: 104 (134) versus T1 27 (114) MFI; p = 0.0353) and improvements in histological steatosis grade post-intervention. EI participants showed increased expression of the apoptotic marker CD95, both in circulating (T0: 1549 (888) versus T1: 2563 (1371) MFI; p = 0.0043) and intrahepatic MAIT cells (T0: 2724 (862) versus T1: 3117 (1622) MFI; p = 0.0269). Moreover, the percentage of intrahepatic MAIT cells significantly decreased after EI (T0: 11.1 (14.4) versus T1: 5.3 (9.3)%; p = 0.0029), in conjunction with significant improvements in fibrosis stage and hepatocyte ballooning. Conclusions: These data demonstrate independent benefits from dietary and exercise intervention and suggest a role for intrahepatic MAIT cells in the observed histological improvements in NAFLD. MDPI 2022-05-25 /pmc/articles/PMC9182470/ /pubmed/35683998 http://dx.doi.org/10.3390/nu14112198 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naimimohasses, Sara
O’Gorman, Philip
Wright, Ciara
Ni Fhloinn, Deirdre
Holden, Dean
Conlon, Niall
Monaghan, Ann
Kennedy, Megan
Gormley, John
Beddy, Peter
Finn, Stephen Patrick
Lysaght, Joanne
O’Sullivan, Jacintha
Dunne, Margaret R.
Norris, Suzanne
Moore, J. Bernadette
Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title_full Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title_fullStr Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title_full_unstemmed Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title_short Differential Effects of Dietary versus Exercise Intervention on Intrahepatic MAIT Cells and Histological Features of NAFLD
title_sort differential effects of dietary versus exercise intervention on intrahepatic mait cells and histological features of nafld
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182470/
https://www.ncbi.nlm.nih.gov/pubmed/35683998
http://dx.doi.org/10.3390/nu14112198
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